Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T04:59:57.802Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA fragment mass distributions following molecular recombination

Published online by Cambridge University Press:  01 July 2016

Thomas A. Darden  and
Michael A. Resnick
Thomas A. Darden*
Affiliation:
National Institute of Environmental Health Sciences
Michael A. Resnick*
Affiliation:
National Institute of Environmental Health Sciences
*
∗Postal address: Biometry and Risk Assessment Program, NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, USA.
∗∗Postal address: Toxicology Research and Testing Program, NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, USA.
Get access Rights & Permissions [Opens in a new window]

Abstract

A mathematical model for the process of recombinational repair of DNA damage is presented. Based on the model, a method is proposed for analyzing fragment mass distributions from experiments designed to detect recombinational repair in cells. The procedures developed can be used to analyze experiments involving sucrose-gradient measurements of mass distributions. The model also provides a framework for discussion of various molecular models of this repair process.

Keywords

DNA DAMAGE
Type
Research Article
Information
Advances in Applied Probability , Volume 17 , Issue 3 , September 1985 , pp. 496 - 513
Copyright
Copyright © Applied Probability Trust 1985 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bithell, J. F. (1969) A stochastic model for the breaking of molecular segments. J. Appl. Prob. 6, 59–73.Google Scholar
Bithell, J. F. and Wahrendorf, J. (1982) Estimation of the true length of broken molecules. Biometrics 38, 201–213.Google Scholar
Burgi, E. and Hershey, A. D. (1963) Sedimentation rate as a measure of molecular weight of DNA. Biophys. J. 3, 309–321.Google Scholar
Crothers, D. M., Spatz, H. and Elson, E. (1969) Computation of molecular weight averages for DNA molecules containing both preformed and randomly induced single strand breaks. Biopolymers 7, 215–221.Google Scholar
Ehmann, U. K. and Lett, J. T. (1973) Review and evaluation of molecular weight calculations from the sedimentation profiles of irradiated DNA. Radiation Res. 54, 152–162.CrossRefGoogle ScholarPubMed
Ganesan, A. (1974) Persistence of pyrimidine dimers during post-replication repair in ultraviolet light irradiated E. coli K-12. J. Mol. Biol. 87, 103–119.Google Scholar
Karlin, S. (1966) A First Course in Stochastic Processes. Academic Press, New York.Google Scholar
Litwin, S. (1969) The distribution of radioactive recovery in randomly cut and sedimented DNA. J. Appl. Prob. 6, 275–284.CrossRefGoogle Scholar
Litwin, S., Shahn, E. and Kozinski, A. (1969) Interpretation of sucrose gradient sedimentations pattern of deoxyribonucleic acid fragments resulting from random breaks. J. Virology 4, 24–30.Google Scholar
Meselson, M. (1972) Formation of hybrid DNA by rotary diffusion during genetic recombination. J. Mol. Biol. 71, 795–798.Google Scholar
Paterson, M. D. (1978) The use of purified lesion recognizing enzymes to monitor DNA repair in vivo. Advances in Radiation Biology 7. Academic Press, New York.Google Scholar
Resnick, M. A. (1979) The induction of molecular and genetic recombination in eukaryotes. Advances in Radiation Biology 8. Academic Press, New York.Google Scholar
Resnick, M. A., Stasiewicz, S. and Game, J. C. (1983) Meiotic DNA metabolism in normal and excision deficient yeast following UV exposure. Genetics 104, 583–601.CrossRefGoogle ScholarPubMed
Resnick, M. A. and Darden, T. (1983) The relationship between DNA damage and recombination: A mathematic analysis. In preparation.Google Scholar
Zimm, B. H. (1974) Anomalies in sedimentation IV. Decrease in sedimentation coefficient of chains at high fields. Biophys. Chem. 1, 279–291.Google Scholar