Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T04:28:05.278Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of inulin and di-d-fructose dianhydride-enriched caramels on intestinal microbiota composition and performance of broiler chickens

Published online by Cambridge University Press:  10 September 2013

M. J. Peinado ,
A. Echávarri ,
R. Ruiz ,
E. Suárez-Pereira ,
C. Ortiz Mellet ,
J. M. García Fernández  and
L. A. Rubio
M. J. Peinado
Affiliation:
Depto. de Fisiología y Bioquímica de la Nutrición Animal (INAN, EEZ, CSIC), Profesor Albareda, 1, 18008 Granada, Spain
A. Echávarri
Affiliation:
Depto. de Fisiología y Bioquímica de la Nutrición Animal (INAN, EEZ, CSIC), Profesor Albareda, 1, 18008 Granada, Spain
R. Ruiz
Affiliation:
Depto. de Fisiología y Bioquímica de la Nutrición Animal (INAN, EEZ, CSIC), Profesor Albareda, 1, 18008 Granada, Spain
E. Suárez-Pereira
Affiliation:
Depto. de Química Orgánica, Facultad de Química, Univ. de Sevilla, Apdo. 1203, E-41071 Sevilla, Spain
C. Ortiz Mellet
Affiliation:
Depto. de Química Orgánica, Facultad de Química, Univ. de Sevilla, Apdo. 1203, E-41071 Sevilla, Spain
J. M. García Fernández
Affiliation:
Instituto de Investigaciones Químicas (IIQ) CSIC and Univ. de Sevilla, Américo Vespucio 49, Isla de la Cartuja, E-41092 Sevilla, Spain
L. A. Rubio*
Affiliation:
Depto. de Fisiología y Bioquímica de la Nutrición Animal (INAN, EEZ, CSIC), Profesor Albareda, 1, 18008 Granada, Spain
*
†E-mail: lrubio@eez.csic.es
Get access

Abstract

In vitro and in vivo experiments were designed to evaluate the effectiveness of laboratory-made di-d-fructose dianhydride (DFA)-enriched caramels. The DFA-enriched caramels were obtained from d-fructose (FC), d-fructose and sucrose (FSC), or d-fructose and β-cyclodextrin (FCDC). In the in vitro experiment, raftilose and all caramels increased (P<0.05) l-lactate concentration and decreased (P<0.05) pH. Total short-chain fatty acid concentration was higher (P<0.05) than controls in tubes containing raftilose, FSC, FCDC and commercial sucrose caramel (CSC). Raftilose, and all caramels tested except FSC and FC (1%), increased (P<0.01) lactobacilli log10 number of copies compared with the non-additive control. FSC, FCDC and CSC increased (P<0.01) the bifidobacteria number of copies as compared with controls. All additives, except FCDC, decreased (P<0.01) Clostridium coccoides/Eubacterium rectale log number of copies. Compared with controls, raftilose, FC and CSC led to lower (P<0.01) Escherichia–Shigella and enterobacteria. For the in vivo experiment, a total of 144 male 1-day-old broiler chickens of the Cobb strain were randomly assigned to one of the three dietary treatments for 21 days. Dietary treatments were control (commercial diet with no additive), inulin (20 g inulin/kg diet) and FC (20 g FC/kg diet). Final BW of birds fed FC diet was higher (P<0.01) than controls or inulin-fed birds, although feed: gain values were not different. Feed intake of chickens fed FC was higher (P<0.01) than that of inulin-fed birds but not statistically different from controls. Crop pH values were lower (P<0.01) in birds fed FC diet as compared with control diet, with inulin-fed chickens showing values not different from control- or FC-fed birds. Lower (P<0.05) lactobacilli number of copies was determined in the crop, ileum and caeca of birds fed the inulin diet compared with the control diet. Inulin supplementation also resulted in lower (P<0.05) C. coccoides/E. rectale, bacteroides and total bacteria in caecal contents. Addition of FC to broiler diets gave place to lower (P<0.05) enterobacteria and Escherichia–Shigella in crop and caecal contents compared with controls. The bacteroides number of copies increased (P<0.05) as compared with controls in the ileum, but decreased (P<0.05) in the caeca of chickens fed the FC diet. Energy, ADF, NDF and non-starch polysaccharides faecal digestibilities were greater (P<0.05) than controls in chickens fed diets containing inulin or FC. Fat digestibility was higher (P<0.05) in FC-fed birds compared with controls or inulin-fed chickens. In conclusion, DFA-enriched caramels tested here, particularly FC, may represent a type of new additives useful in poultry production.

