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Field study of metal concentrations and biomarker responsesin the neogastropod, Murex trunculus, from Bizerta Lagoon (Tunisia)

Published online by Cambridge University Press:  27 June 2008

Samia Gharbi-Bouraoui ,
Mauricette Gnassia-Barelli ,
Michèle Roméo ,
Mohamed Dellali  and
Patricia Aïssa
Samia Gharbi-Bouraoui
Affiliation:
Laboratory of Biomonitoring of the Environment, Coastal Ecology Unit, Faculty of Sciences of Bizerta, 7021 Zarzouna, Tunisia
Mauricette Gnassia-Barelli
Affiliation:
Ecology of Coastal Marine Ecosystems and Response to Stress, EA 4228 ECOMERS, Faculty of Sciences, University of Nice, Parc Valrose, BP 71, 06108 Nice Cedex 2, France
Michèle Roméo
Affiliation:
Ecology of Coastal Marine Ecosystems and Response to Stress, EA 4228 ECOMERS, Faculty of Sciences, University of Nice, Parc Valrose, BP 71, 06108 Nice Cedex 2, France
Mohamed Dellali
Affiliation:
Laboratory of Biomonitoring of the Environment, Coastal Ecology Unit, Faculty of Sciences of Bizerta, 7021 Zarzouna, Tunisia
Patricia Aïssa
Affiliation:
Laboratory of Biomonitoring of the Environment, Coastal Ecology Unit, Faculty of Sciences of Bizerta, 7021 Zarzouna, Tunisia
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Abstract

This study aims to evaluate the health status of Bizerta lagoon using a sentinel species, the Neogastropod Murex trunculus. Trace metal concentrations (Cd, Cu and Zn) in the foot and digestive gland, and biomarkers such as acetylcholinesterase (AChE), catalase (CAT) and glutathione transferase (GST) activities in the digestive gland, were determined in samples collected at four sites at different periods of the year. One site, in the North of the lagoon, is influenced by urban and industrial waste waters. Another, to the south, is located at the outfall of Ichkeul Lake. A third site is one that may be impacted by eutrophication processes, and the last, in the center of lagoon, is considered as less polluted. Temperature and salinity were simultaneously measured during the mollusc samplings. They varied greatly, from 13.5 °C and 30 psu in February to much higher values in summer-autumn: 30.7 °C and 37 psu. Metal concentrations were always higher in the digestive gland than in the foot. In the digestive gland, the lowest Cd and Cu concentrations were found in the center of the lagoon (mean ± standard deviation: 9.35 ± 0.78 µg Cd g−1 and 84.5 ± 15.3 µg Cu g−1) and no significant Zn variations were noted (682± 38 µg g−1) between sites. Metal concentrations in both types of tissue were higher in winter than in summer. AChE varied significantly among sites with high activities for individuals in the center of lagoon (15.4 ± 0.3 nmol min−1 mg-1 protein), whereas GST were low (12.2 ± 0.8 nmol min−1 mg−1 protein); CAT activities were low in this site 8.7 ± 0.5 µmol min-1 mg-1 protein. Season also had an influence on biomarkers, with low AChE in winter and rather low CAT and GST in summer. Principal component analysis showed that the center of lagoon can be considered as a reference site, whereas high chemical and biochemical responses were found in M. trunculus from other sites; particularly at the site which may be subject to eutrophication.

Keywords

Trace metalsCopperCadmiumZincBiomarkersMurex trunculusMediterranean Sea
Type
Research Article
Information
Aquatic Living Resources , Volume 21 , Issue 2 , April 2008 , pp. 213 - 220
Copyright
© EDP Sciences, IFREMER, IRD, 2008

