Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-23T14:06:26.160Z Has data issue: false hasContentIssue false
  • English
  • Français

Age-related oxidative stress and antioxidant capacity in heat-stressed broilers

Published online by Cambridge University Press:  23 February 2017

A. P. Del Vesco ,
A. S. Khatlab ,
E. S. R. Goes ,
K. S. Utsunomiya ,
J. S. Vieira ,
A. R. Oliveira Neto  and
E. Gasparino
A. P. Del Vesco
Affiliation:
Department of Animal Science, Universidade Estadual de Maringá, Colombo Avenue, 5790Maringá, Paraná, Brazil
A. S. Khatlab
Affiliation:
Department of Animal Science, Universidade Estadual de Maringá, Colombo Avenue, 5790Maringá, Paraná, Brazil
E. S. R. Goes
Affiliation:
Department of Animal Science, Universidade Estadual de Maringá, Colombo Avenue, 5790Maringá, Paraná, Brazil
K. S. Utsunomiya
Affiliation:
Department of Animal Science, Universidade Estadual de Maringá, Colombo Avenue, 5790Maringá, Paraná, Brazil
J. S. Vieira
Affiliation:
Department of Animal Science, Universidade Federal de Sergipe, Marechal Rondon Avenue, S/N, São Cristóvão, Sergipe, Brazil
A. R. Oliveira Neto
Affiliation:
Evonik Degussa of Brazil, Alameda Campinas, 579São Paulo, São Paulo, Brazil
E. Gasparino*
Affiliation:
Department of Animal Science, Universidade Estadual de Maringá, Colombo Avenue, 5790Maringá, Paraná, Brazil
*
†E-mail: egasparino@uem.br
Get access

Abstract

We aimed to evaluate the effects of acute heat stress (HS) and age on the redox state in broilers aged 21 and 42 days. We evaluated the expression of genes related to antioxidant capacity, the production of hydrogen peroxide (H2O2), and the activity of antioxidant enzymes in the liver, as well as oxidative stress markers in the liver and plasma. The experiment had a completely randomized factorial design with two thermal environments (thermoneutral and HS, 38°C for 24 h) and two ages (21 and 42 days). Twenty-one-day-old animals exposed to HS showed the highest thioredoxin reductase 1 (TrxR1) (P<0.0001) and glutathione synthetase (GSS) (P<0.0001) gene expression levels. Age influenced the expression of the thioredoxin (Trx) (P=0.0090), superoxide dismutase (SOD) (P=0.0194), glutathione reductase (GSR) (P<0.0001) and glutathione peroxidase 7 (GPx7) (P<0.0001) genes; we observed greater expression in birds at 21 days than at 42 days. Forty-two-day-old HS birds showed the highest H2O2 production (222.31 pmol dichlorofluorescein produced/min×mg mitochondrial protein). We also verified the effects of age and environment on the liver content of Glutathione (GSH) (P<0.0001 and P=0.0039, respectively) and catalase (CAT) enzyme activity (P=0.0007 and P=0.0004, respectively). Higher GSH content and lower CAT activity were observed in animals from the thermoneutral environment compared with the HS environment and in animals at 21 days compared with 42 days. Broilers at 42 days of age had higher plasma creatinine content (0.05 v. 0.01 mg/dl) and higher aspartate aminotransferase activity (546.50 v. 230.67 U/l) than chickens at 21 days of age. Our results suggest that under HS conditions, in which there is higher H2O2 production, 21-day-old broilers have greater antioxidant capacity than 42-day-old animals.

Keywords

ageantioxidant capacityheat stressoxidative stressROS
Type
Research Article
Information
animal , Volume 11 , Issue 10 , October 2017 , pp. 1783 - 1790
Copyright
© The Animal Consortium 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

a

Present address: Department of Animal Science, Universidade Federal de Sergipe, Marechal Rondon Avenue, S/N, São Cristóvão, Sergipe, Brazil.

