Hostname: page-component-7479d7b7d-fwgfc Total loading time: 0 Render date: 2024-07-08T14:30:44.289Z Has data issue: false hasContentIssue false
  • English
  • Français

Nematodes from the Victoria Land coast, Antarctica and comparisons with cultured Panagrolaimus davidi

Published online by Cambridge University Press:  13 May 2013

Mélianie R. Raymond ,
David A. Wharton  and
Craig J. Marshall
Mélianie R. Raymond
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
David A. Wharton*
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
Craig J. Marshall
Affiliation:
Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand
*
*Corresponding author: david.wharton@otago.ac.nz
Get access Rights & Permissions [Opens in a new window]

Abstract

Morphological and genetic information was used to determine the species identity and relationships of four species of nematodes from the Victoria Land coast, Antarctica; and between laboratory and field populations of Panagrolaimus davidi Timm. The 18S and D3 expansion segments of 28S rRNA were successfully amplified from the four species: Scottnema lindsayae Timm, Plectus murrayi Yeates, Eudorylaimus spp. and Panagrolaimus davidi. The four species from field populations mapped to three different nematode clades, indicating that if they are survivors in, rather than colonizers of, Antarctica they originate from several Gondwanan ancestors. The laboratory culture of P. davidi is shown to be a different species to that of the field samples of P. davidi, which were genetically identical to each other. There are several possible explanations for this observation, which require further investigation.

