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Nitric oxide concentrations in mammary quarters during heifer mastitis*

Published online by Cambridge University Press:  19 October 2012

A. Bastan ,
M. Cengiz ,
S. Cengiz ,
T. Sel ,
B. Polat ,
A. Colak ,
M. Akan  and
I. Darbaz
A. Bastan
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
M. Cengiz*
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ataturk University, 25240 Erzurum, Turkey
S. Cengiz
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25240 Erzurum, Turkey
T. Sel
Affiliation:
Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
B. Polat
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ataturk University, 25240 Erzurum, Turkey
A. Colak
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ataturk University, 25240 Erzurum, Turkey
M. Akan
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
I. Darbaz
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
*
†E-mail: mecevet@hotmail.com
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Abstract

The aim of this study was to evaluate nitric oxide (NOx) concentration in infected and non-infected mammary quarters of dairy heifers before and after calving. The relationship between bacterial species and NOx concentrations, as well as correlation between NOx concentrations and postpartum somatic cell count (SCC), was assessed. Coagulase-negative staphylococci, Staphylococcus aureus and Escherichia coli were the bacteria commonly isolated during the pre- and postpartum period. Infected quarters had greater NOx concentrations than non-infected quarters before (30.81 v. 22.83 μM/ml, P < 0.05) and after (9.56 v. 5.77 μM/ml, P < 0.0001) calving. It was determined that the interaction between sampling period and infectious status had no effect on NOx concentration (P < 0.16). Infected quarters had greater SCC (log10) than healthy quarters (4.95 v. 4.39; P < 0.0001). NOx concentrations, however, did not correlate with SCC (r = 0.02). In summary, changes in NOx concentration were mainly dependent on the infectious status of the quarters with variations among the bacterial species (P < 0.05).

Keywords

heifermastitisnitric oxidesomatic cell count
Type
Behaviour, welfare and health
Information
animal , Volume 7 , Issue 3 , March 2013 , pp. 499 - 504
Copyright
Copyright © The Animal Consortium 2012

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Footnotes

*

This study was supported by the Scientific and Technological Research Council of Turkey: grant no. 1080319

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