Book contents
- Endophytes for a Growing World
- Endophytes for a Growing World
- Copyright page
- Contents
- Contributors
- Preface
- Part I Introduction
- Part II Role of Endophytes in Growth and Biotic and Abiotic Stress Resistance
- Part III Diversity and Community Ecology of Endophytes
- 8 Microbispora Dominate Diversity of Endophytic Actinobacteria from Australian Rice Plants
- 9 Isolation, Diversity and Potential Use of Endophytes in the Biomass and Bioenergy Crop Miscanthus
- 10 Life Within the Leaf: Ecology and Applications of Foliar Bacterial Endophytes
- 11 Meta-Omics Approach to Unravel the Endophytic Bacterial Communities of Brassica napus and Other Agronomically Important Crops in Response to Agricultural Practices
- 12 The Influence of Endophytes on Cork Oak Forests Under a Changing Climate
- Part IV Endophytes for Novel Biomolecules and In Vitro Methods
- Part V Application and Commercialisation of Endophytes in Crop Production
- Index
- References
9 - Isolation, Diversity and Potential Use of Endophytes in the Biomass and Bioenergy Crop Miscanthus
from Part III - Diversity and Community Ecology of Endophytes
Published online by Cambridge University Press: 01 April 2019
Book contents
- Endophytes for a Growing World
- Endophytes for a Growing World
- Copyright page
- Contents
- Contributors
- Preface
- Part I Introduction
- Part II Role of Endophytes in Growth and Biotic and Abiotic Stress Resistance
- Part III Diversity and Community Ecology of Endophytes
- 8 Microbispora Dominate Diversity of Endophytic Actinobacteria from Australian Rice Plants
- 9 Isolation, Diversity and Potential Use of Endophytes in the Biomass and Bioenergy Crop Miscanthus
- 10 Life Within the Leaf: Ecology and Applications of Foliar Bacterial Endophytes
- 11 Meta-Omics Approach to Unravel the Endophytic Bacterial Communities of Brassica napus and Other Agronomically Important Crops in Response to Agricultural Practices
- 12 The Influence of Endophytes on Cork Oak Forests Under a Changing Climate
- Part IV Endophytes for Novel Biomolecules and In Vitro Methods
- Part V Application and Commercialisation of Endophytes in Crop Production
- Index
- References
Summary
Endophytes have the potential to contribute to the sustainable production of bioenergy crops such as the perennial rhizomatous grass Miscanthus. They can improve plant growth on marginal land that is otherwise unsuitable for conventional agriculture and can also reduce the need for environmentally damaging chemical inputs including fertilisers and pesticides. This chapter outlines current knowledge of Miscanthus endophytes and presents new data on the diversity of root and shoot fungal endophytes isolated from three Miscanthus species (M. sacchariflorus, M. sinensis and M. ×giganteus). Malt extract, potato dextrose and Czapek Dox media were compared for isolation and growth of the endophytes. The endophytes were then identified using DNA barcoding with three DNA loci (nrITS, nrLSU and TEF). nrITS and nrLSU were found to be the most reliable and consistent barcoding regions. Internal transcribed spacer (ITS) had the highest discriminating potential and is thus recommended for single locus barcoding of endophytes in Miscanthus. Most new isolates were Ascomycota belonging to Pezizomycotina with representatives from Dothideomycetes, Eurotiomycetes and Sordariomycetes. One Basidiomycota species was recovered (a known soil yeast Rhodotorula). Comparisons between Miscanthus endophyte species composition and its better-known sister genus Saccharum (including sugarcane) are provided.
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- Information
- Endophytes for a Growing World , pp. 188 - 207Publisher: Cambridge University PressPrint publication year: 2019
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