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Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of Δ5- and Δ6-desaturase in tissues of female piglets

Published online by Cambridge University Press:  01 August 2009

J. Missotten ,
S. De Smet ,
K. Raes  and
O. Doran
J. Missotten
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
S. De Smet
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
K. Raes
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium Research Group EnBiChem, Department of Industrial Engineering and Technology, University College West-Flanders, Graaf Karel de Goedelaan 5, 8500 Kortrijk, Belgium
O. Doran*
Affiliation:
School of Life Sciences, University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, UK
*
†E-mail: olena.doran@uwe.ac.uk
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Abstract

The present study investigated whether enrichment of the pig maternal diet with n-3 polyunsaturated fatty acids (PUFA) affects the fatty-acid composition of female piglets via enhancing of expression of the lipogenic enzymes Δ5-desaturase (Δ5d) and Δ6-desaturase (Δ6d). The sows (50% Landrace × 50% Large White) were fed a control diet or one of the experimental diets starting at day 45 in gestation. The experimental diets were supplemented either with linseed oil or fish oil, whereas the control diet contained palm oil. Expression of Δ5d and Δ6d, and fatty-acid composition was determined by Western blotting and gas-liquid chromatography, respectively, in muscle, subcutaneous adipose tissue and liver. The highest Δ5d protein expression was observed in the piglets’ muscle, followed by subcutaneous adipose tissue, with the lowest level in the liver. Expression of Δ6d in the piglets’ tissues followed an opposite pattern, and was highest in the liver, followed by subcutaneous adipose tissue, with the lowest level in muscle. Supplementation of the maternal diet with fish oil or linseed oil increased the level of n-3 PUFA of the piglets in a tissue-specific manner. The response of Δ6d and Δ5d protein expression in female piglets, with average birth weight 2.4 kg, to the dietary manipulation was also tissue-specific. It is suggested that the increase in n-3 PUFA content in the progeny was related, at least partially, to the activation of Δ6d and Δ5d expression.

Keywords

pigdietfatty-acid compositionlipogenic enzymeprotein expression
Type
Full Paper
Information
animal , Volume 3 , Issue 8 , August 2009 , pp. 1196 - 1204
Copyright
Copyright © The Animal Consortium 2009

