Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-19T09:27:11.981Z Has data issue: false hasContentIssue false
  • English
  • Français

Retention of copper in the liver of sheep genetically selected for high and low concentrations of copper in plasma

Published online by Cambridge University Press:  02 September 2010

J. A. Woolliams ,
G. Wiener ,
Carol Woolliams  and
N. F. Suttle
J. A. Woolliams
Affiliation:
AFRC Animal Breeding Research Organisation, West Mains Road, Edinburgh EH9 3JQ
G. Wiener
Affiliation:
AFRC Animal Breeding Research Organisation, West Mains Road, Edinburgh EH9 3JQ
Carol Woolliams
Affiliation:
AFRC Animal Breeding Research Organisation, West Mains Road, Edinburgh EH9 3JQ
N. F. Suttle
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH
Get access

Abstract

The extent to which genetic selection to change the concentration of copper (Cu) in plasma also affected the retention of Cu in the liver was assessed in three experiments. In each, the concentration of Cu was measured in samples of liver (taken by biopsy) and of plasma from female sheep of lines selected from a Scottish Blackface × Welsh Mountain population (which had previously been interbred for several generations) for high and low plasma Cu levels (high line and low line) and from purebred Scottish Blackface and Welsh Mountain females.

The concentration of Cu in the liver and plasma was found to be consistently greater in high-line and Welsh Mountain than in low-line and Scottish Blackface females (P < 0·01) in the first experiment in which grazing animals were sampled at both 6 and 10 months of age and in the second, where sampling' was at 6 months only. Mean Cu concentrations in the liver ranged from 13 to 115 mg/kg dry matter (DM) and the correlations with those in plasma varied from 0·28 to 0·53 (all P < 0·05) and was highest when the concentration of Cu in the liver was lowest. The correlation between the concentrations of Cu in liver at different ages was 0·57 (P < 0·05).

In the third experiment, 10-month-old high- and low-line females were given complete diets containing either 5, 10, 22 or 28 mg Cu per kg DM for 12 weeks. Liver samples were obtained at the beginning and end of this period and blood samples were taken every 3 weeks. The concentration of Cu in the liver was greater in high- than in low-line sheep on all diets and increased linearly with the Cu concentration of the diet but at a greater rate in the high line. At the end, the high line had significantly greater concentrations of Cu in plasma on the 5 and 10 mg Cu per kg DM diets.

The experiments showed that high-line females retained more Cu in the liver than low-line females. It is most likely that this was caused by a positive genetic correlation between concentration of Cu in plasma and the efficiency of absorption of Cu.

Type
Research Article
Information
Animal Production , Volume 41 , Issue 2 , October 1985 , pp. 219 - 226
Copyright
Copyright © British Society of Animal Science 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Grace, N. D. and Suttle, N. F. 1979. Some effects of sulphur intake on molybdenum metabolism in sheep. Br. J. Nutr. 41: 125–136.CrossRefGoogle ScholarPubMed
Hammond, J. 1932. Growth and the Development of Mutton Qualities in the Sheep, pp. 112–138. 2nd ed. Oliver and Boyd, Edinburgh.Google Scholar
Ministry of Agriculture, Fisheries and Food. 1982. Report by ADAS/ARCICSG Working Party on Copper in Ruminant Animal Nutrition. Ministry of Agriculture, Fisheries and Food, London.Google Scholar
Suttle, N. F. 1974. A technique for measuring the biological availability of copper to sheep using hypocupraemic ewes. Br. J. Nutr. 32: 395–405.CrossRefGoogle ScholarPubMed
Suttle, N. F., Jones, D. G., Woolliams, J. A., Woolliams, C. and Wiener, G. 1984. Growth responses to copper and selenium in lambs of different breeds on improved hill pastures. Proc. Nutr. Soc. 43: 103A.Google Scholar
Tallis, G. M. 1961. The moment generating function of the truncated multi-normal distribution. Jl R. statist. Soc. 23B: 223–229.Google Scholar
Thompson, R. H. and Blanchflower, W. J. 1971. Wet-ashing apparatus to prepare biological materials for atomic absorption spectrophotometry. Lab. Pract. 20: 859–861.Google ScholarPubMed
Wallace, L. R. 1948. The growth of lambs before and after birth in relation to the level of nutrition. Part II. J. agric. Sci., Camb. 38: 243–302.CrossRefGoogle Scholar
Wiener, G. and Field, A. C. 1969. Copper concentrations in the liver and blood of sheep of different breeds in relation to swayback history. J. comp. Path. 79: 7–14.CrossRefGoogle ScholarPubMed
Wiener, G., Herbert, J. G. and Field, A. C. 1976. Variation in liver and plasma copper concentrations of sheep in relation to breed and haemoglobin type. J. comp. Path. 86: 101–109.CrossRefGoogle ScholarPubMed
Wiener, G., Suttle, N. F., Field, A. C., Herbert, J. G. and Woolliams, J. A. 1978. Breed differences in copper metabolism in sheep. J. agric. Sci., Camb. 91: 433–441.CrossRefGoogle Scholar
Wiener, G., Woolliams, J. A., Woolliams, Carol and Field, A. C. 1985. Genetic selection to produce lines of sheep true correlated differing in plasma copper concentrations. Anim. Prod. 40: 465–473.Google Scholar
Woolliams, J. A., Suttle, N. F., Wiener, G., Field, A. C. and WOOLLIAMS, CAROL. 1982. The effect of breed of sire on the accumulation of copper in lambs, with particular reference to copper toxicity. Anim. Prod. 35: 299–307.Google Scholar
Woolliams, J. A., Suttle, N. F., Wiener, G., Field, A. C. and Woolliams, Carol. 1983. The long-term accumulation and depletion of copper in the liver of different breeds of sheep fed diets of differing copper content. J. agric. Sci., Camb. 100: 441–449.CrossRefGoogle Scholar
Woolliams, J. A., Woolliams, Carol, Wiener, G., Jones, D. G. and Suttle, N. F. 1984. An association between lamb mortality and copper status in different breedsof sheep. Proc. Nutr. Soc. 43:102A.Google Scholar