Keywords

broilerdi-d-fructose dianhydridesinulinmicrobiotaprebiotics
Type
Nutrition
Information
animal , Volume 7 , Issue 11 , November 2013 , pp. 1779 - 1788
Copyright
Copyright © The Animal Consortium 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Annison, G 1991. Relationship between the levels of soluble non-starch polysaccharides and the apparent metabolisable energy of wheats assayed in broiler chickens. Journal of Agricultural and Food Chemistry 39, 1252–1256.CrossRefGoogle Scholar
Arribas, B, Suárez-Pereira, E, Ortiz Mellet, C, García Fernández, JM, Buttersack, C, Rodríguez-Cabezas, ME, Garrido-Mesa, N, Bailon, E, Guerra-Hernández, E, Zarzuelo, A and Gálvez, J 2010. Di-d-fructose-enriched caramels: effect on colon microbiota, inflammation, and tissue damage in trinitrobenzenesulfonic acid-induced colitic rats. Journal of Agricultural and Food Chemistry 58, 6476–6484.Google Scholar
Bourlinoux, P, Koletzko, B and Guarner, FV 2003. The intestine and its microflora are partners for the protection of the host. American Journal of Clinical Nutrition 78, 675–683.Google Scholar
Bry, L, Falk, PG, Midtvedt, T and Gordon, JI 1996. A model of host-microbial interactions in an open mammalian ecosystem. Science 273, 1380–1383.Google Scholar
Candela, M, Maccaferri, S, Turoni, S, Carnevali, P and Brigidi, P 2010. Functional intestinal microbiome, new frontiers in prebiotic design. International Journal of Food Microbiology 140, 93–101.Google Scholar
Choct, M, Hughes, RJ, Wang, J, Bedford, MR, Morgan, AJ, Annison, G 1996. Increased small intestinal fermentation responsible for the anti-nutritive activity of non-starch polysaccharides in chickens. British Poultry Science 37, 609–621.CrossRefGoogle ScholarPubMed
EC 2003. Commission of the European Communities, Commission Regulation (EC) No. 1831/2003. Official Journal of European Union I 268, 29–43.Google Scholar
Englyst, HN, Quigley, ME, Hudson, GJ and Cummings, JH 1982. Determination of dietary fiber as non-starch polysaccharides by gas-liquid chromatography. Analyst 117, 1707–1714.CrossRefGoogle Scholar
Fenton, TW and Fenton, M 1979. An improved procedure for the determination of chromic oxide in feed and faeces. Canadian Journal of Animal Science 59, 631–634.Google Scholar
Fonseca, BB, Beletti, ME, da Silva, MS, da Silva, PL, Duarte, IN and Rossi, DA 2010. Microbiota of the cecum, ileum morphology, pH of the crop and performance of broiler chickens supplemented with probiotics. Revista Brasileira de Zootecnia 39, 1756–1760.CrossRefGoogle Scholar
Gaggìa, F, Mattarelli, P and Biavati, B 2010. Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology 141, S15–S28.Google Scholar
Geier, MS, Torok, VA, Allison, GE, Ophel-Keller, K and Hughes, RJ 2009. Indigestible carbohydrates alter the intestinal microbiota but do not influence the performance of broiler chickens. Journal of Applied Microbiology 106, 1540–1548.CrossRefGoogle Scholar
Hill, FW and Anderson, DL 1958. Comparison of metabolizable energy and productive energy determinations with growing chicks. Journal of Nutrition 64, 587–603.CrossRefGoogle ScholarPubMed
Huyghebaert, G, Ducatelle, R and Van Immerseel, F 2011. An update on alternatives to antimicrobial growth promoters for broilers. Veterinary Journal 187, 182–188.Google Scholar
Kim, G-B, Seo, JM, Kim, CH and Paik, IK 2011. Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poultry Science 90, 75–82.Google Scholar
Langhout, DL, Schutte, JB, Geerse, C, Kies, AK, De Jong, J and Verstegen, MWA 1997. Effects on chick performance and nutrient digestibility of an endo‐xylanase added to a wheat‐ and rye‐based diet in relation to fat source. British Poultry Science 38, 557–563.Google Scholar
Li, XJ, Piao, XS, Kim, SW, Liu, P, Wang, L, Shen, YB, Jung, SC and Lee, HS 2007. Effects of chito-oligosaccharide supplementation on performance, nutrient digestibility, and serum composition in broiler chickens. Poultry Science 86, 1107–1114.Google Scholar
Malayoğlu, HB, Baysal, S, Misirlioğlu, Z, Polat, M, Yilmaz, H and Turan, N 2010. Effects of oregano essential oil with or without feed enzymes on growth performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat-soybean meal diets. British Poultry Science 51, 67–80.CrossRefGoogle Scholar
