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A grass-based diet favours muscle n-3 long-chain PUFA deposition without modifying gene expression of proteins involved in their synthesis or uptake in Charolais steers

Published online by Cambridge University Press:  06 August 2013

M. Cherfaoui ,
D. Durand ,
M. Bonnet ,
L. Bernard ,
D. Bauchart ,
I. Ortigues-Marty  and
D. Gruffat
M. Cherfaoui*
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
D. Durand
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
M. Bonnet
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
L. Bernard
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
D. Bauchart
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
I. Ortigues-Marty
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
D. Gruffat
Affiliation:
INRA, UMR 1213 Herbivore, F-63122 Saint-Genès-Champanelle, France
*
†E-mail: dominique.gruffat@clermont.inra.fr
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Abstract

N-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) are subject of growing interest as they are of particular relevance for meat quality and human health. However, their content in the muscles of cattle is generally low probably as the complex result of their biosynthesis from dietary n-3 PUFA in the muscle and/or in other tissues/organs and of their subsequent uptake by the muscle. In view of this, this study aimed at understanding whether the changes in the muscle n-3 LCPUFA content, depending on the diet (maize silage v. grass) or the muscle type (Rectus abdominis, RA v.Semitendinosus, ST) in 12 Charolais steers, were related to variations in the gene expression of proteins involved in n-3 LCPUFA biosynthesis or cellular uptake. Tissue fatty acid composition was analysed by gas-liquid chromatography and mRNA abundance of proteins by quantitative real-time PCR. The grass-based diet resulted in a 2.3-fold (P < 0.0002) increase in both RA and ST n-3 LCPUFA content compared with the maize silage-based diet, whereas no difference in the expression of genes involved in n-3 LCPUFA biosynthesis and uptake was observed between diets. ST exhibited a 1.5-fold higher n-3 LCPUFA content than RA (P < 0.003), whereas the gene expression of proteins involved in n-3 LCPUFA biosynthesis and uptake was 1.3- to 18-fold higher in RA than in ST (P < 0.05). In conclusion, diet- or muscle type-dependent changes in the muscle n-3 LCPUFA content of Charolais steers did not seem to be mediated by the gene expression regulation of proteins involved in the biosynthesis or uptake of these fatty acids.

Keywords

n-3 LCPUFA, synthesis, uptake, steer muscles, grass
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 11 , November 2013 , pp. 1833 - 1840
Copyright
Copyright © The Animal Consortium 2013 

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Table S2

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