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Dietary cholesterol reduces lipoprotein lipase activity in the atherosclerosis-susceptible Bio F1B hamster

Published online by Cambridge University Press:  09 March 2007

Martina A. McAteer ,
David C. Grimsditch ,
Martin Vidgeon-Hart ,
G. Martin Benson  and
Andrew M. Salter
Martina A. McAteer
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
David C. Grimsditch
Affiliation:
Atherosclerosis Department, GlaxoSmithKline Pharmaceuticals, Harlow, Essex CM19 5AW, UK
Martin Vidgeon-Hart
Affiliation:
Safety Assessment Department, GlaxoSmithKline Pharmaceuticals, Welwyn, Hertfordshire AL6 9AR, UK
G. Martin Benson
Affiliation:
Atherosclerosis Department, GlaxoSmithKline Pharmaceuticals, Harlow, Essex CM19 5AW, UK
Andrew M. Salter*
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
*
*Corresponding author: Dr Andrew M. Salter, fax +44 115 951 6122, email Andrew.Salter@nottingham.ac.uk
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Abstract

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We have compared lipoprotein metabolism in, and susceptibility to atherosclerosis of, two strains of male Golden Syrian hamster, the Bio F1B hybrid and the dominant spot normal inbred (DSNI) strain. When fed a normal low-fat diet containing approximately 40 g fat and 0·3 g cholestero/g, triacylglycerol-rich lipoprotein (chylomicron+VLDL) and HDL-cholesterol were significantly higher (P<0·001) in Bio F1B hamsters than DSNI hamsters. When this diet was supplemented with 150 g coconut oil and either 0·5 or 5·0 g cholestero/g, significant differences were seen in response. In particular, the high-cholesterol diet produced significantly greater increases in plasma cholesterol and triacylglycerol in the Bio F1B compared with the DSNI animals (P=0·002 and P<0·001 for cholesterol and triacylglycerol, respectively). This was particularly dramatic in non-fasting animals, suggesting an accumulation of chylomicrons. In a second experiment, animals were fed 150 g coconut oi/g and 5·0 g cholestero/g for 6 and 12 months. Again, the Bio F1B animals showed dramatic increases in plasma cholesterol and triacylglycerol, and this was confirmed as primarily due to a rise in chylomicron concentration. Post-heparin lipoprotein lipase activity was significantly reduced (P<0·001) in the Bio F1B compared with the DSNI animals at 6 months, and virtually absent at 12 months. Bio F1B animals were also shown to develop significantly more (P<0·001) atherosclerosis. These results indicate that, in the Bio F1B hybrid hamster, cholesterol feeding reduces lipoprotein lipase activity, thereby causing the accumulation of chylomicrons that may be associated with their increased susceptibility to atherosclerosis.

Keywords

Lipoprotein lipaseHepatic lipaseDominant spot normal inbred strainBio F1BDietary cholesterolAtherosclerosisHamster
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 3 , March 2003 , pp. 341 - 350
Copyright
Copyright © The Nutrition Society 2003

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