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Degradation of hemicellulose and pectin by horse caecum contents

Published online by Cambridge University Press:  09 March 2007

A. Bonhomme-Florentin
A. Bonhomme-Florentin
Affiliation:
L.A. CNRS n° 138, Groupe de Zoologie et Protistologie, les Cézeaux, BP 45, 63170 Aubiere, and Laboratoire de Zoologie, U.F.R. Sciences, BP 347, 51062 Reims-Cédex, France
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Abstract

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1. Polysaccharide depolymerases and glycoside hydrolases involved in the breakdown of plant structural polysaccharides (hemicellulose and pectins) were monitored in three fractions of the liquid phase of horse caecum digesta: acellular fluid (AF), bacteria (B) and protozoa plus bacteria (PB).

2. Both bacteria and protozoa were found to be involved in the decomposition of pectic substances, with two enzymic activities: depolymerase (polygalacturonase, EC 3.2.1.15; and pectin lyase, EC 4.2.2.10) and esterase (pectinesterase, EC 3.1.1.11). The activity of the PB fraction was higher than that of B.

3. With hemicellulosic substrates, all three fractions showed a significant xylan endo-1,3-β-xylosidase (EC 3.2.1.32) activity. Mannan was hardly broken down.

4. Galactomannan and arabinogalactan were broken down more extensively by the PB fraction than by the B fraction. Glycosidase activities (xylan 1,4-β-xylosidase, EC 3.2.1.37 and α-L-arabinofuranosidase, EC 3.2.1.55) were also observed.

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 185 - 192
Copyright
Copyright © The Nutrition Society 1988

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