Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-12T13:07:28.720Z Has data issue: false hasContentIssue false
  • English
  • Français

Absorption of lysine and methionine from the proximal colon of the piglet

Published online by Cambridge University Press:  09 March 2007

Alison J. Darragh ,
Peter D. Cranwell  and
Paul J. Moughan
Alison J. Darragh
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
Peter D. Cranwell
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
Paul J. Moughan
Affiliation:
Department of Animal Science, Massey University, Palmerston North, New Zealand
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study aimed to determine whether lysine and/or methionine are absorbed in nutritionally significant amounts from the proximal colon of milk-formula-fed piglets (15–32 d old; 2.0–7.4 kg liveweight). Piglets, surgically prepared with simple catheters which allowed infusion into the proximal colon, were randomly allocated to one of two milk-formula diets which were either 40 % deficient in lysine (L– diet) or 60% deficient in methionine and 40% deficient in cysteine (S – diet), yet balanced for all other amino acids. The piglets were individually bottle-fed the milk-formula diets seven times daily at 2 h intervals between 08.00 and 20.00 hours. Physiological saline (9 g NaCl/l) or an isotonic solution containing the deficient amino acid was infused via the catheter at each feeding. The experimental procedure followed a cross-over design. Total daily excretions of urinary urea and total N were determined. There were no significant differences (P > 0.05) in urinary N metabolite excretion for piglets infused with amino acids compared with those infused with saline. Lysine and methionine do not appear to be absorbed in nutritionally significant amounts from the proximal colon of the milk-fed piglet.

Keywords

PigletAmino acidsAbsorptionProximal colon
Type
absorption of lysine and methiionine from the colon
Information
British Journal of Nutrition , Volume 71 , Issue 5 , May 1994 , pp. 739 - 752
Copyright
Copyright © The Nutrition Society 1994

