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Characterisation of genetic structure of Dicentrarchus labrax larvae in two nurseries of the Gulf of Lions (NW Mediterranean)

Published online by Cambridge University Press:  05 April 2008

Bruno Guinand ,
Emilie Dujardin ,
Vincent Dufour  and
Costas S. Tsigenopoulos
Bruno Guinand
Affiliation:
Université Montpellier II, Institut des Sciences de l'Evolution de Montpellier, Biologie intégrative, UMR CNRS 5554, place E. Bataillon, cc63, 34095 Montpellier Cedex 5, France École Pratique des Hautes Études, Station Méditerranéenne de l'Environnement Littoral, quai de la Daurade, 34200 Sète, France
Emilie Dujardin
Affiliation:
Université Montpellier II, Institut des Sciences de l'Evolution de Montpellier, Biologie intégrative, UMR CNRS 5554, place E. Bataillon, cc63, 34095 Montpellier Cedex 5, France
Vincent Dufour
Affiliation:
Université Montpellier II, Institut des Sciences de l'Evolution de Montpellier, Biologie intégrative, UMR CNRS 5554, place E. Bataillon, cc63, 34095 Montpellier Cedex 5, France École Pratique des Hautes Études, Station Méditerranéenne de l'Environnement Littoral, quai de la Daurade, 34200 Sète, France
Costas S. Tsigenopoulos
Affiliation:
Hellenic Centre for Marine Research, Institute of Marine Biology and Genetics, PO Box 2214, Gournes Pediados, 71500 Heraklion, Crete, Greece
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Abstract

Despite extensive knowledge of the genetic structure of sea bass (Dicentrarchus labrax) populations, no studies have investigated genetic structure within early life stages, or compared such structure between such stages and (sub)adults. Using nine newly developed microsatellite loci, we investigated patterns of genetic variation and relatedness among juveniles that settled in two NW Mediterranean nursery grounds in close geographical proximity. There was no evidence for differentiation among samples at settlement (θ = -0.0010), and no significant genetic relatedness. Nevertheless, significant departures from Hardy-Weinberg equilibrium were detected in each sample, which could not be attributed exclusively to the presence of null alleles and to Wahlund effect. These results are compared with and discussed in relation to the genetic structure of adults described in the same area 10 years ago.

Keywords

Population geneticsEuropean sea bassEarly life stagesAdultsNursery ground
Type
Research Article
Information
Aquatic Living Resources , Volume 21 , Issue 1 , January 2008 , pp. 81 - 87
Copyright
© EDP Sciences, IFREMER, IRD, 2008

