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Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows

Published online by Cambridge University Press:  02 May 2016

E. Vargas-Bello-Pérez ,
N. Cancino-Padilla  and
J. Romero
E. Vargas-Bello-Pérez*
Affiliation:
Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Casilla 306, C.P. 6904411, Chile
N. Cancino-Padilla
Affiliation:
Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Casilla 306, C.P. 6904411, Chile
J. Romero
Affiliation:
Laboratorio de Biotecnología, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, C.P. 7810000, Chile
*
†E-mail: evargasb@uc.cl
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Abstract

Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.

Keywords

rumenyeast identificationdairy cowinternal transcribed spacer
Type
Research Article
Information
animal , Volume 10 , Issue 12 , December 2016 , pp. 1949 - 1954
Copyright
© The Animal Consortium 2016 

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