References

Agricultural Research Council (1981). The Nutrient Requirements of Pigs. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Association of Official Analytical Chemists (1980). Official Methods of Analysis, Vol. 14, Washington DC: Association of Official Analytical Chemists.Google Scholar
Batt, E. R. and Schachter, D. (1969). Developmental pattern of some intestinal transport mechanisms in newborn rats and mice. American Journal of Physiology 216, 1064–1068.Google Scholar
Binder, H. J. (1970). Amino acid absorption in the mammalian colon. Biochimica et Biophysica Acta 219, 503–506.Google Scholar
Brown, J. A. & Cline, T. R. (1972 a). Urea excretion in swine fed amino acid deficient diets. Journal of Animal Science 35, 211–212.Google Scholar
Brown, J. A. & Cline, T. R. (1972 b). Urinary urea and amino acid requirements of swine. Journal of Animal Science 35, 1102.Google Scholar
Das, T. K. & Waterlow, J. C. (1974). The rate of adaptation of urea cycle enzymes, aminotransferases and glutamic dehydrogenase to changes in dietary protein intake. British Journal of Nutrition 32, 353–373.Google Scholar
Heine, W., Wutzke, K., Richter, F. W. & Plath, C. (1987). Evidence of colonic absorption of protein nitrogen in infants. Acta Paediatrica Scandinavica 76, 741–744.CrossRefGoogle ScholarPubMed
Henin, S. & Smith, M. W. (1976). Electrical properties of pig colonic mucosa measured during early post-natal development. Journal of Physiology 262, 169–187.Google Scholar
Holdsworth, C. D. & Hastings-Wilson, T. (1967). Development of active sugar and amino acid transport in the yolk sac and intestine of the chicken. American Journal of Physiology 212, 233–240.Google Scholar
Hoover, W. H. & Heitmann, R. N. (1975). Caecal nitrogen metabolism and amino acid absorption in the rabbit. Journal of Nutrition 105, 245–252.CrossRefGoogle ScholarPubMed
James, P. S. & Smith, M. W. (1976). Methionine transport by pig colonic mucosa measured during early post- natal development. Journal of Physiology 262, 151–168.Google Scholar
Jarvis, L. G., Morgan, G., Smith, M. W. & Wooding, F. B. P. (1977). Cell replacement and changing transport function in the neonatal pig colon. Journal of Physiology 273, 717–729.CrossRefGoogle ScholarPubMed
Jorgensen, H. & Sauer, W. C. (1982). Amino acid availabilities in protein sources for swine. In The University qf Alberta 61st Annual Feeders' Day Report, pp. 8688. Edmonton, Alberta: University of Alberta Faculty of Extension.Google Scholar
Just, A. (1983). The role of the large intestine in the digestion of nutrients and amino acid utilisation in monogastrics. In Protein Metabolism and Nutrition. Proceedings of the IVth International Symposium, pp. 281–309 [Pion, R., Arnal, M. and Bonin, D., editors]. Paris: Institut National De La Recherch Agronomique.Google Scholar
Just, A, Jorgensen, H. & Fernandez, J. A. (1981). The digestive capacity of the caecum-colon and the value-of the nitrogen absorbed from the hind-gut for protein synThesis in pigs. British Journal of Nutrition 46, 209–219.CrossRefGoogle ScholarPubMed
Larsen, K. (1972). Creatinine assay by a reaction-kinetic principal. Clinica Chimica Acta 41, 209.Google Scholar
Low, A. G. (1980). Nutrient absorption in pigs. Journal of the Science of Food and Agriculture 31, 1087–1130.Google Scholar
McNeil, N. I. (1988). Nutritional implication of human and mammalian large intestinal function. World Review of Nutrition and Dietetics 56, 1–42.CrossRefGoogle ScholarPubMed
Moughan, P. J., Pedraza, M., Smith, W. C., Williams, M. & Wilson, M. (1990). An evaluation with piglets of bovine milk, hydrolysed bovine milk, and isolated soybean proteins included in infant milk formulas. 1. Effect on organ development, digestive enzyme activities, and amino acid digestibility. Journal of Pediatric Gastroentero1ogy and Nutrition 10, 385–394.Google Scholar
Moughan, P. J. & Smith, W. C. (1982). Aspects of protein digestion and absorption in monogastrics with particular reference to dietary formulation. Proceedings of the Nutrition Society of New Zealand 7, 55–65.Google Scholar
Munck, B. G. (1981). Intestinal absorption of amino acids. In Physiology of the Gastrointestinal Tract, Vol. 11, pp. 1097–1122 [Johnson, L. R., editor]. New York: Raven Press.Google Scholar
Niiyama, M., Deguchi, E., Kagota, K. & Namioka, S. (1979). Appearance of 15N-labelled intestinal microbial amino acids in the venous blood of the pig colon. American Journal of Veterinary Research 40, 716–718.Google Scholar
Olszewski, A. & Buraczewski, S. (1978). Absorption of amino acids in isolated pig caecum in situ. Effect of concentration of enzymatic casein hydrolysate on absorption of amino acids. Acta Physiologica Polonica 29, 67–77.Google ScholarPubMed
Potter, G.D. & Lester, R. (1984). The developing colon and nutrition. Journal of Pediutric Gastroenterology and Nutrition 3, 485–487.Google Scholar
Sauer, W. C. & Ozimek, L. (1986). Digestibility of amino acids in swine: results and their practical application. A review. Livestock Production Science 15, 367–388.Google Scholar
Schmitz, M., Ahrens, F., Schon, J. & Hagemeister, H. (1991). Amino acid absorption and its significance for protein supply. In Digestive Physiology in Pigs, pp. 85–87 [Verstegen, M. W. A., Huisman, J., and Den Hartog, L. A., editors]. Wageningen: Pudoc.Google Scholar
Sepulveda, F. V. & Smith, M. W. (1979). Different mechanisms for neutral amino acid uptake by new-born pig colon. Journal of Physiology 286, 479–490.Google Scholar
Smith, M. W. & James, P. S. (1976). Amino acid transport by the helicoidal colon of the new-born pig. Biochimica et Biophysica Acta 419, 391–394.Google Scholar
Tiffany, T. O., Jansen, J. M., Burtis, C. A., Overton, J. B. & Scott, C. D. (1972). Enzymatic kinetic rate and endpoint analyses of substrate by use of a GeMSAEC fast analyser. Clinical Chemistry 18, 829.CrossRefGoogle Scholar
Zebrowska, T. (1973). Digestion and absorption of nitrogenous compounds in the large intestine of pigs. Roczniki Nauk Rolniczych. Series B95, 85–90.Google Scholar
Zebrowska, T. (1975). The apparent digestibility of nitrogen and individual amino acids in the large intestine of pigs. Roczniki Nauk Rolniczych 91, 117–122.Google Scholar
Zebrowska, T. (1978). Apparent digestibility of nitrogen and amino acids and utilization of protein given orally or introduced into the large intestine of pigs. Roczniki Nauk Rolniczych. Series B99, 99–104.Google Scholar
Zebrowska, T. (1982). Nitrogen digestion in the large intestine. In Physiologic Digestive Chez le Porc, pp. 225–236, [Laplace, J. P., Corring, T. and Rerat, A., editors], Paris: Institut National de La Recherche Agronomique.Google Scholar