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Actions of the traditional anti-diabetic plant, Agrimony eupatoria (agrimony): effects on hyperglycaemia, cellular glucose metabolism and insulin secretion

Published online by Cambridge University Press:  22 August 2007

Alison M. Gray  and
Peter R. Flatt
Alison M. Gray*
Affiliation:
School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
Peter R. Flatt
Affiliation:
School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
*
†Corresponding author: Dr Alison M. Gray, fax +44 (0) 1232 382008, email youngheartsproject@hpsspop.n-i.nhs.uk
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Abstract

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Agrimony eupatoria (agrimony) has been documented as a traditional treatment of diabetes. Here, the effects of dietary administration of agrimony on streptozotocin (STZ)-diabetic mice and on in vitro glucose uptake and glucose metabolism, and on insulin secretion by BRIN-BD11 cells were investigated. Agrimony incorporated into the diet (62·5 g/kg) and drinking water (2·5 g/l) countered the weight loss, polydipsia, hyperphagia and hyperglycaemia of STZ-diabetic mice. Aqueous extract of agrimony (1 mg/ml) stimulated 2-deoxy-glucose transport (1·4-fold), glucose oxidation (1·4-fold) and incorporation of glucose into glycogen (2·0-fold) in mouse abdominal muscle comparable with 0·1 μM-insulin. In acute 20 min tests, 0·25-1 mg/ml aqueous extract of agrimony evoked a stepwise 1·9–3·8-fold stimulation of insulin secretion from the BRIN-BD11 pancreatic B-cell line. This effect was abolished by 0·5mM-diazoxide and previous exposure to extract did not adversely affect subsequent stimulation of insulin secretion by 10 mM-L-alanine, thereby indicating that there was no detrimental effect of the extract on cell viability. The effect of extract was glucose-independent and was not evident in BRIN-BD11 cells exposed to a depolarizing concentration of KCl. The ability of agrimony extract to enhance insulin secretion was dependent on use of heat during extract preparation. These results demonstrate the presence of antihyperglycaemic, insulin-releasing and insulin-like activity in Agrimony eupatoria.

Keywords

AgrimonyInsulinDiabetesTraditional plant therapies
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 1 , July 1998 , pp. 109 - 114
Copyright
Copyright © The Nutrition Society 1998

References

Bailey, CJ & Day, C (1989) Traditional treatments for diabetes. Diabetes Care 12, 553–564.CrossRefGoogle ScholarPubMed
Bailey, CJ & Puah, JA (1986) Effect of metformin on glucose metabolism in mouse soleus muscle. Diabète et Metabolisme 12, 212–218.Google ScholarPubMed
Duke, JA (1985) CRC Handbook of Medicinal Herbs, pp. 23–24. Florida: CRC Press.Google Scholar
Dunne, MJ, Harding, EA, Jagger, JH, Ayton, BJ & Squires, PE (1994) Endogenous and chemical activators of ATP-regulated potassium channels in insulin-secreting cells: possible mechanisms and physiological significance. In Frontiers of Insulin Secretion and Pancreatic B-cell Research, pp. 153–159 [Flatt, PR and Lenzen, S, editors]. London: Smith-Gordon.Google Scholar
Eliasson, L, Renström, E, Ämmälä, C , Berggren, P-O, Bertorello, AM, Bokvist, K, Chibalin, A, Deeney, JT, Flatt, PR, Gabel, J, Gromado, J, Larsson, O, Lindström, P, Rhodes, CJ & Rorsman, P (1996) PKC-dependent stimulation of exocytosis by sulfonyl-ureas in pancreatic B cells. Science 271, 813–815.CrossRefGoogle Scholar
Flatt, PR & Bailey, CJ (1981) Abnormal plasma glucose and insulin responses in heterozygous lean (ob/+) mice. Diabetologia 20, 573–577.CrossRefGoogle ScholarPubMed
Flatt, PR, Juntti-Berggren, L, Berggren, P-O, Gould, BJ & Swanston-Flatt, SK (1989) Failure of glucose tolerance factor-containing brewer's yeast to ameliorate spontaneous diabetes in C57/KsJ db/db mice. Diabetes Research 10, 147–151.Google Scholar
Gray, AM & Flatt, PR (1997 a) Nature's own pharmacy: the diabetes perspective. Proceedings of the Nutrition Society 56, 507–517.CrossRefGoogle ScholarPubMed
Gray, AM & Flatt, PR (1997 b) Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant, Medicago saliva (lucerne). British Journal of Nutrition 78, 325–334.CrossRefGoogle Scholar
Hunt SM, Chrzanowska, C, Barnett, CR, Brand, HN & Fawell, JK (1987) A comparison of in vitro cytotoxicity assays and their application to water samples. Alternatives to Laboratory Animals 15, 20–29.Google Scholar
Inoue Y, Tanigawa, K, Nakamura, S, Kato, Y, Tamura, K & Nakase, A (1993) Nicotinamide, a poly (ADP-ribose) synthetase inhibitor, ameliorates B-cell function in partially depancreatized rats. Diabetes Research and Clinical Practice 22, 19–27.Google Scholar
Lewis, JJ (1949) Diabetes and the insulin administration problem. Physiological Reviews 29, 75–90.CrossRefGoogle ScholarPubMed
McClenaghan, NH, Barnet, CR, Ah-Sing E, Abdelwahab, YHA, O'Harte, FPM, Yoon, T-W, Swanston-Flatt, SK & Flatt, PR (1996) Characterization of a novel glucose-responsive insulin-secreting cell line, BRIN-BD11, produced by electrofusion. Diabetes 45, 1132–1140.CrossRefGoogle ScholarPubMed
Okamoto, H (1992) Mechanisms of insulin-secreting cells. In Nutrient Regulation of Insulin Secretion, pp. 387–406 [Flatt, PR, editor]. London: Portland Press.Google Scholar
Prager, R, Schernthaser, G & Graf, H (1986) Effect of metformin on peripheral insulin sensitivity in non-insulin-dependent diabetes mellitus. Diabè;te et Metabolisme 12, 346–350.Google ScholarPubMed
Sandler, S, Hellerstrom, C & Eizirik, DL (1993) Effects of nicotinamide supplementation on human pancreatic-islet function in tissue culture. Journal of Clinical Endocrinology and Metabolism 77, 1574–1576.Google ScholarPubMed
Stevens, JF (1971) Determination of glucose by automatic analyser. Clinica Chimica Ada 32, 199–201.CrossRefGoogle ScholarPubMed
Swanston-Flatt SK, Day, C, Bailey, CJ & Flatt, PR (1990) Traditional plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetologia 33, 462–464.Google Scholar
Swanston-Flatt SK, Day, C, Bailey, CJ & Flatt, PR (1991) Traditional dietary adjuncts for the treatment of diabetes mellitus. Proceedings of the Nutrition Society 50, 641–651.Google Scholar
Toepfer, EW, Mertz, W, Polansky, MM, Roginski, EE & Wolf, WR (1977) Preparation of chromium-containing material of glucose tolerance activity from brewer's yeast extracts and by synthesis. Journal of Agricultural and Food Chemistry 25, 162–166.CrossRefGoogle Scholar
World Health Organization (1980) Second Report of the WHO Expert Committee on Diabetes Mellitus. Technical Report Series no. 646, p. 66. Geneva: WHO.Google Scholar