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Dietary whey protein increases liver and skeletal muscle glycogen levels in exercise-trained rats

Published online by Cambridge University Press:  08 March 2007

Masashi Morifuji ,
Kensuke Sakai ,
Chiaki Sanbongi  and
Katsumi Sugiura
Masashi Morifuji*
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Kensuke Sakai
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Chiaki Sanbongi
Affiliation:
Meiji Seika Kaisha Ltd., Health and Bioscience Laboratories and Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
Katsumi Sugiura
Affiliation:
Meiji Seika Kaisha Ltd., SAVAS Sports and Nutrition Laboratory, 5-3-1 Chiyoda, Sakado-shi, Saitama, 350-0289, Japan
*
*Corresponding author: Dr Masashi Morifuji, fax +81 49 284 7598, email marshimo@yahoo.co.jp
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Abstract

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We investigated the effect of different types of dietary protein on glycogen content in liver and skeletal muscle of exercise-trained rats. Twenty-four male Sprague-Dawley rats (approximately 100 g; n 6 per group) were divided into sedentary or exercise-trained groups with each group being fed either casein or whey protein as the source of dietary protein. Rats in the exercised groups were trained during 2 weeks using swimming exercise for 120 min/d, 6 d/week. Exercise training resulted in an increase in the skeletal muscle glycogen content. Furthermore, the whey protein group significantly increased the skeletal muscle glycogen content compared with the casein group. The increase in glycogen content in liver was significantly greater in rats fed the whey protein diet compared with those fed the casein diet. We also found that the whey protein diet increased the activity of liver glucokinase, whereas it decreased the activities of 6-phosphofructokinase and pyruvate kinase compared with the casein diet. However, hepatic total glycogen synthase activity and mRNA expression were similar with the two diets. In the skeletal muscle, whey protein decreased only 6-phosphofructokinase activity compared with casein. Total glycogen synthase activity in the skeletal muscle in the whey protein group was significantly higher than that in the casein group. The present study is the first to demonstrate that a diet based on whey protein may increase glycogen content in liver and skeletal muscle of exercise-trained rats. We also observed that whey protein regulated glycogen metabolism in these two tissues by different mechanisms.

Keywords

Whey proteinGlycogenGlycoregulatory enzymesExercise-trained rats
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 4 , April 2005 , pp. 439 - 445
Copyright
Copyright © The Nutrition Society 2005

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