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Effect of breed on the expression of Sirtuins (Sirt1-7) and antioxidant capacity in porcine brain

Published online by Cambridge University Press:  08 October 2013

Y. Ren ,
T. Z. Shan ,
L. N. Zhu ,
T. Wu ,
J. Guo  and
Y. Z. Wang
Y. Ren
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
T. Z. Shan
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
L. N. Zhu
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
T. Wu
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
J. Guo
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
Y. Z. Wang*
Affiliation:
Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, People’s Republic of China
*
†E-mail: yzwang321@zju.edu.cn
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Abstract

Sirtuins, NAD-dependent histone deacetylase (HDAC), are correlated to aging and antioxidant. The aim of this study was to determine breed differences of porcine Sirtuins expression and antioxidant capacity in brain between Jinhua pigs (a fatty breed of China) and Danish Landrace pigs (a leaner breed). Effect of age on Sirtuins’ expression was also investigated. At the age of 180 days, the mRNA levels of Sirt1, as well as Sirt2 and Sirt4, in Jinhua pigs were greater, but the mRNA levels of Sirt3, Sirt5, Sirt6, and Sirt7 of Jinhua pigs were lower compared with Danish Landrace pigs. Likewise, at the same BW of 64 kg, the mRNA levels of Sirtuins, except Sirt5 and Sirt7, in Jinhua pigs were greater than Danish Landrace pigs. Meanwhile, Jinhua pigs possessed higher antioxidants activity than Danish Landrace pigs either at the same age or at the same BW. Furthermore, mRNA levels of Sirtuins were decreased with age in brain of the two breeds from 30 to 120 days. The results indicated that Sirtuins expression in brain was different between fatty and lean pigs, and Sirtuins expression may be correlated to antioxidant capacity. In addition, age could down-regulate Siruins expression in porcine brain.

Keywords

ageantioxidantbrainpigsSirtuins
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 12 , December 2013 , pp. 1994 - 1998
Copyright
Copyright © The Animal Consortium 2013 

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Footnotes

a

These authors contributed equally to this work.

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