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Effects of intravenous arginine infusion on inflammation and metabolic indices of dairy cows in early lactation

Published online by Cambridge University Press:  01 October 2019

L. Y. Ding Open the ORCID record for L. Y. Ding [Opens in a new window] ,
Y. F. Wang ,
Y. Z. Shen ,
G. Zhou ,
T. Y. Wu ,
X. Zhang ,
M. Z. Wang ,
J. J. Loor  and
J. Zhang
L. Y. Ding
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China Faculty of Science, School of Agriculture and Environment, The University of Western Australia, 35 Stirling Highway, Perth 6009, WA, Australia
Y. F. Wang
Affiliation:
Faculty of Physiology, Clinical Medical School, Southeast University, 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu, P.R. China
Y. Z. Shen
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
G. Zhou
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
T. Y. Wu
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
X. Zhang
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
M. Z. Wang*
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
J. J. Loor
Affiliation:
Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana 61801, IL, USA
J. Zhang
Affiliation:
Department of Animal Nutrition, College of Animal Science and Technology, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, P.R. China
*
†E-mail: mengzhiwang@126.com
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Abstract

Enhancing the supply of arginine (Arg), a semi-essential amino acid, has positive effects on immune function in dairy cattle experiencing metabolic stress during early lactation. Our objective was to determine the effects of Arg supplementation on biomarkers of liver damage and inflammation in cows during early lactation. Six Chinese Holstein lactating cows with similar BW (508 ± 14 kg), body condition score (3.0), parity (4.0 ± 0), milk yield (30.6 ± 1.8 kg) and days in milk (20 ± days) were randomly assigned to three treatments in a replicated 3 × 3 Latin square design balanced for carryover effects. Each period was 21 days with 7 days for infusion and 14 days for washout. Treatments were (1) Control: saline; (2) Arg group: saline + 0.216 mol/day l-Arg; and (3) Alanine (Ala) group: saline + 0.868 mol/day l-Ala (iso-nitrogenous to the Arg group). Blood and milk samples from the experimental cows were collected on the last day of each infusion period and analyzed for indices of liver damage and inflammation, and the count and composition of somatic cells in milk. Compared with the Control, the infusion of Arg led to greater concentrations of total protein, immunoglobulin M and high density lipoprotein cholesterol coupled with lower concentrations of haptoglobin and tumor necrosis factor-α, and activity of aspartate aminotransferase in serum. Infusion of Ala had no effect on those biomarkers compared with the Control. Although milk somatic cell count was not affected, the concentration of granulocytes was lower in response to Arg infusion compared with the Control or Ala group. Overall, the biomarker analyses indicated that the supplementation of Arg via the jugular vein during early lactation alleviated inflammation and metabolic stress.

Keywords

amino acidshealthsomatic cell compositionmetabolic stressimmune function
Type
Research Article
Information
animal , Volume 14 , Issue 2 , February 2020 , pp. 346 - 352
Copyright
© The Animal Consortium 2019 

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Footnotes

a

Present address: Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana 61801, IL, USA

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