Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-19T07:23:56.395Z Has data issue: false hasContentIssue false

Cloning, molecular characterization, and spatial and developmental expression analysis of GPR41 and GPR43 genes in New Zealand rabbits

Published online by Cambridge University Press:  28 February 2017

C. Y. Fu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
L. Liu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
Q. Gao
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
X. Y. Sui
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
F. C. Li*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong 271018, China
*
E-mail: chlf@sdau.edu.cn
Get access

Abstract

Short-chain fatty acids (SCFAs) play a regulatory role in various physiological processes in mammals and act as endogenous ligands for the G protein-coupled receptors (GPR) 41 and 43. The role of GPR41 and GPR43 in mediating SCFA signaling in the rabbit remains unclear. The present study was to investigate the sequence of the GPR41 and GPR43 messenger RNA (mRNA) and their expression pattern in different tissues and developmental stages in New Zealand rabbit. Comparison of genomic sequences in GenBank using the Basic Local Alignment Search Tool program suggested that the New Zealand rabbit GPR41 mRNA has high similarities with the human (84%), bovine (84%) and Capra hircus (84%) genes. Similarly, GPR43 mRNA has high similarity with the rat (84%) and mouse (84%) genes. Real-time PCR results indicated that GPR41 and GPR43 mRNA were expressed throughout rabbit’s whole development and were expressed in several tissues. G protein-coupled receptor 41 and GPR43 mRNA were most highly expressed in pancreas (P<0.05) and s.c. adipose tissue (P<0.05), respectively. The expression levels of GPR41 mRNA was down-regulated in duodenum, cecum (P<0.05) and pancreas and up-regulated in jejunum, ileum, adipose tissue and spleen during growth. G protein-coupled receptor 43GPR43 mRNA was highly expressed in the duodenum, jejunum, ileum, colon, cecum and lung at 15th day (P<0.05), whereas the expression levels in the pancreas and spleen increased later after birth, with the highest expression at 60th day (P<0.05).

Type
Research Article
Copyright
© The Animal Consortium 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Co-first authors.

