Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-22T21:56:18.909Z Has data issue: false hasContentIssue false
  • English
  • Français

Semen quality, testosterone, seminal plasma biochemical and antioxidant profiles of rabbit bucks fed diets supplemented with different concentrations of soybean lecithin

Published online by Cambridge University Press:  23 November 2011

Y. A. Attia  and
K. I. Kamel
Y. A. Attia*
Affiliation:
Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
K. I. Kamel
Affiliation:
Waterfowl and Rabbit Breeding Department, Animal production Research Institute, Agricultural Research Center, P. O. Box 12618, Dokki, Giza, Egypt
*
†E-mail: yfat_alexu40@hotmail.com
Get access

Abstract

A total of 28 adult V-line rabbits were fed ad libitum a control diet or a diet supplemented with 0.5%, 1.0% and 1.5% soybean lecithin (SL) for 12 weeks. Bucks that received 0.5%, 1.0% or 1.5% dietary SL had a higher ejaculate volume, mass motility, sperm concentration, total sperm output and total motile sperm. Dietary SL reduced the percentage of dead sperm and increased the normal sperm, and this concurred with an increase in blood testosterone concentration. Blood and seminal plasma total lipid, acid phosphatase and seminal plasma alkaline phosphatase were significantly increased because of inclusion of SL. Interestingly, SL reduced blood and seminal plasma thiobarbituric acid-reactive substances while increasing blood and seminal plasma glutathione content, glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity. Conception rate and litter size at birth and weaning were also significantly improved. Practically, it could be suggested that SL is a suitable supplement for improving semen quality, antioxidant status, reproductive traits and the economic efficiency of V-line rabbit bucks and 1% is an adequate concentration.

