Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-20T08:53:42.588Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of conjugated linoleic acid and medium-chain fatty acids on transepithelial calcium transport in human intestinal-like Caco-2 cells

Published online by Cambridge University Press:  09 March 2007

Christopher Jewell  and
Kevin D. Cashman
Christopher Jewell
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Ireland
Kevin D. Cashman*
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Ireland Department of Medicine, University College, Cork, Ireland
*
*Corresponding Author: Professor Kevin D. Cashman, fax +353 21 4270 244, email k.cashman@ucc.ie
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Capric (10:0) and lauric (12:0) acid and conjugated linoleic acid (CLA) have been shown to increase paracellular permeability across human intestinal-like Caco-2 cell monolayers. While this has generated interest in terms of improved drug absorption and delivery, little has been done in terms of their potential effect on nutrient transport across the intestinal epithelium. Therefore, the objective of the present study was to investigate the effect of these fatty acids on transepithelial Ca transport in Caco-2 cells. Cells were seeded onto permeable transport membranes and allowed to differentiate, over 21 d, into intestinal-like cell monolayers. Monolayers (n 9 per treatment) were exposed to 0 (control) or 80 μM-10:0, 80 μM-12:0, 80 μM-18:2, 80 μM-CLA (mixed isomers), 80 μM-cis-9,trans-11 CLA or 80 μM-trans-10,cis-12 CLA for 22 d after seeding (chronic effect) or for 24 h before Ca transport studies (acute effect) on day 22. After exposure, transepithelial and transcellular transport of 45Ca, fluorescein transport (a marker of paracellular Ca transport) and transepithelial electrical resistance (TEER, an indicator of permeability) were measured. Overall Ca transport and TEER in Caco-2 cells were unaffected by exposure to any of the fatty acids for 24 h, or to 18:2, CLA blend or cis-9,trans-11 CLA for 22 d. Paracellular (but not total transepithelial and transcellular) Ca transport across Caco-2 cells was significantly increased (P<0·01, by about 1·5-fold relative to the control value) by exposure to 10:0, 12:0 and trans-10,cis-12 CLA for 22 d, suggesting that these non-esterified fatty acids could potentially enhance Ca absorption in vivo.

Keywords

Conjugated linoleic acidCalcium absorptionCaco-2 cells
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 5 , May 2003 , pp. 639 - 647
Copyright
Copyright © The Nutrition Society 2003