References

Al-Lahham, SH, Peppelenbosch, MP, Roelofsen, H, Vonk, RJ and Venema, K 2010. Biological effects of propionic acid in humans; metabolism, potential applications and underlying mechanisms. Biochimica et Biophysica Acta – Molecular and Cell Biology of Lipids 1801, 11751183.CrossRefGoogle ScholarPubMed
Brown, AJ, Goldsworthy, SM, Barnes, AA, Eilert, MM, Tcheang, L, Daniels, D, Muir, AI, Wigglesworth, MJ, Kinghorn, I, Fraser, NJ, Pike, NB, Strum, JC, Steplewski, KM, Murdock, PR, Holder, JC, Marshall, FH, Szekeres, PG, Wilson, S, Ignar, DM, Foord, SM, Wise, A and Dowell, SJ 2003. The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids. The Journal of Biological Chemistry 278, 1131211319.Google Scholar
Bourdu, S, Dapoigny, M, Chapuy, E, Artigue, F, Vasson, MP, Eechelotte, P, Bommelaer, G, Eschalier, A and Ardid, D 2005. Rectal instillation of butyrate provides a novel clinically relevant model of noninflam-matory colonic hypersensitivity in rats. Gastroenterology 128, 19962008.Google Scholar
Covington, DK, Briscoe, CA, Brown, AJ and Jayawickreme, CK 2006. The G-protein-coupled receptor 40 family (GPR40-GPR43) and its role in nutrient sensing. Biochemical Society Transactions 34, 770773.CrossRefGoogle ScholarPubMed
de Blas, JC and Mateos, GG 1998. Feed formulation. In The nutrition of rabbit (ed. C de Blas and J Wiseman), pp. 241–253. CABI Publishing, New York, NY, USA.Google Scholar
Dorota, M, Bożena, K, Milan, M, Ewa, P, Wojciech, Z and Józef, N 2015. In vitro study and comparison of caecal methanogenesis and fermentation pattern in the brown hare (Lepus europaeus) and domestic rabbit (Oryctolagus cuniculus). PLoS One 10, e0117117.Google Scholar
Freeman, TC, Bentsen, BS, Thwaites, DT and Simmons, NL 1995. H+/di-tripeptide trans-porter (PepT1) expression in the rabbit intestine. Pflugers Archiv: European Journal of Physiology 430, 394400.Google Scholar
Ge, H, Li, X, Weiszmann, J, Wang, P, Baribault, H, Chen, JL and Li, Y 2008. Activation of G protein-coupled receptor 43 in adipocytes leads to inhibition of lipolysis and suppression of plasma free fatty acids. Endocrinology 149, 45194526.Google Scholar
Guilloteau, P, Martin, L, Eeckhaut, V and Ducatelle, R 2010. From the gut to the peripheral tissues: the multiple effects of butyrate. Nutrition Research Reviews 23, 366384.CrossRefGoogle Scholar
Hong, YH, Nishimura, Y, Hishikawa, D, Tsuzuki, H, Miyahara, H, Gotoh, C, Choi, KC, Feng, DD, Chen, C, Lee, HG, Katoh, K, Roh, SG and Sasaki, S 2005. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology 146, 50925099.Google Scholar
Karaki, S, Mitsui, R, Hayashi, H, Kato, I, Sugiya, H, Iwanaga, T, Furness, JB and Kuwahara, A 2006. Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine. Cell and Tissue Research 324, 353360.Google Scholar
Karaki, S, Tazoe, H, Hayashi, H, Kashiwabara, H, Tooyama, K, Suzuki, Y and Kuwahara, A 2008. Expression of the short-chain fatty acid receptor, GPR43, in the human colon. Journal of Molecular Histology 39, 135142.Google Scholar
Kim, JY, Cho, WJ, Kim, JH, Lim, SH, Kim, HJ, Lee, YW and Kwon, SW 2013. Efficacy and safety of hyaluronate membrane in the rabbit cecum-abdominal wall adhesion model. Journal of the Korean Surgical Society 85, 5157.Google Scholar
Kimura, I, Ozawa, K, Inoue, D, Imamura, T, Kimura, K, Maeda, T, Terasawa, K, Kashihara, D, Hirano, K, Tani, T, Takahashi, T, Miyauchi, S, Shioi, G, Inoue, H and Tsujimoto, G 2013. The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43. Nature Communications 4, 1829.Google Scholar
Kohles, M 2014. Gastrointestinal anatomy and physiology of select exotic companion mammals. Veterinary Clinics of North America: Exotic Animal Practice 17, 165178.Google ScholarPubMed
Le Poul, E, Loison, C, Struyf, S, Springael, JY, Lannoy, V, Decobecq, ME, Brezillon, S, Dupriez, V, Vassart, G, Van Damme, J, Parmentier, M and Detheux, M 2003. Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. The Journal of Biological Chemistry 278, 2548125489.CrossRefGoogle ScholarPubMed
Li, G, Su, H, Zhou, Z and Yao, W 2014. Identification of the porcine G protein-coupled receptor 41 and 43 genes and their expression pattern in different tissues and development stages. PloS One 9, e97342.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402408.Google Scholar
Mackie, RI, Sghir, A and Gaskins, HR 1999. Developmental microbial ecology of the neonatal gastrointestinal tract. The American Journal of Clinical Nutrition 69, 1035S1045S.Google Scholar
National Research Council (NRC) 1977. Nutrient requirements of rabbits, 2nd revised edition. National Academy of Sciences, Washington DC, USA.Google Scholar
Nilsson, NE, Kotarsky, K, Owman, C and Olde, B 2003. Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids. Biochemical and Biophysical Research Communications 303, 10471052.CrossRefGoogle ScholarPubMed
Nøhr, MK, Pedersen, MH, Gille, A, Egerod, KL, Engelstoft, MS, Husted, AS, Sichlau, RM, Grunddal, KV, Poulsen, SS, Han, S, Jones, RM, Offermanns, S and Schwartz, TW 2013. GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes. Endocrinology 154, 35523564.Google Scholar
Ogawa, K, Ben, RA, Pons, S, Paolo, MI and Fernández, LB 1992. Volatile fatty acids, lactic acid, and pH in the stools of breast-fed and bottle-fed infants. Journal of Pediatric Gastroenterology and Nutrition 15, 248252.Google Scholar
Scheppach, W and Weiler, F 2004. The butyrate story: old wine in new bottles? Current Opinion in Clinical Nutrition and Metabolic Care 7, 563567.Google Scholar
Stark, PL and Lee, A 1982. The bacterial colonization of the large bowel of pre-term low birth weight neonates. Journal of Hygiene (London) 89, 5967.Google Scholar
Stoddart, LA, Smith, NJ and Milligan, G 2008. International Union of Pharmacology. LXXI. Free fatty acid receptors FFA1,-2, and-3: pharmacology and pathophysiological functions. Pharmacological Reviews 60, 405417.Google Scholar
Tang, C, Ahmed, K, Gille, A, Lu, S, Gröne, HJ, Tunaru, S and Offermanns, S 2015. Loss of FFA2 and FFA3 increases insulin secretion and improves glucose tolerance in type 2 diabetes. Nature Medicine 21, 173177.Google Scholar
Tazoe, H, Otomo, Y, Karaki, S, Kato, I, Fukami, Y, Terasaki, M and Kuwahara, A 2009. Expression of short-chain fatty acid receptor GPR41 in the human colon. Biomedical Research 30, 149156.Google Scholar
Ulven, T 2012. Short-chain free fatty acid receptors FFAR2/GPR43 and FFAR3/GPR 41 as new potential therapeutic targets. Front Endocrinol (Lausanne) 3, 3389.Google Scholar
Wang, A, Gu, Z, Heid, B, Akers, RM and Jiang, H 2009. Identification and characterization of the bovine G protein-coupled receptor GPR41 and GPR43 genes. Journal of Dairy Science 92, 26962705.Google Scholar
Wong, JM, de Souza, R, Kendall, CW, Emam, A and Jenkins, DJ 2006. Colonic health: fermentation and short chain fatty acids. Journal of Clinical Gastroenterology 40, 235243.CrossRefGoogle ScholarPubMed
Xiong, Y, Miyamoto, N, Shibata, K, Valasek, MA, Motoike, T, Kedzierski, RM and Yanagisawa, M 2004. Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41. Proceedings of the National Academy of Sciences of the United States of America 101, 10451050.CrossRefGoogle ScholarPubMed
Supplementary material: File

Fu supplementary material

Tables S1-S2

Download Fu supplementary material(File)
File 32.8 KB