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Influence of feed intake and starvation on the magnitude of Na+,K+-ATPase(EC 3.6.1.3)-dependent respiration in duodenal mucosa of sheep

Published online by Cambridge University Press:  24 July 2007

B. W. McBride  and
L. P. Milligan
B. W. McBride
Affiliation:
Department of Animal Science, The University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
L. P. Milligan
Affiliation:
Department of Animal Science, The University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
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Abstract

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1. Oxygen consumption and Na+,K+-ATPase(EC 3.6.1.3)-dependent (ouabain-sensitive) and -independent respiration were measured for duodenal mucosa biopsies from 10-month-old sheep given two levels of digestible energy (DE) intake (7.6–7.7 and 14.8 MJ lucerne (Medicago sativa) pellets/d) and following 48 h of starvation.

2. The mucosal biopsies were determined to be structurally intact and free of adherent bacteria on histological and scanning-electron-microscope examinations.

3. The use of D-glucose as a substrate during incubations did not elevate (P > 0.05) the respiration indices of the biopsies over those measured during acetate incubations.

4. Glucose uptake did not (P > 0.05) influence the Na+,K+-ATPase-dependent respiration of the mucosal biopsies.

5. Na+,K+-ATPase-dependent respiration accounted for 50% of the total O2, consumption of the mucosal biopsies of sheep given the lower level of DE.

6. Total O2, consumption of the duodenalmucosa was not (P > 0.05) increased when sheep were given the higher level of DE but Na+,K+-ATPase-dependent respiration of the mucosa was elevated (P < 0.01) by 37% during this period.

7. When sheep were starved for 48 h, total O2, consumption of the mucosal biopsies was not (P > 0.05) affected, however, Na+,K+-ATPase-dependent respiration of the biopsies dropped (P < 0.01) by 45%.

8. Na+,K+-ATPase-dependknt respiration accounted for 61.3% of the O2, uptakes of mucosa from the sheep given the higher level of DE and 28.3% of the 02, uptake of mucosa from fasted sheep.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 53 , Issue 3 , May 1985 , pp. 605 - 614
Copyright
Copyright © The Nutrition Society 1985

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