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Impacts of CLA and dietary concentrate proportion on blood metabolite concentration and proliferation of peripheral blood mononuclear cells of periparturient dairy cows

Published online by Cambridge University Press:  10 November 2014

M. Petzold ,
U. Meyer ,
S. Kersten ,
J. Spilke ,
G. Breves  and
S. Dänicke
M. Petzold
Affiliation:
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany
U. Meyer*
Affiliation:
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany
S. Kersten
Affiliation:
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany
J. Spilke
Affiliation:
Institute of Agricultural and Nutritional Sciences, Biometrics and Informatics in Agriculture Group, Martin-Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
G. Breves
Affiliation:
Department of Physiology, University of Veterinary Medicine, 30173 Hannover, Germany
S. Dänicke
Affiliation:
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany
*
†E-mail: Ulrich.Meyer@fli.bund.de
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Abstract

The study aimed to examine effects of supplemented CLA to periparturient dairy cows receiving different concentrate proportions antepartum (a.p.) to investigate CLA effects on metabolism and immune function. Compared with adapted feeding, high-concentrate diet a.p. should induce a ketogenic metabolic situation postpartum (p.p.) to better understand how CLA works. A total of 64 pregnant German Holstein cows had ad libitum access to partial mixed rations based on concentrate and roughage 3 weeks before calving until day 60 p.p. A.p., cows received 100 g/day control fat (CON) or a CLA supplement, either in a low-concentrate (20%, CON-20, CLA-20) or high-concentrate diet (60%, CON-60, CLA-60). P.p., concentrate proportion was adjusted to 50% while fat supplementation continued. After day 32 p.p., half of the animals of CLA-groups changed to CON supplementation (CLA-20-CON, CLA-60-CON). A ketogenic metabolic state p.p. was not achieved and respective impacts of CLA could not be examined. Blood samples for isolation of peripheral blood mononuclear cells (PBMC) were collected on day −21, 7, 28 and 56 relative to calving. Blood chemistry samples were taken over the entire experimental period. Mitogen-stimulated proliferation of PBMC remained unaffected. Besides serum concentrations of triglycerides, total bilirubin, total protein, albumin and IGF-1, clinical-chemical serum characteristics remained uninfluenced by treatments. No post-supplementation effect could be observed. Measured blood metabolites and mitogen-stimulated proliferation of PBMC indicate that all groups had an increased metabolic stress around calving, whereby group CLA-20 was affected more severely. Overall, supplemented CLA did not positively affect metabolism or immune function of periparturient dairy cows. However, feeding CLA in a low-concentrate diet a.p. seems to increase liver stress around calving via reduced DMI.

Keywords

CLAconcentratescowsblood metabolitesperipheral blood mononuclear cells
Type
Research Article
Information
animal , Volume 9 , Issue 3 , March 2015 , pp. 481 - 489
Copyright
© The Animal Consortium 2014 

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