References

Aebi, H 1974. Catalase. In Methods of enzymatic analysis (ed. HU Bergmeyer), pp. 673–690. Academic Press, New York, NY, USA.CrossRefGoogle Scholar
Ahmed, RJ 2005. Heat stress induced histopathology and pathophysiology of the central nervous system. International Journal of Developmental Neuroscience 23, 549–557.Google Scholar
Aluwong, T, Kawu, M, Raji, M, Dzenda, T, Govwang, F, Sinkalu, V and Ayo, J 2013. Effect of yeast probiotic on growth, antioxidant enzyme activities and malondialdehyde concentration of broiler chickens. Antioxidants 2, 326–339.CrossRefGoogle ScholarPubMed
Berson, A, De Beco, V, Lettéron, P, Robin, MA, Moreau, C, El Kahwaji, J, Verthier, N, Feldmann, G, Fromenty, B and Pessayre, D 1998. Steatohepatitis-inducing drugs cause mitochondrial dysfunction and lipid peroxidation in rat hepatocytes. Gastroenterology 114, 764–774.CrossRefGoogle ScholarPubMed
Bracht, A, Ishii-Iwamoto, EL and Salgueiro-Pagadigorria, CL 2003. Técnica de centrifugação e fracionamento celular. In Métodos de Laboratório em Bioquímica (ed. A, Bracht and EL, Ishii-Iwamoto), pp. 77–101. Manole, São Paulo, Brazil.Google Scholar
Cui, H, Kong, Y and Zhang, H 2012. Oxidative stress, mitochondrial dysfunction, and aging. Journal of Signal Transduction 2012, 1–13.Google Scholar
Das, S, Palai, TK, Mishra, SR, Das, D and Jena, B 2011. Nutrition in relation to diseases and heat stress in poultry. Veterinary World 4, 429–432.Google Scholar
Fernandes, WR and Larsson, MHMA 2000. Alterações nas concentrações séricas de glicose, sódio, ureia e creatinina, em equinos submetidos a provas de enduro de 30 Km com velocidade controlada. Ciência Rural 30, 393–398.CrossRefGoogle Scholar
Ghezzi, P 2013. Protein glutathionylation in health and disease. Biochimica et Biophysica Acta 1830, 3165–3172.CrossRefGoogle ScholarPubMed
Gomes-Marcondes, MC and Tisdale, MJ 2002. Induction of protein catabolism and the ubiquitin-proteasome pathway by mild oxidative stress. Cancer Letters 180, 69–74.Google Scholar
Gutteridge, JMC and Mitchell, J 1999. Redox imbalance in the critically ill. British Medical Bulletin 55, 49–75.Google Scholar
Hissin, PJ and Hilf, R 1976. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical Biochemistry 74, 214–226.Google Scholar
Huber, PC, Almeida, WP and Fátima, A 2008. Glutationa e enzimas relacionadas: Papel biológico e importância em processos patológicos. Quimica Nova 31, 1170–1179.Google Scholar
Khan, HA, Alhomida, AS, Sobki, SH, Habib, SS, Aseri, ZA, Khan, AA and Moghairi, AA 2013. Serum markers of tissue damage and oxidative stress in patients with acute myocardial infarction. Biomedical Research 24, 15–20.Google Scholar
Koháryová, M and Kollárová, M 2008. Oxidative stress and thioredoxin system. General Physiology and Biophysics 27, 71–84.Google Scholar
Lee, J, Giordano, S and Zhang, J 2012. Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling. The Biochemical Journal 441, 523–540.Google Scholar
Lin, H, Decuypere, E and Buyse, J 2006. Acute heat stress induces oxidative stress in broiler chickens. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 144, 11–17.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using realtimequantitative PCR and the 2−∆∆CT method. Methods 25, 402–408.Google Scholar
Lou, MF 2003. Redox regulation in the lens. Progress in Retinal and Eye Research 22, 657–682.Google Scholar
Mahmoud, KZ and Edens, FW 2003. Influence of selenium sources on age-related and mild heat stress related changes of blood and liver glutathione redox cycle in broiler chickens (Gallus domesticus). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology 136, 921–934.Google Scholar
Marklund, S and Marklund, G 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47, 469–474.Google Scholar
Morand, C, Rios, L, Moundras, C, Besson, C, Remesy, C and Demigne, C 1997. Influence of methionine availability on glutathione synthesis and delivery by the liver. The Journal of Nutritional Biochemistry 8, 246–255.Google Scholar
Mujahid, A, Sato, K, Akiba, Y and Toyomizu, M 2006. Acute heat stress stimulates mitochondrial superoxide production in broiler skeletal muscle, possibly via downregulation of uncoupling protein content. Poultry Science 85, 1259–1265.Google Scholar
Nishikawa, M, Hashida, M and Takakura, Y 2009. Catalase delivery for inhibiting ROS-mediated tissue injury and tumor metastasis. Advanced Drug Delivery Reviews 61, 19–326.CrossRefGoogle ScholarPubMed
Ohkawa, H, Ohishi, N and Yagi, K 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95, 351–358.Google Scholar
Oktyabrsky, ON and Smirnova, GV 2007. Redox regulation of cellular functions. Biochemistry 72, 132–145.Google Scholar
Pamok, S, Aengwanich, W and Komutrin, T 2009. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat-stressed broilers. Journal of Thermal Biology 34, 353–357.Google Scholar
Rostagno, HS, Albino, LFT, Donzele, JL, Gomes, PC, Oliveira, RF, Lopes, DC, Ferreira, AS and Barreto, SLT 2011. Brazilian tables for birds and pigs: composition of foods and nutritional requirements, 3rd edition. UFV, Viçosa, Brazil.Google Scholar
Sandercock, DA, Hunter, RR, Nute, GR, Mitchell, MA and Hocking, PM 2001. Acute heat stress-induced alterations in blood acid-base status and skeletal muscle membrane integrity in broiler chickens at two ages: implications for meat quality. Poultry Science 80, 418–425.Google Scholar
Song, Z, Liu, L, Sheikhahmadi, A, Jiao, H and Lin, H 2012. Effect of heat exposure on gene expression of feed intake regulatory peptides in laying hens. Journal of Biomedicine and Biotechnology 2012, 1–8.Google ScholarPubMed
Tan, SX, Greetham, D, Raeth, S, Grant, CM, Dawes, IW and Perrone, GG 2010. The thioredoxin-thioredoxin reductase system can function in vivo as an alternative system to reduce oxidized glutathione in Saccharomyces cerevisiae . The Journal of Biological Chemistry 285, 6118–6126.Google Scholar
Tengan, CH, Gabbai, AA and Moraes, CT 1998. Deleções do DNA mitocondrial no envelhecimento: efeito da disfunção na fosforilação oxidativa. Revista de Psiquiatria Cliníca 25, 126–131.Google Scholar
Willemsen, H, Swennen, Q, Everaert, N, Geraert, PA, Mercier, Y, Stinckens, A, Decuypere, E and Buyse, J 2011. Effects of dietary supplementation of methionine and its hydroxy analog DL-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasm hormone levels, and the redox status of broiler chickens expose to high temperatures. Poultry Science 90, 2311–2320.Google Scholar
Wu, G 2013. Amino acids: biochemistry and nutrition. Taylor & Francis Group, New York, NY, USA.Google Scholar
Yang, GY, Tan, QF, Fu, JH, Feng, J and Zhang, MZ 2010. Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration, ROS production and lipid peroxidation in broiler chickens. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 151, 204–208.Google Scholar