Keywords

Eudorylaimus sp.field and laboratory strainPlectus murrayiScottnema lindsayae
Type
Biological Sciences
Information
Antarctic Science , Volume 26 , Issue 1 , February 2014 , pp. 15 - 22
Copyright
Copyright © Antarctic Science Ltd 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abolafia, J. Pena-Santiago, R. 2005. Nematodes of the order Rhabditida from Andalucia Oriental, Spain. The family Panagrolaimidae, with a compendium of species of Panagrolaimus and a key to their identification. Journal of Nematode Morphology and Systematics, 8, 133–159.Google Scholar
Adams, B.J., Wall, D.H., Gozel, U., Dillman, A.R., Chaston, J.M. Hogg, I.D. 2007. The southernmost worm, Scottnema lindsayae (Nematoda): diversity, dispersal and ecological stability. Polar Biology, 30, 809–815.CrossRefGoogle Scholar
Adams, B.J., Bardgett, R.D., Ayres, E., Wall, D.H., Aislabie, J., Bamforth, S., Bargagli, R., Cary, C., Cavacini, P., Connell, L., Convey, P., Fell, J.W., Frati, F., Hogg, I.D., Newsham, K.K., O'Donnell, A., Russell, N., Seppelt, R.D. Stevens, M.I. 2006. Diversity and distribution of Victoria Land biota. Soil Biology & Biochemistry, 38, 3003–3018.Google Scholar
Andrassy, I. 1998. Nematodes in the sixth continent. Journal of Nematode Morphology and Systematics, 1, 107–186.Google Scholar
Andrassy, I. 2008a. Eudorylaimus species (Nematoda: Dorylaimida) of continental Antarctica. Journal of Nematode Morphology and Systematics, 11, 49–66.Google Scholar
Andrassy, I. 2008b. On the male of the Antarctic nematode species, Plectus murrayi Yeates, 1970. Journal of Nematode Morphology and Systematics, 11, 87–89.Google Scholar
Barrett, J.E., Virginia, R.A., Wall, D.H., Cary, S.C., Adams, B.J., Hacker, A.L. Aislabie, J.M. 2006. Co-variation in soil biodiversity and biogeochemistry in northern and southern Victoria Land, Antarctica. Antarctic Science, 18, 535–548.CrossRefGoogle Scholar
Blaxter, M. 1998. Caenorhabditis elegans is a nematode. Science, 282, 2041–2046.CrossRefGoogle ScholarPubMed
Blaxter, M., Mann, J., Chapman, T., Thomas, F., Whitton, C., Floyd, R. Abebe, E. 2005. Defining operational taxonomic units using DNA barcode data. Philosophical Transactions of the Royal Society, B360, 1935–1943.CrossRefGoogle Scholar
Blaxter, M.L., De Ley, P., Garey, J.R., Liu, L.X., Scheldeman, P., Vierstraete, A., Vanfleteren, J.R., Mackey, L.Y., Dorris, M., Frisse, L.M., Vida, J.T. Thomas, W.K. 1998. A molecular evolutionary framework for the phylum Nematoda. Nature, 392, 71–75.CrossRefGoogle ScholarPubMed
Boström, S. 1995. Populations of Plectus accuminatus Bastian, 1865 and Panagrolaimus magnivulvatus n. sp. (Nematoda) from nunataks in Dronning Maud Land, East Antarctica. Fundamental and Applied Nematology, 18, 25–34.Google Scholar
Boström, S., Holovachov, O. Nadler, S. 2011. Description of Scottnema lindsayae Timm, 1971 (Rhabditida: Cephalobidae) from Taylor Valley, Antarctica and its phylogenetic relationship. Polar Biology, 34, 1–12.CrossRefGoogle Scholar
Convey, P., Gibson, J.A.E., Hillenbrand, C.D., Hodgson, D.A., Pugh, P.J.A., Smellie, J.L. Stevens, M.I. 2008. Antarctic terrestrial life - challenging the history of the frozen continent? Biological Reviews, 83, 103–117.CrossRefGoogle ScholarPubMed
Courtright, E.M., Wall, D.H., Virginia, R.A., Frisse, L.M., Vida, J.T. Thomas, W.K. 2000. Nuclear and mitochondrial DNA sequence diversity in the Antarctic nematode Scottnema lindsayae . Journal of Nematology, 32, 143–153.Google ScholarPubMed
De Ley, P., Van de Velde, M.C., Mounport, D., Baujard, P. Coomans, A. 1995. Ultrastructure of the stoma in Cephalobidae, Panagrolaimidae, and Rhabditidae, with a proposal for a revised stroma terminology in Rhabditida (Nematoda). Nematologica, 41, 153–182.CrossRefGoogle Scholar
Denver, D.R., Morris, K., Lynch, M. Thomas, W.K. 2004. High mutation rate and predominance of insertions in the Caenorhabditis elegans nuclear genome. Nature, 430, 679–682.CrossRefGoogle ScholarPubMed
Eyualem, A. Blaxter, M. 2003. Comparison of biological, molecular, and morphological methods of species identification in a set of cultured Panagrolaimus isolates. Journal of Nematology, 35, 119–128.Google Scholar
Felsenstein, J. 1985. Confidence intervals on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.Google Scholar