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References

ANPE (Agence Nationale pour la Protection de l'Environnement), 1989, Diagnostic préliminaire pour l'étude de l'équilibre écologique du lac de Bizerte. Tunis, Tunisie: Rapp. ANPE; 72 p.
Bebianno, M.J., Langston, W.J., 1998, Cadmium and metallothionein turnover in different tissues of the gastropod Littorina littorea. Talanta 46, 301–313. CrossRef
Bebianno, M.J., Lopes, B., Guerra, L., Hoarau, P., Ferreira, A.M., 2007, Glutathione S-tranferases and cytochrome P450 activities in Mytilus galloprovincialis from the South coast of Portugal: effect of abiotic factors. Environ. Int. 33, 550–558. CrossRef
Bocquené G., Galgani F., Walker C.H., 1997, Les cholinestérases, biomarqueurs de toxicité. In: Lagadic, L., Caquet, T., Amiard, J.C., Ramade, F. (Eds.), Biomarqueurs en écotoxicologie. Aspects fondamentaux. Masson, Paris, pp. 209–239.
Bouquegneau, J.M., Canon, C., Martoja, M., 1988, Nouvelles données sur la teneur en cadmium de Murex trunculus (Gastéropode, Prosobranche) en milieu naturel non pollué. Océanis 4, 447–451.
Bouquegneau, J.M., Martoja, M., 1982, La teneur en cuivre et son degré de complexation chez quatre Gastéropodes marins. Données sur le cadmium et le zinc. Oceanol. Acta 5, 219–228.
Boyden, C.R., Phillips, D.J.H., 1981, Seasonal variation and inherent variability of trace elements in oysters and their implications for indicator studies. Mar. Ecol. Prog. Ser. 5, 29–40. CrossRef
Bradford, M., 1976, A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein–dye binding. Ann. Biochem. 72, 248–254. CrossRef
Burgeot, T., , Bocquené, G., Porte, C., Dimeet, J., Santella, R.M., Garcia de la Parra, L.M. Pfhol-Leszkowicz, A., Raoux, C. Galgani, F., 1996, Bioindicators of pollutant exposure in the northwestern Mediterranean Sea. Mar. Ecol. Prog. Ser. 131, 125–141. CrossRef
Cailleaud, K., Maillet, G., Budzinski, H., Souissi, S., Forget-Leray, J., 2007, Effects of salinity and temperature on the expression of enzymatic biomarkers in Eurytemora affinis (Calanoida, Copepoda). Comp. Biochem. Physiol. 147A, 841–849. CrossRef
Cajaraville, M.P., Bebianno, M.J., Blasco, J., Porte, C., Sarasquet, C., Viarengo, A., 2000, The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach. Sci. Total Environ. 247, 295–31. CrossRef
Canesi, L., Viarengo, A., Leonzio, C., Filippelli, M., Gallo, G., 1999, Heavy metals and glutathione metabolism in mussel tissues. Aquat. Toxicol. 46, 67–76. CrossRef
Catsiki, A.-V., Arnoux, A., 1987, Étude de la variabilité des teneurs en Hg, Cu, Zn et Pb de trois espèces de mollusques de l'étang de Berre (France). Mar. Environ. Res. 21, 175–187. CrossRef
Chouba L., Amara H., El Abed A., 2002, État de la contamination par les éléments traces des sédiments de la lagune de Bizerte. Bull. INSTM No. 7, Actes ATSMer, pp. 128–131.
Claiborne A., 1985, Catalase activity. In: Greenwald R.A. (Ed.) Handbook of Methods for Oxygen Radical Research, Boca Raton, CRC Press, pp. 283–284.
Cossu C., Doyotte A., Jacquin M.C., Vasseur P., 1997, Biomarqueurs du stress oxydant chez les animaux aquatiques. In: Lagadic, L., Caquet, T., Amiard, J.C., Ramade, F. (Eds.), Biomarqueurs en écotoxicologie. Aspects fondamentaux. Masson, Paris, pp. 149–163.
Dallinger, R., Carpenè, E., Dalla Via, G.J., Cortesi, P., 1989, Effects of cadmium on Murex trunculus from the Adriatic Sea: I. Accumulation of metal and binding to a metallothionein-like protein. Arch. Environ. Contam. Toxicol. 18, 554–561.
Damiens, G., His, E., Gnassia-Barelli, M., Quiniou, F., Roméo, M., 2004, Evaluation of biomarkers in oyster larvae in natural and polluted conditions. Comp. Biochem. Physiol. 138C, 121–128.
Dellali, M., Roméo, M., Aïssa, P., 2001, Suivi de l'activité catalase des moules et des palourdes originaires de la lagune de Bizerte. Oceanol. Acta 24, 263–271. CrossRef
Dellali M., Roméo M., Gnassia-Barelli M., Aïssa P., 2004, A Multivariate data analysis of the clam Ruditapes decussatus as sentinel organism of the Bizerta lagoon (Tunisia) Water, Air, Soil Pollut. 156, 131–144.