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References

Arbuckle, LD, Innis, SM 1993. Docosahexaenoic acid is transferred through maternal diet to milk and to tissues of natural milk-fed piglets. Journal of Nutrition 123, 1668–1675.CrossRefGoogle ScholarPubMed
Barcelo-Coblijn, G, Murphy, EJ, Othman, R, Moghadasian, MH, Kashour, T, Friel, JK 2008. Flaxseed oil and fish-oil capsule consumption alters human red blood cell n-3 fatty acid composition: a multiple-dosing trial comparing 2 sources of n-3 fatty acids. American Journal of Clinical Nutrition 88, 801–809.CrossRefGoogle Scholar
Berquin, IM, Edwards, IJ, Chen, YQ 2008. Multi-targeted therapy of cancer by omega-3 fatty acids. Cancer Letters 269, 363–377.CrossRefGoogle ScholarPubMed
Bradford, MM 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Brenner, RR 1974. The oxidative desaturation of unsaturated fatty acids in animals. Molecular and Cellular Biochemistry 3, 41–52.CrossRefGoogle ScholarPubMed
Cho, HP, Nakamura, M, Clarke, SD 1999a. Cloning, expression, and fatty acid regulation of the human Δ5-desaturase. Journal of Biological Chemistry 274, 37335–37339.CrossRefGoogle Scholar
Cho, HP, Nakamura, M, Clarke, SD 1999b. Cloning, expression and nutritional regulation of the mammalian Δ6-desaturase. Journal of Biological Chemistry 274, 471–477.CrossRefGoogle Scholar
Clarke, SD, Jump, D 1994. Dietary polyunsaturated fatty acid regulation of gene transcription. Annual Review of Nutrition 14, 83–98.CrossRefGoogle ScholarPubMed
Cleland, LG, James, MJ, Proudman, SM 2003. The role of fish oils in the treatment of rheumatoid arthritis. Drugs 63, 845–853.CrossRefGoogle ScholarPubMed
D’Arrigo, M, Hoz, L, Lopez-Bote, CJ, Cambero, I, Pin, C, Ordonez, JF 2002. Effect of dietary linseed oil on pig hepatic tissue fatty acid composition and susceptibility to lipid peroxidation. Nutrition Research 10, 1189–1196.CrossRefGoogle Scholar
Doran, O, Moule, SK, Teye, GA, Whittington, FM, Hallett, KG, Wood, JD 2006. A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: relationship with intramuscular lipid formation. British Journal of Nutrition 95, 609–617.CrossRefGoogle Scholar
Folch, J, Lees, M, Sloane-Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Fritsche, KL, Huang, S-C, Cassity, NA 1993. Enrichment of omega-3 fatty acids in suckling pigs by maternal dietary fish oil supplementation. Journal of Animal Science 71, 1841–1847.CrossRefGoogle ScholarPubMed
Garg, ML, Sebokova, E, Thomson, AB, Clandinin, MT 1988. Delta 6-desaturase activity in liver microsomes of rats fed diets enriched with cholesterol and/or omega 3 fatty acids. Biochemical Journal 249, 351–356.CrossRefGoogle ScholarPubMed
Girard, J, Perdereau, D, Foufelle, F, Prip-Buus, C, Ferre, P 1994. Regulation of lipogenic enzyme gene expression by nutrients and hormones. The FASEB Journal 8, 36–42.CrossRefGoogle ScholarPubMed
Givens, ID, Gibbs, RA 2008. Current intakes of EPA and DHA in European populations and the potential of animal-derived foods to increase them. Proceedings of Nutrition Society 67, 273–280.CrossRefGoogle Scholar
Gondret, F, Ferre, P, Dugail, I 2001. ADD-1/SREBP-1 is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. Journal of Lipid Research 42, 106–113.CrossRefGoogle Scholar
Iritany, N, Fukusa, H, Tada, K 1996. Nutritional regulation of lipogenic enzyme gene expression in rat epididymal adipose tissue. Journal of Biochemistry 120, 242–248.CrossRefGoogle Scholar
ISO 6492 1999. International Organization for Standardization. Animal feeding stuffs. Determination of fat content. ISO, Geneve, Switzerland.Google Scholar
ISO 5984 2002. International Organization for Standardization. Animal feeding stuffs. Determination of crude ash. ISO, Geneve, Switzerland.Google Scholar
ISO 5983-1 2005. International Organization for Standardization. Animal feeding stuffs. Determination of nitrogen content and calculation of crude protein content. Part 1: Kjeldahl method. ISO, Geneve, Switzerland.Google Scholar
Lauridsen, C, Jensen, SK 2007. Lipid composition of lactational diets influences the fatty acid profile of the progeny before and after suckling. Animal 1, 952–962.CrossRefGoogle ScholarPubMed
Leaf, A, Xiao, YF, Kang, JX, Billman, GE 2003. Prevention of sudden cardiac death by n-3 polyunsaturated fatty acids. Pharmacology and Therapeutics 98, 355–377.CrossRefGoogle ScholarPubMed
Leskanich, CO, Noble, RC 1999. The comparative roles of polyunsaturated fatty acids in pig neonatal development. British Journal of Nutrition 81, 87–106.CrossRefGoogle ScholarPubMed
