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Stable isotopes to study sulfur amino acid utilization in broilers

Published online by Cambridge University Press:  10 June 2020

R. M. Suzuki Open the ORCID record for R. M. Suzuki [Opens in a new window] ,
L. G. Pacheco Open the ORCID record for L. G. Pacheco [Opens in a new window] ,
J. C. P. Dorigam Open the ORCID record for J. C. P. Dorigam [Opens in a new window] ,
J. C. Denadai Open the ORCID record for J. C. Denadai [Opens in a new window] ,
G. S. Viana Open the ORCID record for G. S. Viana [Opens in a new window] ,
H. R. Varella ,
C. C. N. Nascimento Open the ORCID record for C. C. N. Nascimento [Opens in a new window] ,
J. Van Milgen Open the ORCID record for J. Van Milgen [Opens in a new window]  and
N. K. Sakomura Open the ORCID record for N. K. Sakomura [Opens in a new window]
R. M. Suzuki
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
L. G. Pacheco
Affiliation:
Department of Veterinary Clinic and Surgery, São Paulo State University, Jaboticabal, 148830-900, Brazil
J. C. P. Dorigam
Affiliation:
Evonik Nutrition & Care GmbH, Hanau, 63457, Germany
J. C. Denadai
Affiliation:
Stable Isotope Center, São Paulo State University, Botucatu, 18618-689, Brazil
G. S. Viana
Affiliation:
Natural Resources Institute Finland, Luke, Jokioinen, 31600, Finland
H. R. Varella
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
C. C. N. Nascimento
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
J. Van Milgen
Affiliation:
UMR1348 PEGASE, INRA, Agrocampus Ouest, Saint-Gilles, 35590, France
N. K. Sakomura*
Affiliation:
Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil
*
†E-mail: nilva.sakomura@unesp.br
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Abstract

Nutritionists have been discussing whether the dietary supplementation of cyst(e)ine is required as a part of the dietary methionine (Met) in the total sulfur amino acid (TSAA) requirement to achieve optimum performance in broilers. Part of Met is converted to cysteine (Cys) to meet the Cys requirement, especially for feather growth. The TSAA requirement has been determined by using graded levels of free Met in the diet, without supplementation of free cyst(e)ine. It has also been argued that the Met to Cys ratio (Met : Cys) changes with age and even with different Met sources. The objective of this study was to evaluate the two sources of Met, while determining the proportion of Met and Cys in total dietary TSAA that optimize the performance of broilers. A performance assay was carried out in a factorial arrangement (5 × 2) using 1080 broilers from 42 to 56 days of age fed diets having different dietary proportions of Met and Cys (44 : 56, 46 : 54, 48 : 52, 50 : 50 or 52 : 48) while maintaining the same dietary TSAA in the diets. Two synthetic Met sources (dl-Met or l-Met) were used for each of the diets with different dietary Met : Cys ratios. Twenty-one broilers of the same age were fed the diets 44 : 56, 48 : 52 and 52 : 48 by supplementing the diet with L-(15N) Met or L-(15N2) Cystine to study the metabolism of TSAA. No differences were observed between Met sources for feed intake, BW gain and feed conversion ratio (FCR; P > 0.05); however, FCR was numerically improved at 50 : 50 Met : Cys. Regarding TSAA utilization, the conversion of Met to Cys increased with increase in Met : Cys ratios, but the concentration of Met intermediates decreased. Broiler chickens responded to different dietary proportions of sulfur amino acids by altering their sulfur amino acid metabolism, and diets containing 50 : 50 Met : Cys is recommended for broilers of age 42 to 56 days.

Keywords

broilercysteinelabeled amino acidmethioninetranssulfuration
Type
Research Article
Information
animal , Volume 14 , Issue S2: 9th Workshop on Modelling Nutrient Digestion and Utilization in Farm Animals (MODNUT) , August 2020 , pp. s286 - s293
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

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Footnotes

a

Present address: Department of Animal Science, São Paulo State University, Poultry Science Laboratory, Lavinesp, Via de Acesso Professor Paulo Donato Castelane Castellane s/n 14883-900, Jaboticabal, Brazil

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