Ellman, G.L., Courtney, K.D., Andre, J.R.V., Featherstone, R.M., 1961, A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95. CrossRef
El Menif, N.T., Lahbib, Y., Le Pennec, M., Flower, R., Boumaïza, M., 2006, Intensity of the imposex phenomenon – impact on growth and fecundity in Hexaplex trunculus (Mollusca : Gastropoda) collected in Bizerta lagoon and channel (Tunisia). Cah. Biol. Mar. 47, 165–175.
Galgani, F., Bocquené, G., 1991, Semi-automated colorimetric and enzymatic assays for aquatic organisms using microplate readers. Water Res. 25, 147–150. CrossRef
Habig, W.H., Pabst, M.J., Jakobi, W.B., 1974, Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130–7139.
Harzallah A., 2003, Transport de polluants dans la lagune de Bizerte simulé par un modèle de la circulation de l'eau. Bull. Inst. Natn. Scien. Tech. Mer, Salammbô, 30, 121–132.
Julshamn K., Braekkan O.R., 1976, The relation between the concentration of some main elements and the stages of maturation of ovaries in cod (Gadus morrhua). Fisk. dir. Skr., Ernaering 1, 1–15.
Khessiba, A., Hoarau, P., Gnassia-Barelli, M., Aïssa, P., Roméo, M., 2001, Biochemical response of the mussel Mytilus galloprovincialis from Bizerta (Tunisia) to chemical pollutant exposure. Arch. Environ. Contam. Toxicol. 40, 222–229. CrossRef
Lahbib, Y., Boumaïza, M., El Menif, N.T., 2008, Imposex expression in Hexaplex trunculus from the North Tunis lake transplanted to Bizerta channel (Tunisia). Ecol. Ind. 8, 239–245. CrossRef
Lehtonen, K.K., Kankaanpää, H., Leiniö, S., Sipiä, V.O., Pflugmacher, S., Sandberg-Kilpi, E., 2003, Accumulation of nodularin-like compounds from the cyanobacterium Nodularia spumigena and changes in acetylcholinesterase activity in the clam Macoma balthica during short term laboratory exposure. Aquat. Toxicol. 64, 461–476. CrossRef
McCarthy J.F., Shugart L.R., 1990, Biomarkers of environmental contamination, Boca Raton, Lewis Publishers, pp. 3–14.
Pfeifer, S., Schiedek, D., Dippner, J.W., 2005, Effect of temperature and salinity on acetylcholinesterase activity, a common pollution biomarker, in Mytilus sp. from the south-western Baltic Sea. J. Exp. Mar. Biol. Ecol. 320, 93–103. CrossRef
Power A., Sheehan D., 1996, Seasonal variation in the antioxidant defence systems of gill and digestive gland of the blue mussel, Mytilus edulis. Comp. Biochem. Physiol. 114 C, 99–103.
Ramón, M., Amor, M.J., 2002, Reproductive cycle of Bolinus brandaris and penis and genital duct size variations in populations affected by imposex. J. Mar. Biol. Assoc. UK 82, 435–442. CrossRef
Roméo, M., 1987, Trace metals in fish roe from the Mauritania coast. Mar. Pollut. Bull. 18, 507–508. CrossRef
Roméo, M., Bennani, N., Gnassia-Barelli, M., Lafaurie, M., Girard, J.P., 2000, Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquat. Toxicol. 48, 185–194. CrossRef
Roméo, M., Gharbi-Bouraoui, S., Gnassia-Barelli, M., Dellali, M., Aïssa, P., 2006, Responses of Hexaplex (Murex) trunculus to selected pollutants. Sci. Total Environ. 359, 135–44. CrossRef
Scaps, P., Borot, O., 2000, Acetylcholinesterase activity of the polychaete Nereis diversicolor: effects of temperature and salinity. Comp. Biochem. Physiol. 125C, 377–383.
Stohs, S.J., Bagchi, D., Hassoun, E., Bagchi, M., 2000, Oxidative mechanisms in the toxicity of chromium and cadmium ions. J. Environ. Pathol. Toxicol. Oncol. 19, 201–213.
Trabelsi, S., Driss, M.R., 2005, Polycyclic aromatic hydrocarbons in superficial coastal sediments from Bizerte Lagoon, Tunisia. Mar. Pollut. Bull. 50, 344–348. CrossRef
Vasconcelos, P., Gaspar, M.B., Joaquim, S., Matias, D., Castro, M., 2004, Spawning of Hexaplex (Trunculariopsis) trunculus (Gastropoda: Muricidae) in the laboratory: description of spawning behaviour, egg masses, embryonic development, hatchling and juvenile growth rates. Invertebr. Reprod. Dev. 46, 125–138. CrossRef