Keywords

lecithinrabbitssemen qualityantioxidant enzymes
Type
Full Paper
Information
animal , Volume 6 , Special Issue 5: Symposium “Sustainable animal production in the tropics” (SAPT), Guadeloupe, France, November 15–18, 2010 , May 2012 , pp. 824 - 833
Copyright
Copyright © The Animal Consortium 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aabdallah, DM, Eid, NI 2004. Possible neuroprotective effects of lecithin and alpha-tocopherol alone or in combination against ischemia/reperfusion insult in rat brain. Journal of Biochemistry Molecular Toxicology 18, 273–278.CrossRefGoogle ScholarPubMed
Aitken, RJ, Baker, HW 1995. Seminal leukocytes: passengers, terrorists or good Samaritans. Human Reproduction 10, 1736–1739.CrossRefGoogle ScholarPubMed
Al-Daraji, HJ, Al-Hassani, DH, Al-Tikriti, BTO, Abd-Alabaas, MH 2001. The influence of breed and season on semen quality of cocks. IPA. Journal Agriculture Research 11, 152–162.Google Scholar
Al-Daraji, HJ, Al-Mashadani, HA, Al-Hayani, WK, Al-Hassani, AS, Mirza, HA 2010. Effect of n-3 and n-6 fatty acid supplemented diets on semen quality in Japanese quail (Coturnix coturnix japonica). International Journal of Poultry Science 9, 656–663.CrossRefGoogle Scholar
Alvarez, JG, Storey, BT 1989. Role of glutathione-peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid-peroxidation. Gamete Research 23, 77–90.CrossRefGoogle ScholarPubMed
Attia, YA, Burke, WH, Yamani, KA, Jensen, LS 1993. Energy allotments and performance of broiler breeders. Poultry Science 72, 42–50.CrossRefGoogle Scholar
Attia, YA, Burke, WH, Yamani, KA, Jensen, LS 1995. Energy allotments and performance of broiler breeders. 1. Males. Poultry Science 74, 247–260.CrossRefGoogle ScholarPubMed
Attia, YA, Hussein, AS, Tag El-Din, AE, Qota, EM, Abed El-Ghany, AI, El-Sudany, AM 2009a. Improving productive and reproductive performance of dual-purpose crossbred hens in the tropics by lecithin supplementation. Tropical Animal Health Production 41, 461–475.CrossRefGoogle ScholarPubMed
Attia, YA, Al-Hanoun, A, Bovera, F 2009b. Effect of different levels of bee pollen on performance and blood profile of New Zealand White bucks and growth performance of their offspring during summer and winter months. Journal of Animal Physiology and Animal Nutrition 95, 17–26.CrossRefGoogle Scholar
Beutler, E, Duron, O, Kelly, BM 1963. An improved method for the detection of blood glutathione. Journal of Laboratory and Clinical Medicine 61, 882–888.Google Scholar
Biosource-Europe S.A. 8, rue de L'lndustrie.B-1400 Nivelles, Belgium.Google Scholar
Blom, E 1950. A one-minute live–dead sperm stain by means of eosin–nigrosin. Journal Fertility and Sterility 1, 176–177.CrossRefGoogle Scholar
Bongalhardo, DC, Leeson, S, Buhr, MM 2009. Dietary lipids differentially affect membranes from different areas of rooster sperm. Poultry Science 88, 1060–1069.CrossRefGoogle ScholarPubMed
Brun, JM, Theau-Clément, M, Bolet, G 2002. The relationship between rabbit semen characteristics and reproductive performance after artificial insemination. Animal Reproduction Science 70, 139–149.CrossRefGoogle ScholarPubMed
Carreau, S, Silandre, D, Bois, C, Bouraima, H, Galeraud-Denis, I, Delalande, C 2007. Estrogens: a new player in spermatogenesis. Folia Histochemica et Cytobiologica 45 (suppl. 1), S5–S10.Google ScholarPubMed
Castellini, C, Cardinali, R, Dal Bosco, A, Minelli, A, Camici, O 2006. Lipid composition of the main fractions of rabbit semen. Theriogenology 65, 703–712.CrossRefGoogle ScholarPubMed
Castellini, C, Lattaioli, P, Bernardini, M, Dal Bosco, A 2000. Effect of dietary α-tocopheryl acetate and ascorbic acid on rabbit semen. Theriogenology 54, 523–533.CrossRefGoogle ScholarPubMed
Castellini, C, Lattaioli, P, Dal Bosco, A, Minelli, A, Mugnai, C 2003. Oxidative status and semen characteristics of rabbit buck as affected by dietary vitamin E, C and n-3 fatty acids. Reproduction Nutrition Development 43, 91–103.CrossRefGoogle Scholar
Cerolini, S, Kelso, KA, Noble, RC, Speake, BK, Pizzi, F, Cavalchini, LG 1997. Relationship between spermatozoan lipid composition and fertility during aging of chickens. Biology of Reproduction 57, 976–980.CrossRefGoogle ScholarPubMed
