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Depletion of Δ9 desaturase (EC 1.14.99.5) enzyme activity in growing rat during dietary protein restriction

Published online by Cambridge University Press:  09 March 2007

Michel Narce ,
Jean-Pierre Poisson ,
Jacques Belleville  and
Bernard Chanussot
Michel Narce
Affiliation:
Unité de Recherches en Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, BP 138, 21004 Dijon Cédex, France
Jean-Pierre Poisson
Affiliation:
Unité de Recherches en Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, BP 138, 21004 Dijon Cédex, France
Jacques Belleville
Affiliation:
Unité de Recherches en Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, BP 138, 21004 Dijon Cédex, France
Bernard Chanussot
Affiliation:
Unité de Recherches en Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, BP 138, 21004 Dijon Cédex, France
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Abstract

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The effects of protein restriction on Δ9 desaturase (EC 1.14.99.5) activity were studied in growing rats. A control group was fed on a balanced diet (200 g casein/kg; BD) for 28 d. The experimental group was fed on the low-protein diet (20 g casein/kg; LP) for 26 d, then refed the balanced diet (BD-R) for 2 d. Rats were born to and suckled from normally fed dams. The enzyme activity was measured after 2 and 14 d of LP, and 26 d of LP plus 2 d of BD-R, by incubations in vitro of hepatic microsomal pellets with [1-14C]stearic acid. The results indicated a decreased Δ9 desaturase activity after 2 and 14 d of LP of -33 and -43% respectively. Refeeding for 2 d was sufficient to super-repair this activity (+ 66%). The fatty acid composition of total liver lipids and microsomal phosphatidylethanolamines (PE) and phosphatidylcholines (PC) were also investigated; 18:0 decreased in total liver lipids at 14 d of LP, when 18:1n-9 increased. Stearic acid (18:0) increased in PC at 2 d of LP and in PE at 14 d of LP; oleic acid (18:1n-9) did not change. Therefore, it is concluded that a defect occurred in the bioconversion of 18:0 into 18:1n-9 by Δ9 desaturation during protein depletion. As oleic acid is accumulated in total liver lipids during LP, we speculate that this is due to a decreased oxidation or transport of this fatty acid

Keywords

Microsomal desaturationProtein restrictionLiver lipids
Type
Lipid Metabolism
Information
British Journal of Nutrition , Volume 68 , Issue 3 , November 1992 , pp. 627 - 637
Copyright
Copyright © The Nutrition Society 1992

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