Manley-Harris, M and Richards, GN 1997. Dihexulose dianhydrides. Advances in Carbohydrate Chemistry and Biochemistry 52, 207–239.Google Scholar
Orban, JI, Patterson, JA, Sutton, AL and Richards, GN 1997. Effect of sucrose thermal oligosaccharide caramel, dietary vitamin-mineral level, and brooding temperature on growth and intestinal bacterial populations of broiler chickens. Poultry Science 76, 482–490.Google Scholar
Ortiz Mellet, C and García Fernández, JM 2010. Difructose dianhydrides (DFAs) and DFA enriched products as functional foods. Topics in Current Chemistry 294, 49–77.Google Scholar
Patterson, JA and Burkholder, KM 2003. Application of prebiotics and probiotics in poultry production. Poultry Science 82, 627–631.Google Scholar
Peinado, MJ, Ruiz, R, Echávarri, A and Rubio, LA 2012. Garlic derivative PTS-O is effective against broiler pathogens in vivo. Poultry Science 91, 2148–2157.Google Scholar
Pelicano, ERL, Souza, PA, Souza, HBA, Figueiredo, DF, Boiago, MM, Carvalho, SR and Bordon, BF 2005. Intestinal mucosa development in broiler chickens fed natural growth promoters. Brazilian Journal of Poultry Science 7, 221–229.Google Scholar
Phillips, ML 2009. Gut reaction: environmental effects on the human microbiota. Environmental and Health Perspectives 117, A198–A205.CrossRefGoogle ScholarPubMed
Playne, MJ 1985. Determination of ethanol, volatile fatty acids, lactic acid and succine acids in fermentation liquids by gas chromatography. Journal of the Science of Food and Agriculture 36, 638–643.Google Scholar
Rebolé, A, Ortiz, LT, Rodríguez, ML, Alzueta, C, Treviño, J and Velasco, S 2010. Effects of inulin and enzyme complex, individually or in combination, on growth performance, intestinal microflora, cecal fermentation characteristics, and jejunal histomorphology in broiler chickens fed a wheat- and barley-based diet. Poultry Science 89, 276–286.Google Scholar
Roberfroid, M 2007. Prebiotics: the concept revisited. Journal of Nutrition 137, 830S–837S.Google Scholar
Rose, DJ, Venema, K, Keshavarzian, A and Hamaker, BR 2010. Starch-entrapped microspheres show a beneficial fermentation profile and decrease in potentially harmful bacteria during in vitro fermentation in faecal microbiota obtained from patients with inflammatory bowel disease. British Journal of Nutrition 103, 1514–1524.Google Scholar
Ruiz, R and Rubio, LA 2009. Lyophyllization improves the extraction of PCR-quality community DNA from pig faecal samples. Journal of the Science of Food and Agriculture 89, 723–727.Google Scholar
Ruiz, R, García, MP, Lara, A and Rubio, LA 2010. Garlic derivatives (PTS and PTS-O) differently affect the ecology of swine faecal microbiota in vitro. Veterinary Microbiology 144, 110–117.Google Scholar
Suárez-Pereira, E, Rubio, EM, Pilard, S, Ortiz Mellet, C and García-Fernández, JM 2010. Di-d-fructose dianhydride (DFA)-enriched caramels by acid ion-exchange resin-promoted caramelization of d-fructose: chemical analysis. Journal of Agricultural and Food Chemistry 58, 1777–1787.Google Scholar
Torok, VA, Allison, GE, Percy, NJ, Ophel-Keller, K and Hughes, RJ 2011a. Influence of antimicrobial feed additives on broiler commensal posthatch gut microbiota development and performance. Applied and Environmental Microbiology 77, 3380–3390.CrossRefGoogle ScholarPubMed
Torok, VA, Hughes, RJ, Mikkelsen, LL, Pérez-Maldonado, RP, Balding, K, MacAlpine, R, Percy, NJ and Ophel-Keller, K 2011b. Identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials. Applied and Environmental Microbiology 77, 5868–5878.Google Scholar
Tzortzis, G, Goulas, AK, Gee, JM and Gibson, GR 2005. A novel galacto-oligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. Journal of Nutrition 135, 1726–1731.CrossRefGoogle Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle Scholar
Williams, BA, Verstegen, MW and Tamminga, S 2001. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutrition Research Reviews 14, 207–228.Google Scholar
Supplementary material: File

Peinado Supplementary Material

Supplementary Material

Download Peinado Supplementary Material(File)
File 33.8 KB