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Calibration and Correction Methods for Quantitative Proton-Induced X-Ray Emission Analysis of Autopsy Tissues

Published online by Cambridge University Press:  06 March 2019

K. K. Nielson ,
M. W. Hill  and
N. F. Mangelson
K. K. Nielson*
Affiliation:
Chemistry and Physics Departments and Center for Thermochemical Studies Brigham Young University, Provo, Utah 84602
M. W. Hill
Affiliation:
Chemistry and Physics Departments and Center for Thermochemical Studies Brigham Young University, Provo, Utah 84602
N. F. Mangelson
Affiliation:
Chemistry and Physics Departments and Center for Thermochemical Studies Brigham Young University, Provo, Utah 84602
*
†Present address: Battelle-Northwest, Richland, Washington 99352.
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Abstract

Methods have been developed for autopsy tissue analysis using a proton-induced X-ray emission (PIXE) system optimized for thin sample analysis. The system uses 2 MeV protons, thus limiting sample thickness to several milligrams per square centimeter. Calibration was accomplished with standard solutions spotted onto Nuclepore filters, which were subsequently irradiated in a uniform proton flux. X-ray yields measured with a Si (Li) detector were corrected for proton energy loss in the filter matrix as well as X-ray attenuation. Corrections for proton energy loss were determined from empirical parameters relating proton energy to X-ray cross sections. Typical filter thickness and penetration of the sample solution into the filter matrix were measured allowing calculation of proton energy attenuation and X-ray absorption corrections. The method was used in routine analyses for sixteen elements in seven types of human tissue. Accuracy was evaluated with standard reference materials and atomic absorption analyses.

Type
X-Ray Spectrometry in Biomedical Applications
Information
Advances in X-Ray Analysis , Volume 19: Twenty-Fourth Annual Conference on Applications of X-ray Analysis, August 6-8, 1975 , 1975 , pp. 511 - 520
Copyright
Copyright © International Centre for Diffraction Data 1975

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References

1. Walter, R. L., Willis, E. D., Gutknecht, W. F. and Joyce, J. M., “Analysis of Biological, Clinical and Environmental Samples Using Proton-Induced X-Ray Emission,” Anal. chem., 46, 843–855 (1974).Google Scholar
2. Kaufmann, H. C. and. Akselsson, R., “Non-Linear Least Squares Analysis of Proton-Induced X-Ray Emission Data,” Advances in X-Ray Analysis 18, 353–361 (1975).Google Scholar
3. Johansson, T. B., VanGrieken, R. E., Nelson, J. W. and Winchester, J. W., “Elemental Trace Analysis of Small Samples by Proton Induced X-Ray Emission,” Anal. Chem., 47, 855–860 (1975).Google Scholar
4. Johansson, T. B., Private Communication, 1973.Google Scholar
5. Mangelson, N. F., Hill, M. W., Nielson, K. K., Eatough, D. J., Christensen, J. J. and lzatt, R. M., “Proton Induced X-Ray Emission Analysis of Pima Indian Autopsy Tissues,” Brigham Young University, Provo, UT., 1975, (to be published.).Google Scholar
6. Bearse, R. C., Close, D. A., Malanify, J. J. and Umbarger, C. J., “Production of Kα and Lα X-Rays by Protons of 1.0-3.7 MeV,” Phys. Rev. A 7, 1269-1272 (1973).Google Scholar
7. Akselsson, R. and Johansson, T. B., “X-Ray Production by 1.5 - 11 MeV Protons,” Z. Physik, 266, 245–255(1974).Google Scholar
8. Nielson, K. K., “Development of Proton-Indueed X-Ray Emission Tech¬niques with Application to Multielement Analyses of Human Autopsy Tissues and Obsidian Artifacts,” Ph.P. Dissertation, Dept. of Chem., Brigham Young University, Provo, UT ﹛1975).Google Scholar
9. Williamson, C. F., Boujot, J. P. and Picard, J., “Tables of Range and Stopping Power of Chemical Elements for Charged Particles of Energy 0.05 to 500 MeV,” Commissariat a l'Energie Atomique-France, Report CEA-R 3042 (1966).Google Scholar
10. Connelly, A. L. and Black, W. W., “Automatic Location and Area Determination of Photopeaks,” Nucl. Inst. Meth. 82, l4l–l48 (1970).Google Scholar
11. Harrison, J. F. and Eldred, E. A., “Automatic Data Acquisition and Reduction for Elemental Analysis of Aerosol Samples,” Advances in X-Ray Anlaysis 17, 560–570 (1973).Google Scholar