Book contents
- Frontmatter
- Contents
- Preface
- Foreword
- List of contributors
- 1 Coated vesicles: a morphologically distinct subclass of endocytic vesicles
- 2 Coated vesicles in different cell types: some functional implications
- 3 Coated vesicles: their occurrence in different plant cell types
- 4 Immunoglobulin transmission in mammalian young and the involvement of coated vesicles
- 5 Coated vesicles in neurons
- 6 Coated vesicles in the oocyte
- 7 Adsorptive and passive pinocytic uptake
- 8 Coated vesicles and receptor biology
- 9 Coated secretory vesicles
- 10 Dynamic aspects of coated vesicle function
- 11 Structural aspects of coated vesicles at the molecular level
- 12 Coated vesicles in medical science
- Appendix 1 Nomenclature
- Appendix 2 References added at proof
- Author index
- Subject index
- Plate section
11 - Structural aspects of coated vesicles at the molecular level
Published online by Cambridge University Press: 04 August 2010
- Frontmatter
- Contents
- Preface
- Foreword
- List of contributors
- 1 Coated vesicles: a morphologically distinct subclass of endocytic vesicles
- 2 Coated vesicles in different cell types: some functional implications
- 3 Coated vesicles: their occurrence in different plant cell types
- 4 Immunoglobulin transmission in mammalian young and the involvement of coated vesicles
- 5 Coated vesicles in neurons
- 6 Coated vesicles in the oocyte
- 7 Adsorptive and passive pinocytic uptake
- 8 Coated vesicles and receptor biology
- 9 Coated secretory vesicles
- 10 Dynamic aspects of coated vesicle function
- 11 Structural aspects of coated vesicles at the molecular level
- 12 Coated vesicles in medical science
- Appendix 1 Nomenclature
- Appendix 2 References added at proof
- Author index
- Subject index
- Plate section
Summary
The growth in our understanding in recent years of the class of pinocytic vesicles known as ‘coated vesicles’ has been substantial, as evidenced by the contributions of others to this volume. It is arguably in the sphere of biochemical and structural aspects of these vesicles, however, that this growth has been most rapid. It was only a few years ago, for example, that the cytoplasmic network on coated vesicles was thought to be merely protruding ‘bristles’ or ‘hairs’, or even that this structure observed in thin-sectioned material was an artifact of the tissue-processing procedure. Although some of the myths surrounding these ephemeral cytoplasmic organelles have been dispelled, there is still a long way to go before we can be fully confident of our interpretations of their structure and function.
Isolation of coated vesicles
Unlike the isolation of smooth pinosomes, the isolation of coated micropinocytic vesicles can be easily monitored by morphological methods, requiring only electron-microscopical observation for estimation of both purity and yield (Plate 1). In spite of this, it was not until 1969 that two groups of workers independently made attempts to isolate these subcellular organelles. The first group, that of Schjeide et al. (1969), isolated coated vesicles from oocytes by use of a simple pressing device, separating the vesicles from other components by density gradient centrifugation. The second group, that of Kanaseki & Kadota (1969), isolated vesicles from guinea pig brains.
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- Coated Vesicles , pp. 283 - 302Publisher: Cambridge University PressPrint publication year: 1980
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