Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-24T03:46:16.308Z Has data issue: false hasContentIssue false
  • English
  • Français

Black soldier fly larva fat inclusion in finisher broiler chicken diet as an alternative fat source

Published online by Cambridge University Press:  18 January 2018

A. Schiavone ,
S. Dabbou ,
M. De Marco ,
M. Cullere ,
I. Biasato ,
E. Biasibetti ,
M. T. Capucchio ,
S. Bergagna ,
D. Dezzutto  and
M. Meneguz
...Show all authors
A. Schiavone
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy Institute of Science of Food Production, National Research Council, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
S. Dabbou
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
M. De Marco
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
M. Cullere
Affiliation:
Department of Animal Medicine, Production and Health, University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, Padova, Italy
I. Biasato
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
E. Biasibetti
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
M. T. Capucchio
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
S. Bergagna
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and Valle d’Aosta, Via Bologna 148, 10154 Turin, Italy
D. Dezzutto
Affiliation:
Veterinary Medical Research Institute for Piemonte, Liguria and Valle d’Aosta, Via Bologna 148, 10154 Turin, Italy
M. Meneguz
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
F. Gai
Affiliation:
Institute of Science of Food Production, National Research Council, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
A. Dalle Zotte*
Affiliation:
Department of Animal Medicine, Production and Health, University of Padova, Agripolis, Viale dell’Università 16, 35020 Legnaro, Padova, Italy
L. Gasco
Affiliation:
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy Institute of Science of Food Production, National Research Council, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
*
†E-mail: antonella.dallezotte@unipd.it
Get access

Abstract

The objective of the present study was to evaluate the effects of partial or total replacement of finisher diet soybean oil with black soldier fly (Hermethia illucens L.; HI) larva fat on the growth performance, carcass traits, blood parameters, intestinal morphology and histological features of broiler chickens. At 21 days of age, a total of 120 male broiler chickens (Ross 308) were randomly allocated to three experimental groups (five replicates and eight birds/pen). To a basal control diet (C; 68.7 g/kg as fed of soybean oil), either 50% or 100% of the soybean oil was replaced with HI larva fat (HI50 and HI100 group, respectively). Growth performance was evaluated throughout the trial. At day 48, 15 birds (three birds/pen) per group were slaughtered at a commercial abattoir. Carcass yield and proportions of carcass elements were recorded. Blood samples were taken from each slaughtered chicken for haematochemical index determination. Morphometric analyses were performed on the duodenum, jejunum and ileum. Samples of liver, spleen, thymus, bursa of fabricius, kidney and heart were submitted to histological investigations. Growth performance, carcass traits, haematochemical parameters and gut morphometric indexes were not influenced by the dietary inclusion of HI larva fat. Histopathological alterations developed in the spleen, thymus, bursa of fabricius and liver and were identified in all of the experimental groups, but HI larva fat inclusion did not significantly affect (P>0.05) the severity of the histopathological findings. The present study suggests that 50% or 100% replacement of soybean oil with HI larva fat in broiler chickens diets has no adverse effects on growth performance or blood parameters and had no beneficial effect on gut health.