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References

Allegrucci, G., Fortunato, C., Sbordoni, V., 1997, Genetic structure and allozyme variation of sea bass (Dicentrarchus labrax and D. punctatus) in the Mediterranean Sea. Mar. Biol. 128, 347–358. CrossRef
Allegrucci, G., Caccone, A., Cataudella, S., Powell, J.R., Sbordoni, V., 1995, Acclimation of the European sea bass to freshwater monitoring genetic changes by RAPD polymerase chain reaction to detect DNA polymorphisms. Mar. Biol. 121, 591–599. CrossRef
Bahri-Sfar, L., Lemaire, C., Ben Hassine, O.K., Bonhomme, F., 2000, Fragmentation of sea bass populations in the western and eastern Mediterranean as revealed by microsatellite polymorphism. Proc. R. Soc. Lond. B 267, 929–935. CrossRef
Bahri-Sfar, L., Lemaire, C., Chatain, B., Divanach, P., Ben Hassine, O.K., Bonhomme, F., 2005, Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax. Aquat. Living Resour. 18, 71–76. CrossRef
Behrmann-Godel, J., Gerlach, G., Eckmann, R., 2006, Kin and population recognition in sympatric Lake Constance perch (Perca fluviatilis L.): can assortative shoaling drive population divergence? Behav. Ecol. Sociobiol. 59, 451–468. CrossRef
Belkhir, K., Castric, V., Bonhomme, F., 2002, IDENTIX, a software to test for relatedness in a population using permutation methods. Mol. Ecol. Notes 2, 611–614. CrossRef
Benharrat, K., Pasteur, N., Siau, Y., Bouan, A., 1983, Polymorphisme biochimique de loups (Dicentrarchus labrax) originaires de quatre populations naturelles et d'un élevage. Rech. Biol. Aquacult. 1, 1–17.
Bierne, N., Tsitrone, A., David, P., 2000, An inbreeding model of associative overdominance during a population bottleneck. Genetics 155, 1981–1990.
Blouin, M.S., 2003, DNA-based methods for pedigree reconstruction and kinship analysis in natural populations. Trends Ecol. Evol. 18, 503–511. CrossRef
Burford, M.O., Larson, R.J., 2007, Genetic heterogeneity in a single year-class from a panmictic population of adult blue rockfish (Sebastes mystinus). Mar. Biol. 151, 451–465. CrossRef
Castric, V., Bernatchez, L., Belkhir, K., Bonhomme, F., 2002, Heterozygote deficiencies in small lacustrine populations of brook charr Salvelinus fontinalis Mitchill (Pisces, Salmonidae): a test of alternative hypotheses. Heredity 89, 27–35.
Chesser, R.K., 1991, Influence of gene flow and breeding tactics on gene diversity within populations. Genetics 129, 573–583.
Cockerham, C.C., 1969, Variance of gene frequencies. Evolution 23, 72–84. CrossRef
Cockerham, C.C., 1973, Analysis of gene frequencies. Genetics 74, 679–700.
Cushing, D.H., 1990, Plankton production and year class and year class strength in fish populations: an update of the match/mismatch hypothesis. Adv. Mar. Biol. 26, 249–293. CrossRef
Fritsch, M., Morizur, Y., Lambert, E., Bonhomme, F., Guinand, B., 2007, Assessment of sea bass (Dicentrarchus labrax, L.) stock delimitation in the Bay of Biscay and the English Channel based on mark-recapture and genetic data. Fish. Res. 83, 123–132. CrossRef
Garcia de León, F.J., Canonne, M., Quillet, E., Bonhomme, F., Chatain, B., 1998, The application of microsatellite markers to breeding programmes in the sea bass, Dicentrarchus labrax. Aquaculture 159, 303–316. CrossRef
Garcia de León, F.J., Chikhi, L., Bonhomme, F., 1997, Microsatellite polymorphism and population subdivision in natural populations of European sea bass Dicentrarchus labrax (Linnaeus, 1758). Mol. Ecol. 6, 51–62. CrossRef
Garcia de León, F.J., Dallas, J.F., Chatain, B., Canonne, M., Versini, J.J., Bonhomme, F., 1995, Development and use of microsatellite markers in sea bass, Dicentrarchus labrax (Linnaeus, 1758) (Perciformes: Serranidae). Mol. Mar. Biol. Biotech. 4, 62–68.
Guinand, B., Scribner, K.T., Page, K.S., Filcek, K., Main, L., Burnham-Curtis, M.K., 2006, Effects of coancestry on accuracy of individual assignments to population of origin: examples using Great Lakes lake trout (Salvelinus namaycush). Genetica 127, 329–340. CrossRef
Hedgecock D., 1994, Does variance in reproductive success limit effective population size of marine organisms? In: Beaumont A.R. (Ed.) Genetics and evolution of aquatic organisms, London, Chapman and Hall, pp. 122–134.
Huang, C.J., Lin, J.Y., Tsai, H.J., 1999, Two distinct c-ski cDNAs of fish, tilapia (Oreochromis aurea). Mol. Reprod. Dev. 54, 223–231. 3.0.CO;2-G>CrossRef
Johnson, M.S., Black, R., 1982, Chaotic genetic patchiness in an inter-tidal limpet, Siphonaria sp. Mar. Biol. 70, 157–164. CrossRef
Lee, B.Y., Lee, W.J., Streelman, J.T., Carleton, K.L., Howe, A.E., Hulata, G., Slettan, A., Stern, J.E., Terai, Y., Kocher, T.D., 2005, A second-generation genetic linkage map of tilapia (Oreochromis spp.). Genetics 170, 237–244. CrossRef
Leggett, W.C., Deblois, E., 1994, Recruitment in marine fishes: is it regulated by starvation and predation in the egg and larval stages? Neth. J. Sea Res. 32, 119–134. CrossRef
Lemaire, C., Allegrucci, G., Naciri, M., Bahri-Sfar, L., Kara, H., Bonhomme, F., 2000, Do discrepancies between microsatellite and allozyme variation reveal differential selection between sea and lagoon in the sea bass (Dicentrarchus labrax)? Mol. Ecol. 9, 457–467.
Moberg, P.E., Burton, R.S., 2000, Genetic heterogeneity among adult and recruit red sea urchins, Strongylocentrotus franciscanus. Mar. Biol. 136, 773–784. CrossRef
Petrenko, A., Leredde, I., Marsaleix, P., 2005, Circulation in a stratified and wind-forced Gulf of Lions, NW Mediterranean Sea: in situ and modeling data. Cont. Shelf Res. 25, 7–27. CrossRef
Queller, D., Goodnight, K., 1989, Estimating relatedness using genetic markers. Evolution 43, 258–275. CrossRef
Saillant, E., Bradfield, S.C., Gold, J.R., 2006, Genetic impacts of shrimp trawling on red snapper (Lutjanus campechanus) in the northern Gulf of Mexico. ICES J. Mar. Sci. 63, 705–713. CrossRef
Saillant, E., Mousseau, T.A., Gold, J.R., 2003, Genetic variation and relatedness of juvenile red snapper sampled from shrimp trawls in the northern Gulf of Mexico. Trans. Am. Fish. Soc. 132, 1229–1235. CrossRef
Selkoe, K.A., Gaines, S.D., Caselle, J.E., Warner, R.R., 2006, Current shifts and kin aggregation explain genetic patchiness in fish recruit. Ecology 87, 3082–3094. CrossRef
Tsigenopoulos, C.S., Hellemans, B., Chistiakov, D.A., Libertini, A., Kotoulas, G., Volckaert, F., 2003, Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.). Mol. Ecol. Notes 3, 352–354. CrossRef
Turner, T.F., Wares, J.P., Gold, J.R., 2002, Genetic effective size is three orders of magnitude smaller than adult census size in an abundant, estuarine-dependent marine fish (Sciaenops ocellatus). Genetics 162, 1239–1339.
van Oosterhout, C., Hutchinson, W.F., Willis, D.P.M., Shipley, P.F., 2004, Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes 4, 535–538. CrossRef
Weir, B.S., 1979, Inference about linkage disequilibrium. Biometrics 31, 235–254. CrossRef
Weir, B.S., Cockerham, C.C., 1984, Estimating F-statistics for the analysis of population structure. Evolution 38, 1358–1370.
Wright, S., 1951, The genetical structure of populations. Ann. Eugen. 15, 323–354. CrossRef