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  • Cited by 6
Publisher:
Cambridge University Press
Online publication date:
June 2012
Print publication year:
2007
Online ISBN:
9780511807343

Book description

Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

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Contents

References
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Chapter 6
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Chapter 7
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Chapter 8
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Chapter 9
Bacteria
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Fungi
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Algae and plants
Bateman, J. M. & Purton, S. (2000). Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Molecular and General Genetics, 263, 404–410.
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Hellens, R. P., Edwards, E. A., Leyland, N. R., Bean, S. & Mullineaux, P. M. (2000). pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology, 42, 819–832.
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Wright, M., Dawson, J., Dunder, E., Suttie, J., Reed, J., Kramer, C., Chang, Y., Novitzky, R., Wang, H. & Artim-Moore, L. (2001). Efficient biolistic transformation of maize (Zea mays L.) and wheat (Triticum aestivum L.) using the phosphomannose isomerase gene, pmi, as the selectable marker. Plant Cell Reports, 20, 429–436.
Organelles
Bonnefoy, N. & Fox, T. D. (2001). Genetic transformation of Saccharomyces cerevisiae mitochondria. Methods in Cell Biology, 65, 381–396.
Lutz, K. A., Bosacchi, M. H. & Maliga, P. (2006). Plastid marker-gene excision by transiently expressed CRE recombinase. Plant Journal, 45, 447–456.
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Caenorhabditis elegans
Berezikov, E., Bargmann, C. I. & Plasterk, R. H. A. (2004). Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Research, 32, e40.
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Insects
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Rong, Y. S. & Golic, K. G. (2000). Gene targeting by homologous recombination in Drosophila. Science, 288, 2013–2018.
Schotta, G. & Reuter, G. (2000). Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system. Molecular and General Genetics, 262, 916–920.
Venken, K. J. T. & Bellen, H. J. (2005). Emerging technologies for gene manipulation in Drosophila melanogaster. Nature Reviews Genetics, 6, 167–178.
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Mammals
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Chapter 10
Uhlmann, E. J., Wong, M., Baldwin, R. L., Bajenaru, M. L., Onda, H., Kwiatkowski, D. J., Yamada, K. & Gutmann, D. H. (2002). Astrocyte-specific TSC1 conditional knockout mice exhibit abnormal neuronal organization and seizures. Annals of Neurology, 52, 285–296.

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