Keywords

Hermetia illucensbroiler chickensdietary fat sourcegrowth performancegut morphometry
Type
Research Article
Information
animal , Volume 12 , Issue 10 , October 2018 , pp. 2032 - 2039
Copyright
© The Animal Consortium 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Association of Official Analytical Chemists 2005. Official methods of analysis, 18th edition. AOAC, Washington, DC, USA.Google Scholar
Aviagen 2014. Ross 708 broiler. Broiler performance objectives. Retrieved on 10 May 2017 from http://en.avi-agen.com/ross-708/.Google Scholar
Barroso, FG, de Haro, C, Sánchez-Muros, MJ, Venegas, E, Martínez-Sánchez, A and Pérez-Bañón, C 2014. The potential of various insect species for use as food for fish. Aquaculture 422, 193–201.Google Scholar
Belforti, M, Gai, F, Lussiana, C, Renna, M, Marfatto, V, Rotolo, L, De Marco, M, Dabbou, S, Schiavone, A, Zoccarato, I and Gasco, L 2015. Tenebrio molitor meal in rainbow trout (Oncorhynchus mykiss) diets: effects on animal performance, nutrient digestibility and chemical composition of fillets. Italian Journal of Animal Science 14, 670–676.Google Scholar
Biasato, I, De Marco, M, Rotolo, L, Renna, M, Dabbou, S, Capucchio, MT, Biasibetti, E, Tarantola, M, Costa, P, Gai, F, Pozzo, L, Dezzutto, D, Bergagna, S, Gasco, L and Schiavone, A 2016. Effects of dietary Tenebrio molitor meal inclusion in free-range chickens. Journal of Animal Physiology and Animal Nutrition 100, 1104–1112.Google Scholar
Cullere, M, Tasoniero, G, Giaccone, V, Miotti-Scapin, R, Claeys, E, De Smet, S and Dalle Zotte, A 2016. Black soldier fly as dietary protein source for broiler quails: apparent digestibility, excreata microbial load, feed choice, performance, carcass and meat traits. Animal 10, 1923–1930.Google Scholar
Cunnick, JE, Kojic, LD and Hughes, RA 1994. Stress induced changes in immune function are associated with increased production of an interleukin-1-like factor in young domestic fowl. Brain, Behavior and Immunity 8, 123–136.Google Scholar
De Marco, M, Martinez, S, Tarantola, M, Bergagna, S, Mellia, E, Gennero, MS and Schiavone, A 2013. Effect of genotype and transport on tonic immobility and heterophil/lymphocyte ratio in two local Italian breeds and Isa Brown hens kept under free-range conditions. Italian Journal of Animal Science 12, 481–485.Google Scholar
de Verdal, H, Mignon-Grasteau, S, Jeulin, C, Le Bihan-Duval, E, Leconte, M, Mallet, S, Martin, C and Narcy, A 2010. Digestive tract measurements and histological adaptation in broiler lines divergently selected for digestive efficiency. Poultry Science 89, 1955–1961.Google Scholar
Fascina, VB, Carrijo, AS, Souza, KMR, Garcia, AML, Kiefer, C and Sartori, JR 2009. Soybean oil and beef tallow in starter broiler diets. Brazilian Journal of Poultry Science 11, 249–256.Google Scholar
Gasco, L, Henry, M, Piccolo, G, Marono, S, Gai, F, Renna, M, Lussiana, C, Antonopoulou, F, Mola, P and Chatzifotis, S 2016. Tenebrio molitor meal in diets for European sea bass (Dicentrarchus labrax, L.) juveniles: growth performance, whole body composition and in vivo apparent digestibility. Animal Feed Science and Technology 220, 34–45.Google Scholar
Heckert, RA, Estevez, I, Russek-Cohen, E and Pettit-Riley, R 2002. Effects of density and perch availability on the immune status of broilers. Poultry Science 81, 451–457.Google Scholar
Hyder, MA, Hasan, M and Mohieldein, AH 2013. Comparative levels of ALT, AST, ALP and GGT in liver associated diseases. European Journal of Experimental Biology 3, 280–284.Google Scholar
Kappally, S, Shirwaikar, A and Shirwaikar, A 2015. Coconut oil: a review of potential applications. Hygeia Journal of Drugs and Medicine 7, 34–41.Google Scholar
Laudadio, V, Passantino, L, Perillo, A, Lopresti, G, Passantino, A, Khan, RU and Tufarelli, V 2012. Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poultry Science 91, 265–270.Google Scholar
Leiber, F, Gelencsér, T, Stamer, A, Amsler, Z, Wohlfahrt, J, Früh, B and Maurer, V 2017. Insect and legume-based protein sources to replace soybean cake in an organic broiler diet: Effects on growth performance and physical meat quality. Renewable Agriculture and Food Systems 32, 21–27.Google Scholar
Li, L, Ji, H, Zhang, B, Tian, J, Zhou, J and Yu, H 2016. Influence of black soldier fly (Hermetia illucens) larvae oil on growth performance, body composition, tissue fatty acid composition and lipid deposition in juvenile Jian carp (Cyprinus carpio var. Jian). Aquaculture 465, 43–52.Google Scholar