Floyd, R., Abebe, E., Papert, A. Blaxter, M. 2002. Molecular barcodes for soil nematode identification. Molecular Ecology, 11, 839–850.CrossRefGoogle ScholarPubMed
Floyd, R.M., Rogers, A.D., Lambshead, P.J.D. Smith, C.R. 2005. Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5, 611–612.CrossRefGoogle Scholar
Hooper, D.J. 1986a. Handling, fixing, staining and mounting nematodes. In Southey, J.F. ed. Laboratory Methods for Work with Plant and Soil Nematodes. London: HMSO, 59–85.Google Scholar
Hooper, D.J. 1986b. Drawing and measuring nematodes. In Southey, J.F. ed. Laboratory methods for work with plant and soil nematodes. London: HMSO, 87–94.Google Scholar
Kito, K. Ohyama, Y. 2008. Rhabditid nematodes found from a rocky coast contaminated with treated wastewater of Casey Station in East Antarctica, with a description of a new species of Dolichorhabditis Andrassy, 1983 (Nematoda : Rhabditidae). Zootaxa, 1850, 43–52.CrossRefGoogle Scholar
Lewis, S.C., Dyal, L.A., Hilburn, C.F., Weitz, S., Liau, W.S., LaMunyon, C.W. Denver, D.R. 2009. Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the Antarctic species P. davidi . Bmc Evolutionary Biology, 9, 15.CrossRefGoogle ScholarPubMed
Maslen, N.R. Convey, P. 2006. Nematode diversity and distribution in the southern maritime Antarctic - clues to history? Soil Biology & Biochemistry, 38, 3141–3151.CrossRefGoogle Scholar
Nei, M. Kumar, S. 2000. Molecular evolution and phylogenetics. Oxford: Oxford University Press, 333 pp.CrossRefGoogle Scholar
Nunn, G.B., Theisen, B.F., Christensen, B. Arctander, P. 1996. Simplicity correlated size growth of the nuclear 28S ribosomal RNA D3 expansion segment in the crustacean order Isopoda. Journal of Molecular Evolution, 42, 211–223.CrossRefGoogle ScholarPubMed
Powers, T. 2004. Nematode molecular diagnostics: from bands to barcodes. Annual Review of Phytopathology, 42, 367–383.CrossRefGoogle ScholarPubMed
Raymond, M.R., Wharton, D.A. Marshall, C.J. 2013. Factors determining nematode distributions at Cape Hallett and Gondwana Station, Antarctica. Antarctic Science, 10.1017/S0954102012001162.CrossRefGoogle Scholar
Shannon, A.J., Browne, J.A., Boyd, J., Fitzpatrick, D.A. Burnell, A.M. 2005. The anhydrobiotic potential and molecular phylogenetics of species and strains of Panagrolaimus (Nematoda, Panagrolaimidae). Journal of Experimental Biology, 208, 2433–2445.CrossRefGoogle ScholarPubMed
Smith, T., Wharton, D.A. Marshall, C.J. 2008. Cold tolerance of an Antarctic nematode that survives intracellular freezing: comparisons with other nematode species. Journal of Comparative Physiology B, 178, 93–100.CrossRefGoogle ScholarPubMed
Tamura, K., Dudley, J., Nei, M. Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.CrossRefGoogle ScholarPubMed
Timm, R.W. 1971. Antarctic soil and freshwater nematodes from the McMurdo Sound region. Proceedings of the Helmintological Society of Washington, 38, 42–52.Google Scholar
Wharton, D.A. 1998. Comparison of the biology and freezing tolerance of Panagrolaimus davidi, an Antarctic nematode, from field samples and cultures. Nematologica, 44, 643–653.CrossRefGoogle Scholar
Wharton, D.A. Brown, I.M. 1989. A survey of terrestrial nematodes from the McMurdo Sound region, Antarctica. New Zealand Journal of Zoology, 16, 467–470.CrossRefGoogle Scholar
Wharton, D.A., Judge, K.F. Worland, M.R. 2000. Cold acclimation and cryoprotectants in a freeze-tolerant Antarctic nematode, Panagrolaimus davidi . Journal of Comparative Physiology B, 170, 321–327.Google Scholar
Yeates, G.W. 1969. Three new Rhabditida (Nematoda) from New Zealand dune sands. Nematologica, 15, 115–128.CrossRefGoogle Scholar
Yeates, G.W. 2010. Phylum Nematoda: roundworms, eelworms. In Gordon, D.P. ed. New Zealand Inventory of Biodiversity. Volume 2. Kingdom Animalia; Chaetognatha, Ecdysoa, Ichnofossils. Christchurch: Canterbury University Press, 480–493.Google Scholar
Yeates, G.W., Scott, M.B., Chown, S.L. Sinclair, B.J. 2009. Changes in soil nematode populations indicate an annual life cycle at Cape Hallett, Antarctica. Pedobiologia, 52, 375–386.CrossRefGoogle Scholar
Supplementary material: PDF

Raymond Supplementary Material

Figure

Download Raymond Supplementary Material(PDF)
PDF 422.1 KB