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Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion

Published online by Cambridge University Press:  09 March 2007

E. R. Ørskow ,
N. A. MacLeod  and
D. J. Kyle
E. R. Ørskow
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
N. A. MacLeod
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. J. Kyle
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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1. Three experiments were conducted to determine the flow of nitrogen through the rumen and abomasum when cows, steers and lambs were totally nourished on volatile fatty acids infused into the rumen.

2. In two dairy cows (650–700 kg) and two large steers (370–405 kg) the daily flow of non-ammonia-N (NAN) from the rumen was 50.7 and 58 mg/kg live weight (W)0.75 respectively.

3. The flows of NAN through the rumen and abomasum in four young steers (240 315 kg) were 85.0 (SE 21.0) and 195 (SE 7.0) mg/kg W0.75 respectively.

4. In the third experiment the effects of altering rumen pH and osmotic pressure on flow of NAN through the rumen and abomasum were investigated in lambs. While rumen pH and osmotic pressure influenced rumen volume and outflow they had no significant effect on NAN flow. The mean values for NAN outflow from the rumen and abomasum were 76 and 181 mg N/kg W0.75 respectively.

5. Abomasal NAN flow increased with increasing abomasal pH. When osmotic pressure was greater than about 330 mosmol/l in the rumen there was a net inflow of water, while below this value there was net loss of water.

6. For all experiments the flow of N both from the rumen and abomasum was highly variable; this has to be considered if a constant value is used for endogenous N in estimating dietary N in the abomasum.

7. With N-free infusion the rumen NHa concentration varied from 50 to 120 mg NHa-N/I.

8. The amino acid composition of rumen and abomasal N was also determined. Relative to tissue Nit contained a higher proportion of cysteine.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 56 , Issue 1 , July 1986 , pp. 241 - 248
Copyright
Copyright © The Nutrition Society 1986

References

REFERENCES

Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Agricultural Research Council (1984). The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Bergner, H., Bergner, V. & Simon, O. (1983). Proceedings of the 4th International Symposium on Protein Metabolism and Nutrition, pp. 339–342. Clermont-Ferrand: INRA.Google Scholar
Clarke, E. M. W., Ellinger, G. M. & Phillipson, A. T. (1965). Proceedings of the Royal Society, Series B 166, 63–79.Google Scholar
Davidson, J., Mathieson, J. & Boyne, A. W. (1970). Analyst, London 95, 181–193.CrossRefGoogle Scholar
Dinsdale, D., Cheng, K.-J., Wallace, R. J. & Goodlad, R. A. (1980). Applied Environmental Microbiology 39, 1059–1061.CrossRefGoogle Scholar
Dobson, A., Sellars, A. F. & Gatewood, V. H. (1976). American Journal of Physiology 231, 1595–1600.CrossRefGoogle Scholar
Downes, A. M. & McDonald, I. W. (1964). British Journal of Nutrition 18, 1–11.CrossRefGoogle Scholar
Harrop, C. J. F. (1974). Journal of Agricultural Science, Cambridge 83, 237–247.CrossRefGoogle Scholar
Hovell, F. D. DeB., Ørskov, E. R., MacLeod, N. A. & McDonald, I. (1983). British Journal of Nutrition 50, 331&343.CrossRefGoogle Scholar
Institut National de la Recherche Agronomique (1978). La Vache laitière. Theix, France: CRZV, INRA.Google Scholar
Low, A. G. & Rainbird, A. L. (1983). Proceedings of the 4th International Symposium on Protein Metabolism and Nutrition, pp. 343–346. Clermont-Ferrand: INRA.Google Scholar
MacLeod, N. A. (1983). Research in Veterinary Science 34, 122–124.CrossRefGoogle Scholar
MacLeod, N. A., Corrigall, W., Stirton, R. A. & Ørskov, E. R. (1982). British Journal of Nutrition 47, 547–552.CrossRefGoogle Scholar
Malawar, S. J. & Powell, D. P. (1967). Gastroenterology 53, 250–256.CrossRefGoogle Scholar
Mathieson, J. (1970). Proceedings of the Nutrition Society 29, 30A.Google Scholar
Ørskov, E. R. (1982). Protein Nutrition in Ruminants. London and New York: Academic Press.Google Scholar
Ørskov, E. R., Grubb, D. A., Wenham, G. & Corrigall, W. (1979). British Journal of Nutrition 43, 553–558.CrossRefGoogle Scholar
Ørskov, E. R. & MacLeod, N. A. (1982 a). British Journal of Nutrition 47, 625–636.CrossRefGoogle Scholar
Ørskov, E. R. & MacLeod, N. A. (1982 b). Proceedings of the Nutrition Society 41, 76A.Google Scholar
Ørskov, E. R. & MacLeod, N. A. (1983). Proceedings of the Nutrition Society 42, 61A.Google Scholar
Sperber, I., Hyden, S. & Ekman, J. (1957). Kungliga Lantbrugshogskolans Annaler 20, 337–344.Google Scholar
Stacy, B. D. & Warner, A. C. I. (1972). Comparative Biochemical Physiology 43, 637–641.CrossRefGoogle Scholar
Stevenson, A. E. & de Langen, H. (1960). New Zealand Journal of Agricultural Research 3, 314–319.CrossRefGoogle Scholar
Storm, E., Ørskov, E. R. & Smart, R. I. (1984). British Journal of Nutrition 50, 471–478.CrossRefGoogle Scholar
Wallace, R. J., Cheng, K.-J., Dinsdale, D. & Ørskov, E. R. (1979). Nature 279, 424–426.CrossRefGoogle Scholar
Whitehead, R., Cooke, G. H. & Chapman, B. F. (1967). Automation in Analytical Chemistry, Technicon Symposium, vol. 2, p. 377. Basingstoke, Hants: Technicon Instruments Co. Ltd.Google Scholar