Mattioli, S, Dal Bosco, A, Szendrő, ZS, Cullere, M, Gerencsér, ZS, Matics, ZS, Castellini, C and Dalle Zotte, A 2016. The effect of dietary Digestarom® herbal supplementation on rabbit meat fatty acid profile, lipid oxidation and antioxidant content. Meat Science 121, 238–242.Google Scholar
Ozdogan, M, Topal, E, Paksuz, EP and Kirkan, S 2014. Effect of different levels of crude glycerol on the morphology and some pathogenic bacteria of the small intestine in male broilers. Animal 8, 36–42.Google Scholar
Pozzo, L, Salamano, G, Mellia, E, Gennero, MS, Doglione, L, Cavallarin, L, Tarantola, M, Forneris, G and Schiavone, A 2013. Feeding a diet contaminated with ochratoxin A for broiler chickens at the maximum level recommended by the EU for poultry feeds (0.1 mg/kg). 1. Effects on growth and slaughter performance, haematological and serum traits. Journal of Animal Physiology and Animal Nutrition 97, 13–22.Google Scholar
Qaisrani, SN, Moquet, PC, van Krimpen, MM, Kwakkel, RP, Verstegen, MW and Hendriks, WH 2014. Protein source and dietary structure influence growth performance, gut morphology, and hind gut fermentation characteristics in broilers. Poultry Science 93, 3053–3064.Google Scholar
Ramos-Elorduy, J 1997. Insects: a sustainable source of food? Ecology of Food and Nutrition 36, 247–276.Google Scholar
Renna, M, Schiavone, A, Gai, F, Dabbou, S, Lussiana, C, Malfatto, V, Prearo, M, Capucchio, MT, Biasato, I, Biasibetti, E, De Marco, M, Brugiapaglia, A, Zoccarato, I and Gasco, L 2017. Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets. Journal of Animal Science and Biotechnology 8, 57.Google Scholar
Rumpold, BA, Klocke, M and Schlüter, O 2016. Insect biodiversity: underutilized bioresource for sustainable applications in life sciences. Regional Environmental Change 17, 1445-1454.Google Scholar
Salamano, G, Mellia, E, Tarantola, M, Gennero, MS, Doglione, L and Schiavone, A 2010. Acute phase proteins and heterophil:lymphocyte ratio in laying hens in different housing systems. Veterinary Record 167, 749–751.Google Scholar
Schiavone, A, Chiarini, R, Marzoni, M, Castillo, A, Tassone, S and Romboli, I 2007. Breast meat traits of Muscovy ducks fed on a microalga (Crypthecodinium cohnii) meal supplemented diet. British Poultry Science 48, 573–579.Google Scholar
Schiavone, A, Cullere, M, De Marco, M, Meneguz, M, Biasato, I, Bergagna, S, Dezzutto, D, Gai, F, Dabbou, S, Gasco, L and Dalle Zotte, A 2017a. Partial or total replacement of soybean oil by black soldier larvae (Hermetia illucens L.) fat in broiler diets: effect on growth performances, feed-choice, blood traits, carcass characteristics and meat quality. Italian Journal of Animal Science 16, 93–100.Google Scholar
Schiavone, A, De Marco, M, Martínez, S, Dabbou, S, Renna, M, Madrid, J, Hernandez, F, Rotolo, L, Costa, P, Gai, F and Gasco, L 2017b. Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens: apparent nutrient digestibility, apparent metabolizable energy and apparent ileal amino acid digestibility. Journal of Animal Science and Biotechnology 8, 51.Google Scholar
Surendra, KC, Olivier, R, Tomberlin, JK, Jha, R and Khanal, SK 2016. Bioconversion of organic wastes into biodiesel and animal feed via insect farming. Renewable Energy 98, 197-202.Google Scholar
Tran, G, Heuzé, V and Makkar, HPS 2015. Insects in fish diets. Animal Frontiers 5, 37–44.Google Scholar
Ushakova, NA, Brodskii, ES, Kovalenko, AA, Bastrakov, AI, Kozlova, AA and Pavlov, ADS 2016. Characteristics of lipid fractions of larvae of the black soldier fly Hermetia illucens . Doklady Biochemistry and Biophysics 468, 209–212.Google Scholar
Veldkamp, T and Bosch, G 2015. Insects: a protein-rich feed ingredient in pig and poultry diets. Animal Frontiers 5, 45–50.Google Scholar
Wang, J, Wang, X, Li, J, Chen, Y, Yang, W and Zhang, L 2015. Effects of dietary coconut oil as a medium-chain fatty acid source on performance, carcass composition and serum lipids in male broilers. Asian-Australasian Journal of Animal Sciences 28, 223–230.Google Scholar
Wang, JX and Peng, KM 2008. Developmental morphology of the small intestine of African ostrich chicks. Poultry Science 87, 2629–2635.Google Scholar
Zeitz, J, Fennhoff, OJ, Kluge, H, Stangl, GI and Eder, K 2015. Effects of dietary fats rich in lauric and myristic acid on performance, intestinal morphology, gut microbes, and meat quality in broilers. Poultry Science 94, 2404–2413.Google Scholar