Li, JJ, Huang, CJ, Xie, D 2008. Anti-obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid. Molecular Nutrition and Food Research 52, 631–645.CrossRefGoogle ScholarPubMed
Matsuzaka, T, Shimano, H, Yahagi, N, Amemiya-Kudo, M, Yoshikawa, T, Hasty, AH, Tamura, Y, Osuga, JI, Okazaki, H, Iizuka, Y, Takahashi, A, Sone, H, Gotoda, T, Ishibashi, S, Yamada, N 2002. Dual regulation of mouse Δ5- and Δ6-desaturase gene expression by SREBP-1 and PPARα. Journal of Lipid Research 43, 107–114.CrossRefGoogle Scholar
Moghadasian, MH 2008. Advances in dietary enrichment with n-3 fatty acids. Critical Reviews in Food Science and Nutrition 48, 402–410.CrossRefGoogle ScholarPubMed
Mourot, J, Hermier, D 2001. Lipids in monogastric meat (review article). Reproduction Nutrition Development 41, 109–118.CrossRefGoogle Scholar
Mozaffarian, D 2008. Fish and n-3 fatty acids for the prevention of fatal coronary heart disease and sudden cardiac death. American Journal of Clinical Nutrition 87, 1991S–1996S.CrossRefGoogle ScholarPubMed
Neuringer, M, Anderson, GJ, Connor, WE 1988. The essentiality of n-3 fatty acids for the development and function of the retina and brain. Annual Review of Nutrition 8, 517–541.CrossRefGoogle ScholarPubMed
Nuernberg, K, Fischer, K, Nuernber, G, Kuechenmeister, U, Klosowska, D, Eliminowska-Wenda, G, Fiedler, I, Ender, K 2005. Effects of dietary olive and linseed oil on lipid composition, meat quality, sensory characteristics and muscle structure in pigs. Meat Science 70, 63–64.CrossRefGoogle ScholarPubMed
Raes, K, De Smet, S, Demeyer, D 2001. Effect of double-muscling in Belgian Blue bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids. Animal Science 73, 253–260.CrossRefGoogle Scholar
Ramsay, TG, Karousis, J, White, ME, Wolverton, CK 1991. Fatty acid metabolism by the porcine placenta. Journal of Animal Science 69, 3645–3654.CrossRefGoogle ScholarPubMed
Rigau, AP, Lindemann, MD, Kornegay, ET, Harper, AF, Watkins, BA 1995. Role of dietary lipids on fetal tissue fatty acid composition and fetal survival in swine at 42 days of gestation. Journal of Animal Science 73, 1372–1380.CrossRefGoogle Scholar
Rooke, JA, Bland, IM, Edwards, SA 1998. Effect of feeding tuna oil or soyabean oil as supplements to sows in late pregnancy on piglet tissue composition and viability. British Journal of Nutrition 80, 273–280.CrossRefGoogle ScholarPubMed
Rooke, JA, Sinclair, AG, Edwards, SA, Cordoba, R, Pkiyach, S, Penny, PC, Penny, P, Finch, AM, Horgan, GW 2001. The effect of feeding salmon oil to sows throughout pregnancy on pre-weaning mortality of piglets. Animal Science 73, 489–500.CrossRefGoogle Scholar
Ruxton, CH, Reed, SC, Simpson, MJ, Millington, KJ 2004. The health benefit of omega-3 polyunsaturated fatty acids: a review of the evidence. Journal of Human Nutrition and Dietetics 17, 449–459.CrossRefGoogle Scholar
SanGiovanni, JP, Chew, EY, Agron, E, Clemons, TE, Ferris, FL 3rd, Gensler, G, Lindblad, AS, Milton, RC, Seddon, JM, Klein, R, Sperduto, RD 2008. The relationship of dietary omega-3 long-chain polyunsaturated fatty acid intake with incident age-related macular degeneration: AREDS report no. 23. Archives of Ophthalmology 126, 1274–1279.CrossRefGoogle ScholarPubMed
Shimomura, I, Shimano, H, Horton, JD, Goldstein, JL, Brown, MS 1997. Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. The Journal of Clinical Investigation 99, 838–845.CrossRefGoogle ScholarPubMed
Sprecher, H 2000. Metabolism of highly unsaturated n-3 and n-6 fatty acids. Biochimica et Biophysica Acta 1486, 219–231.CrossRefGoogle ScholarPubMed
Thulin, AJ, Allee, GL, Harmon, DL, Davis, DL 1989. Utero-placenta transfer of octanoic, palmitic and linoleic acids during late gestation in gilts. Journal of Animal Science 67, 738–745.CrossRefGoogle Scholar
Vanschoonbeek, K, de Maat, MP, Heemskerk, JW 2003. Fish oil consumption and reduction of arterial disease. The Journal of Nutrition 133, 657–660.CrossRefGoogle ScholarPubMed
Wang, Y, Botolin, D, Xu, J, Christian, B, Mitchell, E, Jayaprakasam, B, Nair, M, Peters, JM, Busik, J, Olson, LK, Jump, DB 2006. Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity. Journal of Lipid Research 47, 2028–2034.CrossRefGoogle ScholarPubMed
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI, Whittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.CrossRefGoogle ScholarPubMed
Xiang, M, Rahman, MA, Ai, H, Li, X, Harbige, LS 2006. Diet and gene expression: delta-5 and delta-6 desaturases in healthy Chinese and European subjects. Annals of Nutrition and Metabolism 50, 492–498.CrossRefGoogle ScholarPubMed