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Abnormalities in extracellular glycine and glutamate levels in the striatum of sandy mice

Published online by Cambridge University Press:  26 February 2013

Yuji Kitaichi ,
Ryota Hashimoto ,
Takeshi Inoue ,
Tomohiro Abekawa ,
Aya Kakuta ,
Satoko Hattori  and
Tsukasa Koyama
Yuji Kitaichi
Affiliation:
Department of Psychiatry, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan
Ryota Hashimoto*
Affiliation:
Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Osaka, Japan Department of Psychiatry, Osaka University Graduate School of Medicine, Osaka, Japan Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan
Takeshi Inoue
Affiliation:
Department of Psychiatry, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan
Tomohiro Abekawa
Affiliation:
Department of Psychiatry, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan
Aya Kakuta
Affiliation:
Department of Psychiatry, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan
Satoko Hattori
Affiliation:
Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan
Tsukasa Koyama
Affiliation:
Department of Psychiatry, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo, Japan
*
Dr. Ryota Hashimoto, Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, D3, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan. Tel: +81-6-6879-3074; Fax: +81-6-6879-3074; E-mail: hashimor@psy.med.osaka-u.ac.jp
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Abstract

Objective

Glycine regulates glutamatergic neurotransmission, and several papers have reported the relationship between glycine and schizophrenia. The dysbindin-1 (DTNBP1: dystrobrevin-binding protein 1) gene is related to glutamatergic neurotransmission and has been found to be a strong candidate gene for schizophrenia. In this study, we clarified the relationship between dysbindin, glutamate, and glycine with in vivo microdialysis methods.

Methods

We measured extracellular glycine and glutamate levels in the striatum of sandy (sdy) mice using in vivo microdialysis methods. Sdy mice express no dysbindin protein owing to a deletion in the dysbindin-1 gene. In addition, we measured changes in those amino acids after methamphetamine (METH) administration.

Results

The basal levels of extracellular glycine and glutamate in the striatum of sdy mice were elevated. These extracellular glutamate levels decreased gradually after METH administration and were not subsequently different from those of wild-type mice.

Conclusions

These results suggest that dysbindin might modulate glycine and glutamate release in vivo.

Keywords

dysbindinglutamateglycinemethamphetaminemicrodialysis
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 25 , Issue 4 , August 2013 , pp. 215 - 220
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

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