Chen, JK, Wang, DW, Falck, JR, Capdevila, J, Harris, RC 1999. Transfection of an active cytochrome P450 arachidonic acid epoxygenase indicates that 14,15-epoxyeicosatrienoic acid functions as an intracellular second messenger in response to epidermal growth factor. Journal of Biological Chemistry 274, 4764–4769.CrossRefGoogle ScholarPubMed
Chiu, DTY, Stults, FH, Tappel, AL 1976. Purification and properties of rat lung soluble glutathione peroxidase. Biochimica et Biophysica Acta 445, 558–566.CrossRefGoogle ScholarPubMed
Correa, JR, Zavos, PM 1996. Preparation and recovery of frozen–thawed bovine spermatozoa via various sperm selection techniques employed in assisted reproductive technologies. Theriogenology 46, 1225–1232.CrossRefGoogle ScholarPubMed
Das, KS, Vasudevan, DM 2006. Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity. Indian Journal of Clinical Biochemistry 21, 62–69.CrossRefGoogle ScholarPubMed
Das, KS, Gupta, G, Rao, DN, Vasudevan, DM 2007. Effect of lecithin with vitamin-B complex and tocopheryl acetate on long term effect of ethanol induced immunomodulatory activities. Indian Journal of Experimental Biology 45, 683–688.Google ScholarPubMed
El-Deek, AA, Attia, YA, Al-Harthi, MA 2010. Including whole inedible date in grower-finisher broiler diets and the impact on productive performance, nutrient digestibility and meat quality. Animal 4, 1647–1652.CrossRefGoogle ScholarPubMed
Etches, RJ 1996. The male. In Reproduction in poultry, pp. 209–233. CAB International, Wallingford, UK.Google Scholar
Fellner, SJ, Sauer, FD, Kramer, JGK 1995. Steady-state rates of linoleic acid biohydrogenation by ruminal bacteria in continuous culture. Journal of Dairy Science 78, 1815–1823.CrossRefGoogle ScholarPubMed
Fiume, Z 2001. Final report on the safety assessment of lecithin and hydrogenated lecithin. International Journal of Toxicology 20 (suppl. 1), 21–45.Google ScholarPubMed
Gromadzka, OJ, Przepiórka, M, Romanowicz, K 2002. Influence of dietary fatty acids composition, level of dietary fat and feeding period on some parameters of androgen metabolism in male rats. Reproductive Biology 2, 277–293.Google Scholar
Gurr, MI, Harwood, JL, Frayn, KN 2002. Lipid biochemistry: an introduction, 5th edition. Blackwell Science Ltd, Oxford, UK.CrossRefGoogle Scholar
Habig, WH, Pabst, MJ, Jakoby, WB 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249, 7130–7139.CrossRefGoogle ScholarPubMed
Hansen, HS 1989. Linoleic acid and epidermal water barrier. In Dietary omega-3 and omega-6 fatty acids, biological effects and nutritional essentiality (ed. C Galli and AP Simopoulos), pp. 333–342. Plenum Press, New York, USA.Google Scholar
Holland, JL, Kronfeld, DS, Rich, GA, Kline, KA 1998. Acceptance of fat and lecithin containing diets by horses. Applied Animal Behaviour Science 56, 91–96.CrossRefGoogle Scholar
Holman, RT 1998. The slow discovery of the importance of omega 3 essential fatty acids in human health. Journal of Nutrition 128 (2), 427S–433S.CrossRefGoogle ScholarPubMed
IRRG (International Rabbit Reproduction Group) 2005. Recommendations and guidelines for applied reproduction trials with rabbit does. World Rabbit Science 13, 147–164.Google Scholar
Jenkins, TC, Fotouhi, N 1990. Effects of lecithin and corn oil on site of digestion, ruminal fermentation and microbial protein synthesis in sheep. Journal Animal Science 68, 460–466.CrossRefGoogle ScholarPubMed
Judde, A, Villeneuve, P, Rossignol-Castera, A, Le Guillou, A 2003. Antioxidant effect of soy lecithins on vegetable oil stability and their synergism with tocopherols. Journal of the American Oil Chemistry Society 80, 1209–1215.CrossRefGoogle Scholar
Kelso, KA, Cerolini, S, Noble, RC, Sparks, NHC, Speake, BK 1996. Lipid and antioxidant changes in semen of broiler fowl from 25 to 60 weeks of age. Journal of Reproduction and Fertility 106, 201–206.CrossRefGoogle ScholarPubMed
Korach, KS 1997. Steroid hormones in endocrinology. In Basic and clinical principles (ed. PM Conn and S Melmed), pp. 157–239. Human Press Inc., Totowa, NY, USA.Google Scholar
Langlais, J, Roberts, D 1985. A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa. Gamete Research 12, 183–224.CrossRefGoogle Scholar
Lechowski, R, Bielecki, W, Sawosz, E, Krawiec, M, Klucinński, W 1999. The effect of lecithin supplementation on the biochemical profile and morphological changes in the liver of rats fed different animal fats. Veterinary Research Communications 23, 1–14.CrossRefGoogle ScholarPubMed