References

Anderberg, EK, Lindmark, T & Artursson, P (1993) Sodium caprate elicits dilatations in human intestinal tight junctions and enhances drug absorption by the paracellular route. Pharmaceutical Research 10 857–864.CrossRefGoogle ScholarPubMed
Auchere, D, Tardivel, S, Gounelle, JC, Drueke, T & Lacour, B (1998) Role of transcellular pathway in ileal Ca2+ absorption: stimulation by low-Ca2+ diet. American Journal of Physiology 275 G951–G956.Google ScholarPubMed
Bronner, F (1998) Calcium absorption – a paradigm for mineral absorption. Journal of Nutrition 128 917–920.CrossRefGoogle ScholarPubMed
Bronner, F & Pansu, D (1999) Nutritional aspects of calcium absorption. Journal of Nutrition 129 9–12.CrossRefGoogle ScholarPubMed
Cashman, KD (2002) Calcium intake, calcium bioavailability and bone health. British Journal of Nutrition 87 S169–S177.CrossRefGoogle ScholarPubMed
Chirayath, MV, Gajdzik, L, Hulla, W, Graf, J, Cross, HS & Peterlik, M (1998) Vitamin D increases tight-junction conductance and paracellular Ca2+ transport in Caco-2 cell cultures. American Journal of Physiology 274 G389–G396.Google ScholarPubMed
Colbeau, A & Maroux, S (1978) Integration of alkaline phosphatase in the intestinal brush border membrane. Biochimica et Biophysica Acta 511 39–51.CrossRefGoogle ScholarPubMed
Dallal, GE (1990) PC-Size consultant – A program for sample size determinations. American Statistician 44, 243.CrossRefGoogle Scholar
Duizer, E, Van Der Wulp, C, Versantvoort, CHM & Groten, JP (1998) Absorption enhancement, structural changes in tight junctions and cytotoxicity caused by palmitoyl carnitine in Caco-2 and IEC-18 cells. Journal of Pharmacology and Experimental Therapeutics 287 395–402.Google ScholarPubMed
Edmonson, JM, Armstrong, LS & Martinez, AO (1988) A rapid and simple MTT-based assay for determining drug sensitivity in monolayer cultures. Journal of Tissue Culture Methods 11 15–17.CrossRefGoogle Scholar
Fleet, JC, Bradley, J, Reddy, GS, Ray, R & Wood, RJ (1996) 1α,25-(OH)2-Vitamin D3 analogs with minimal in vivo calcemic activity can stimulate significant transepithelial calcium transport and mRNA expression in vitro. Archives of Biochemistry and Biophysics 329 228–234.CrossRefGoogle Scholar
Fleet, JC & Wood, RJ (1994) Identification of calbindin D-9k mRNA and its regulation by 1,25-dihydroxyvitamin D3 in Caco-2 cells. Archives of Biochemistry and Biophysics 308 171–174.CrossRefGoogle ScholarPubMed
Fleet, JC & Wood, RJ (1999) Specific 1,25(OH)2D3-mediated regulation of transcellular calcium transport in Caco-2 cells. American Journal of Physiology 276 G958–G964.Google Scholar
Giuliano, AR & Wood, RJ (1991) Vitamin D-regulated calcium transport in Caco-2 cells: unique in vitro model. American Journal of Physiology 260 G207–G212.Google ScholarPubMed
Ha, YL, Storkson, J & Pariza, MW (1990) Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research 50 1097–1101.Google ScholarPubMed
Hochman, JH, Fix, JA & LeCluyse, EL (1994) In vitro and in vivo analysis of the mechanism of absorption enhancement by palmitoylcarnitine. Journal of Pharmacology and Experimental Therapeutics 269 813–822.Google ScholarPubMed
Hunt, SM, Chrzanowski, C, Barnett, CR, Brand, HN & Fawell, JK (1987) A comparison of in vitro cytotoxicity assays and their application to water samples. Alternatives to Laboratory Animals 15 20–29.CrossRefGoogle Scholar
Kennefick, S & Cashman, KD (2000) Investigation of an in vitro model for predicting the effect of food components on calcium availability from meals. International Journal of Food Science and Nutrition 51 45–54.Google Scholar
Kruger, MC & Horrobin, DF (1997) Calcium metabolism, osteoporosis and essential fatty acids: a review. Progress in Lipid Research 36 131–151.CrossRefGoogle ScholarPubMed
Lawson, RE, Moss, AR & Givins, DI (2001) The role of dairy products in supplying conjugated linoleic acid to man's diet: a review. Nutrition Research Reviews 14 153–172.CrossRefGoogle ScholarPubMed
Lindmark, T, Kimura, Y & Artursson, P (1998) Absorption enhancement through intracellular regulation of tight junction permeability by medium chain fatty acids in Caco-2 cells. Journal of Pharmacology and Experimental Therapeutics 284 362–369.Google ScholarPubMed
Lindmark, T, Nikkila, T & Artusson, P (1995) Mechanisms of absorption enhancement by medium chain fatty acids in intestinal epithelial Caco-2 cell monolayers. Journal of Pharmacology and Experimental Therapeutics 275 958–964.Google ScholarPubMed
McRoberts, JA, Aranda, R, Riley, N & Kang, H (1990) Insulin regulates the paracellular permeability of cultured intestinal epithelial cell monolayers. Journal of Clinical Investigation 85 1127–1134.CrossRefGoogle ScholarPubMed
Messer, M & Dahlqvist, A (1966) A one-step ultramicro method for the assay of intestinal disaccharidases. Analytical Biochemistry 14 376–392.CrossRefGoogle ScholarPubMed
Mineo, H, Hara, H & Tomita, F (2001) Short-chain fatty acids enhance diffusional Ca transport in the epithelium of the rat cecum and colon. Life Science 69 517–526.CrossRefGoogle ScholarPubMed
Mossman, T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65 55–63.CrossRefGoogle Scholar
National Institutes of Health (1994) Optimal Calcium Intake: NIH Consensus Statement. Bethesda, MD: NIH, 12:4.Google Scholar
Pinto, M, Robine-Leon, S, Appay, MD, Kedinger, M, Triadow, N, Dussaulx, E, Lacroix, B, Simon-Assmann, P, Haffer, K, Fogh, J & Zweibaum, A (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biology of the Cell 47 323–330.Google Scholar
Roche, HM, Terres, AM, Black, IB, Gibney, MJ & Kelleher, D (2001) Fatty acids and epithelial permeability: effect of conjugated linoleic acid in Caco-2 cells. Gut 48 797–802.CrossRefGoogle ScholarPubMed
Snedecor, GW & Cochran, WG (1967) Statistical Methods. Ames, IA: Iowa State University Press.Google Scholar
Svensson, B, Danielsen, M, Staun, M, Jeppesen, L, Noren, O & Sjostrom, H (1978) An amphiphilic form of dipeptidyl peptidase IV from pig small-intestinal brush-border membrane. European Journal of Biochemistry 90 489–498.CrossRefGoogle ScholarPubMed
Tanaka, Y, Taki, T, Sakame, T, Nodia, T, Sezaki, H & Yamashita, S (1995) Characterization of drug transport through tight-junctional pathway in Caco-2 monolayer: Comparison with isolated rat jejunum and colon. Pharmaceutical Research 12 523–528.CrossRefGoogle ScholarPubMed
US Department of Agriculture, Agricultural Research Service (1997) Data tables: Intakes of 19 individual fatty acids: Results from 1994–96. Continuing Survey of Food Intakes by Individuals, ARS Food Surveys Research Group. http://www.barc.usda.gov/bhnrc/foodsurvey/home.htmGoogle Scholar
Ward, PD, Tippin, TK & Thakker, DR (2000) Enhancing paracellular permeability by modulating epithelial tight junctions. Pharmaceutical Science and Technology Today 3 346–358.CrossRefGoogle ScholarPubMed
Yata, N, Higashi, T, Murakami, R, Yamajo, WM, Wu, K, Taki, Y, Saski, Y & Hideshima, Y (1983) A possible mechanism of absorption promoters. Journal of Pharmacabio–Dynamics 6 s–78.Google Scholar
Yee, S (1997) In vitro permeability across Caco-2 cells (colonic) can predict in vivo (small intestinal) absorption in man - fact or myth. Pharmaceutical Research 14 763–766.CrossRefGoogle ScholarPubMed