Countess Margery took as her second husband the Gentleman Pensioner Charles Tyrrell, sixth son of Sir Thomas Tyrrell of Heron, East Houndon, Essex, by Constance Blount, daughter of John Blount, Lord Mountjoy. (Charles's prior marriage to Agnes Chitwode alias Odell had been annulled by the Court of Delegates on 6 April 1560.) Although Margery and Charles are first identified as husband and wife in a will signed on 13 May 1566 by Charles's brother Richard of Assheton, Essex, as early as 11 October 1563 Margery, thanking Cecil for his ‘Gentylnes and fatherlye fryndshippe towerdes my Sunne’, refers to herself and ‘Mr Tyrell’ as ‘vs boothe’. The couple retained an interest in the Manor at Earls Colne, but lived principally in Kingston-upon-Thames, south-west of London. Charles's companions included ‘maister Iohn Seymore esquire one of the quenes Maiesties gentlemen pencioners and Mr Richarde Kelton gentleman … my freinde, maister Kelton of Colne’. An acquaintance was ‘Mr More clerke of our bande’ – another Gentleman Pensioner. Charles kept a ‘chamber at London’. Margery maintained friendships with the countesses of Warwick and Worcester, as with Charles's three sisters and one of their husbands: ‘sister Churche’; ‘brother and sister Garnisshe’; and ‘sister Felton’. One of Margery's gentlewomen-servants was a ‘Mrs Gardener’.

As for Oxford, on 9 January 1564 William Cecil wrote to the Countess of Rutland:

Hitcham (Berkshire), where Oxford was recuperating from an illness, lies about twenty-five miles west of London, between Slough and Maidenhead. Oxford and Rutland would keep close company over the next three years.

In August the Queen visited Cambridge, arriving on Saturday the 5th and leaving on Thursday the 10th. St John's College accommodated Cecil, Oxford, and Rutland – for this was Cecil's alma mater.