Liu, KKM, Barrows, FT, Hardy, RW, Dong, FM 2004. Body composition, growth performance, and product quality of rainbow trout (Oncorhynchus mykiss) fed diets containing poultry fat, soybean/corn lecithin, or menhaden oil. Aquaculture 238, 309–328.CrossRefGoogle Scholar
Lough, DS, Solomon, MB, Rumsey, TS, Elsasser, TH, Slyter, LL, Kahl, S, Lynch, GP 1991. Effects of dietary canola seed and soy lecithin in high-forage diets on performance, serum lipids, and carcass characteristics of growing ram lambs. Journal Animal Science 69, 3292–3298.CrossRefGoogle ScholarPubMed
Maldjian, A, Penny, P, Noble, R 2003. Docosahexaenoic acid-rich marine oils and improved reproductive efficiency in pigs. In Male fertility and lipid metabolism (ed. AB Christophe and SR De Vriese), pp. 60–72. AOAC, Gaithersburg, MD, USA.Google Scholar
Mann, T, Lutwak-Mann, C 1981. Male reproductive function and semen. Springer-Verlag, Berlin, Germany.CrossRefGoogle Scholar
Misra, HP, Fridovich, I 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry 247, 3170–3175.CrossRefGoogle Scholar
Mohn, CE, Fernandez-Solari, J, De Laurentiis, A, Prestifilippo, JP, de la Cal, C, Funk, R, Bornstein, SR, McCann, SM, Rettori, V 2005. The rapid release of corticosterone from the adrenal induced by ACTH is mediated by nitric oxide acting by prostaglandin E2. Proceedings of the National Academy of Science USA 102, 6213–6218.CrossRefGoogle ScholarPubMed
Mourvaki, E, Cardinali, R, Dal Bosco, A, Corazzi, L, Castellini, C 2010. Effects of flaxseed dietary supplementation on sperm quality and on lipid composition of sperm subfractions and prostatic granules in rabbit. Theriogenology 73, 629–637.CrossRefGoogle ScholarPubMed
National Research Council (NRC) 1977. Nutrient requirements of rabbits, 2nd revised edition. National Academy of Science, Washington, DC, USA.Google Scholar
National Research Council (NRC) 1994. Nutrient requirements of poultry. 9th revised edn. National Academy Press, Washington, DC, USA.Google Scholar
Newberry, RD, Stenson, WF, Lorenz, RG 1999. Cyclooxygenase-2-dependent arachidonic acid metabolites are essential modulators of the intestinal immune response to dietary antigen. Nature Medicine 5, 900–906.CrossRefGoogle ScholarPubMed
Olafsdottir, K, Reed, DJ 1988. Retention of oxidized glutathione by isolated rat liver mitochondria during hydroperoxide treatment. Biochimica et Biophysica Acta 964, 377–382.CrossRefGoogle ScholarPubMed
Rocha, AA, Cunha, ICN, Ederli, BB, Albernaz, AP, Quirino, CR 2009. Effect of daily food supplementation with essential fatty acids on canine semen quality. Reproduction in Domestic Animals 44 (suppl. 2), 313–315.CrossRefGoogle ScholarPubMed
Scholfield, CR 1981. Composition of soybean lecithin. The Journal of the American Oil Chemists’ Society 58, 889–892.CrossRefGoogle Scholar
Simpson, ER, Mahendroo, MS, Means, GD, Kilgore, MW, Hinshelwood, MM, Graham-Lorence, S, Bilal, A, Ito, Y, Fisher, CR, Michael, MD, Mendelson, CR, Bulun, SE 1994. Aromatase Cytochrome P450, the enzyme responsible for estrogen biosynthesis. Endocrine Reviews 15, 342–355.Google ScholarPubMed
Smith, JT, Mayer, DT 1955. Evaluation of sperm concentration by the hemocytometer method. Comparison of four counting fluids. Fertility and Sterility 6, 271–275.CrossRefGoogle Scholar
Soares, M, Lopez-Bote, CJ 2002. Effects of dietary lecithin and fat unsaturation on nutrient utilisation in weaned piglets. Animal Feed Science and Technology 95, 169–177.CrossRefGoogle Scholar
Statistical Analysis System (SAS) 1996. SAS user's guide: statistics, version, 6th edition. SAS Institute Inc., Cary, NC, USA.Google Scholar
Tappel, AL, Zalkin, H 1959. Inhibition of lipid peroxidation in mitochondria by vitamin E. Archives of Biochemistry and Biophysics 80, 333–336.CrossRefGoogle Scholar
Ulkowski, M, Musialik, M, Litwinienko, G 2005. Use of differential scanning calorimetry to study lipid oxidation. 1. Oxidative stability of lecithin and linolenic acid. Journal of Agricultural and Food Chemistry 53, 9073–9077.CrossRefGoogle ScholarPubMed
Walker, WH 2009. Molecular mechanisms of testosterone action in spermatogenesis. Steroids 74, 602–607.CrossRefGoogle ScholarPubMed
Wang, G, Wang, T 2008. Oxidative stability of egg and soy lecithin as affected by transition metal ions and pH in emulsion. Journal of Agricultural and Food Chemistry 56, 11424–11431.CrossRefGoogle ScholarPubMed