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Assessment of net postprandial protein utilization of 15N-labelled milk nitrogen in human subjects

Published online by Cambridge University Press:  09 March 2007

Cécile Bos ,
Sylvain Mahé ,
Claire Gaudichon ,
Robert Benamouzig ,
Nicolas Gausserès ,
Catherine Luengo ,
Françoise Ferrière ,
Jacques Rautureau  and
Daniel Tomé
Cécile Bos
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Sylvain Mahé*
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Claire Gaudichon
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Robert Benamouzig
Affiliation:
Service d'hépato-gastroentérologie,Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Nicolas Gausserès
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Catherine Luengo
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Françoise Ferrière
Affiliation:
Service de Biochimie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Jacques Rautureau
Affiliation:
Service d'hépato-gastroentérologie,Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Daniel Tomé
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
*
*Corresponding author: Dr Sylvain Mahé, fax +33 1 44 08 18 25, email mahe@inapg.inra.fr
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Abstract

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The nutritional quality of milk proteins, evaluated both in terms of digestibility and postprandial oxidation and retention in human subjects, was investigated in this study. Five healthy adult volunteers were given 480 ml 15N-labelled milk (i.e. 190 mmol N). 15N was subsequently determined at the ileal level, using a naso-intestinal intubation technique, as well as at the faecal level. Plasma and urine were sampled for 8 h after meal ingestion. Dietary exogenous N recovered at the terminal ileum after 8 h reached 8·6 (se 0·8) mmol while the amount collected in the faeces was 6·5 (se 0·7) mmol after 5 d. The true ileal and faecal digestibilities were 95·5 (se 0·4)% and 96·6 (se 0·4)% respectively. The appearance of [15N]amino acids in the plasma was rapid and prolonged. The measurement of 15N in the body urea pool and in the N excreted in the urine allowed us to calculate the deamination occurring after [15N]milk protein absorption. The net postprandial protein utilization (i.e. NPPU = (Nabsorbed - Ndeaminated)/Ningested), calculated as an index of protein quality 8 h after milk ingestion, was 81·0 (se 1·9)%. Our data confirm that milk protein has a high oro-ileal digestibility in man and demonstrate that milk protein has a high NPPU, an index corresponding to a period in which the dietary protein retention is maximal.

Keywords

MilkProtein qualityIntestinal absorption
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 3 , March 1999 , pp. 221 - 226
Copyright
Copyright © The Nutrition Society 1999

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