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Influence of ascorbic acid supplementation on copper metabolism in rats

Published online by Cambridge University Press:  09 March 2007

Gerrit J. Van Den Berg  and
Anton C. Beynen
Gerrit J. Van Den Berg
Affiliation:
Interfaculty Reactor Institute, Delft University of Technology, Mekelweg 15, 2629 JB Delft, The Netherlands
Anton C. Beynen
Affiliation:
Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht and Department of Human Nutrition, Agricultural University, PO Box 8129, 14 6700 EV Wageningen, The Netherlands
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Abstract

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An attempt was made to unravel further the mechanism by which high dietary concentrations of ascorbic acid influence copper metabolism. The addition of ascorbic acid to the diet of rats caused about a twofold increase in plasma ascorbate concentrations and reduced group mean plasma and tissue concentrations of Cu. The effect of 10 g ascorbic acid/kg diet was greater than that of 1 g/kg. Ascorbic acid feeding reduced blood haemoglobin concentrations and packed cell volume values. Dietary ascorbic acid caused a significant decrease in apparent Cu absorption from the intestine. Ascorbate, intravenously administered together with 64Cu, caused an increase in 64Cu in the liver. Ascorbate, at concentrations occurring in plasma after ascorbic acid feeding, promoted the uptake of 64Cu by isolated hepatocytes. Thus, ascorbate stimulated the efficiency of hepatic uptake of Cu. Ascorbate, intravenously administered together with 64Cu, stimulated accumulation of 64Cu in bile of rats with a bile duct cannula. In rats fed on ascorbic acid, intravenously administered 64Cu was recovered in bile at increased rates. Dietary ascorbic acid enhanced the recovery of intraperitoneally administered 64Cu in faeces. The ascorbate-induced stimulation of biliary 64Cu excretion may reflect an increased hepatic uptake of 64Cu and be caused by an increased specific activity of Cu in liver pools. It is suggested that dietary ascorbic acid reduces tissue Cu concentrations primarily by interfering with intestinal Cu absorption. Ascorbate increases the efficiency of hepatic uptake of Cu, but this effect may not be causatively related with the reduced tissue Cu concentrations after ascorbic acid feeding

Keywords

Tissue copperVitamin CCoppermetabolismAnaemiaRat
Type
Micronutrient Metabolism and Interactions
Information
British Journal of Nutrition , Volume 68 , Issue 3 , November 1992 , pp. 701 - 715
Copyright
Copyright © The Nutrition Society 1992

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