Contents

pp v-vi

Contributors

pp vii-x

Foreword by Russell Higuchi

pp xi-xii

Preface

pp xiii-xviii

I - BASIC TECHNOLOGIES

pp 1-2

1 - Real-time polymerase chain reaction

pp 3-11

2 - Thermostable enzymes used in polymerase chain reaction

pp 12-18

3 - Inventing molecular beacons

pp 19-47

4 - Rapid polymerase chain reaction and melting analysis

pp 48-69

5 - Polymerase chain reaction and fluorescence chemistries: deoxyribonucleic acid incarnate

pp 70-79

6 - Analysis of microribonucleic acid expression by quantitative real-time polymerase chain reaction

pp 80-87

7 - Miniaturized polymerase chain reaction for quantitative clinical diagnostics

pp 88-109

8 - The road from qualitative to quantitative assay: What is next?

pp 110-128

9 - Taking control of the polymerase chain reaction

pp 129-152

II - APPLICATIONS

pp 153-154

10 - Polymerase chain reaction–based methods for the detection of solid tumor cancer cells for clinical diagnostic and prognostic assays

pp 155-172

11 - Polymerase chain reaction and infectious diseases

pp 173-188

12 - Polymerase chain reaction and respiratory viruses

pp 189-211

13 - Polymerase chain reaction and severe acute respiratory syndrome

pp 212-228

14 - The MMR vaccine, measles virus, and autism – A cautionary tale

pp 229-242

15 - Noninvasive prenatal diagnosis using cell-free fetal nucleic acids in maternal plasma

pp 243-253

16 - Polymerase chain reaction–based analyses of nucleic acids from archival material

pp 254-261

17 - Microarrays and quantitative real-time reverse transcriptase–polymerase chain reaction

pp 262-275

18 - Polymerase chain reaction in the detection of genetic variation

pp 276-283

19 - Polymerase chain reaction: A blessing and a curse for ancient deoxyribonucleic acid research

pp 284-300

Index

pp 301-307

Plate section