Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Section one Overviews
- Section two Molecules for Chemical Genomics
- Section Three Basics of High-Throughput Screening
- Section Four Chemical Genomics Assays and Screens
- Chapter 12 Basics of HTS Assay Design and Optimization
- Chapter 13 Molecular Sensors for Transcriptional and Post-Transcriptional Assays
- Chapter 14 Time-Resolved Fluorescence Resonance Energy Transfer Technologies in HTS
- Chapter 15 Compound Profiling with High-Content Screening Methodology
- Chapter 16 Use of Transgenic Zebrafish in a Phenotypic Screen for Angiogenesis Inhibitors
- Chapter 17 Flow Cytometry Multiplexed Screening Methodologies
- Chapter 18 Label-Free Biosensor Technologies in Small Molecule Modulator Discovery
- Chapter 19 Basic Principles and Practices of Computer-Aided Drug Design
- Chapter 20 Computational Approach for Drug Target Identification
- Section five Chemical Genomics and Medicine
- Index
- References
Chapter 13 - Molecular Sensors for Transcriptional and Post-Transcriptional Assays
from Section Four - Chemical Genomics Assays and Screens
Published online by Cambridge University Press: 05 June 2012
- Frontmatter
- Contents
- Contributors
- Preface
- Section one Overviews
- Section two Molecules for Chemical Genomics
- Section Three Basics of High-Throughput Screening
- Section Four Chemical Genomics Assays and Screens
- Chapter 12 Basics of HTS Assay Design and Optimization
- Chapter 13 Molecular Sensors for Transcriptional and Post-Transcriptional Assays
- Chapter 14 Time-Resolved Fluorescence Resonance Energy Transfer Technologies in HTS
- Chapter 15 Compound Profiling with High-Content Screening Methodology
- Chapter 16 Use of Transgenic Zebrafish in a Phenotypic Screen for Angiogenesis Inhibitors
- Chapter 17 Flow Cytometry Multiplexed Screening Methodologies
- Chapter 18 Label-Free Biosensor Technologies in Small Molecule Modulator Discovery
- Chapter 19 Basic Principles and Practices of Computer-Aided Drug Design
- Chapter 20 Computational Approach for Drug Target Identification
- Section five Chemical Genomics and Medicine
- Index
- References
Summary
Cell-based assays allow researchers to probe the regulation of cell signaling, communication, and regulation within a system that is more physiologically relevant than assays that focus on isolated molecular components or cell extracts (biochemical assays). The application of cell-based assays in high-throughput screening (HTS) has increased in recent years. It is estimated that cell-based assays comprise more than half of HTS assays used in pharmaceutical industries [1].
Cell-based molecular sensor assays have been developed to measure either transcriptional or post-transcriptional events (Figure 13.1). A typical transcriptional reporter gene assay involves the placement of a cis-regulatory element in front of a gene that produces a measurable signal in response to modulation of transcriptional complexes that are regulated by ligands or complex networks. Assays favored in HTS are usually constructed to generate fluorescence or luminescence by expressing either an enzyme or a fluorescent protein reporter. Post-transcriptional assays have been developed by engineering the reporter to respond to events such as mRNA splicing, protein complex formation, translocation of a receptor between cellular compartments, or protein stability.
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- Information
- Chemical Genomics , pp. 173 - 197Publisher: Cambridge University PressPrint publication year: 2012