Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-04T05:46:35.021Z Has data issue: false hasContentIssue false
  • English
  • Français

Lycopene in serum, skin and adipose tissues after tomato-oleoresin supplementation in patients undergoing haemorrhoidectomy or peri-anal fistulotomy

Published online by Cambridge University Press:  09 March 2007

Yossi Walfisch ,
S. Walfisch ,
R. Agbaria ,
J. Levy  and
Y. Sharoni
Yossi Walfisch
Affiliation:
Department of Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel Soroka Medical Centre of Kupat Holim, Beer-Sheva, Israel
S. Walfisch
Affiliation:
Soroka Medical Centre of Kupat Holim, Beer-Sheva, Israel The Colorectal Unit, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
R. Agbaria
Affiliation:
Soroka Medical Centre of Kupat Holim, Beer-Sheva, Israel Department of Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
J. Levy
Affiliation:
Department of Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel Soroka Medical Centre of Kupat Holim, Beer-Sheva, Israel
Y. Sharoni*
Affiliation:
Department of Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel Soroka Medical Centre of Kupat Holim, Beer-Sheva, Israel
*
*Corresponding author: Dr Yoav Sharoni, fax +972 8 6403177, email yoav@bgumail.bgu.ac.il
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Lycopene, the main carotenoid found in tomatoes and tomato-based products, has been reported to be protective against several types of cancer. Assessment of changes in plasma concentration of carotenoids following ingestion of lycopene-rich food sources does not necessarily predict changes in lycopene concentration or distribution of its isomers in other body tissues. Our aim was to determine the relationship between concentrations of lycopene and other tomato carotenoids in human serum and body tissues after tomato-oleoresin supplementation. Tomato lycopene oleoresin (30 mg/d) or a placebo was administered for 1 to 7 weeks to seventy-five volunteers undergoing elective haemorrhoidectomy or peri-anal fistulotomy. Carotenoid concentration and isomer distribution in blood and in the surgically removed skin and adipose tissues was measured by HPLC. The serum concentration of lycopene increased after supplementation from 0·26 (sd 0·12) to 0·52 (sd 0·25) μmol/l (n 35; P<0·0001). In the placebo group (n 40), lycopene serum concentration did not change significantly. Serum lycopene concentration after treatment was 2·2-fold greater in the lycopene group than in the placebo group, a slightly higher ratio than that found in skin and adipose tissue (1·6- and 1·4-fold higher than the placebo, respectively). A significant correlation between serum and tissue concentrations was found for both β-carotene and lycopene in the placebo group, whereas in the lycopene-supplemented group the correlation between serum and tissues remained the same for β-carotene but for lycopene was weak. Lycopene supplementation did not significantly change the proportion of all-trans v. cis isomers in the serum and tissues, despite the fact that more than 90 % of the supplemented lycopene was in the all-trans form. These results show that tomato-oleoresin supplementation increases lycopene concentrations in serum and in adipose tissue and skin. The ability to increase lycopene levels in tissues is one of the prerequisites for using it as a food supplement with health benefits.

Keywords

LycopeneCarotenoidsBioavailabilityTomato-oleoresin supplementation
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 4 , October 2003 , pp. 759 - 766
Copyright
Copyright © The Nutrition Society 2003

References

Agarwal, S & Rao, AV (1998) Tomato lycopene and low density lipoprotein oxidation: a human dietary intervention study. Lipids 33, 981–984.CrossRefGoogle ScholarPubMed
Ben-Dor, A, Nahum, A, Danilenko, M, et al. (2001) Effects of acyclo-retinoic acid and lycopene on activation of the retinoic acid receptor and proliferation of mammary cancer cells. Arch Biochem Biophys 391, 295–302.CrossRefGoogle ScholarPubMed
Bohm, V & Bitsch, R (1999) Intestinal absorption of lycopene from different matrices and interactions to other carotenoids, the lipid status, and the antioxidant capacity of human plasma. Eur J Nutr 38, 118–125.Google ScholarPubMed
Boileau, AC, Merchen, NR, Wasson, K, Atkinson, CA & Erdman, JW Jr (1999) Cis-lycopene is more bioavailable than translycopene in vitro and in vivo in lymph-cannulated ferrets. J Nutr 129, 1176–1181.CrossRefGoogle ScholarPubMed
Bowen, PE, Garg, V, Stacewicz-Sapuntzakis, M, Yelton, L & Schreiner, RS (1993) Variability of serum carotenoids in response to controlled diets containing six servings of fruits and vegetables per day. Ann N Y Acad Sci 691, 241–243.Google Scholar
Brown, ED, Micozzi, MS, Craft, NE, et al. (1989) Plasma carotenoids in normal men after a single ingestion of vegetables or purified b-carotene. Am J Clin Nutr 49, 1258–1265.Google Scholar
Chen, L, Stacewicz-Sapuntzakis, M, Duncan, C, et al. (2001) Oxidative DNA damage in prostate cancer patients consuming tomato sauce-based entrees as a whole-food intervention. J Natl Cancer Inst 93, 1872–1879.CrossRefGoogle ScholarPubMed
Edwards, AJ, Vinyard, BT, Wiley, ER, et al. (2003) Consumption of watermelon juice increases plasma concentrations of lycopene and beta-carotene in humans. J Nutr 133, 1043–1050.CrossRefGoogle ScholarPubMed
Franceschi, S, Bidoli, E, La Vecchia, C, Talamini, R, D'Avanzo, B & Negri, E (1994) Tomatoes and risk of digestive-tract cancers. Int J Cancer 59, 181–184.CrossRefGoogle ScholarPubMed
Gartner, C, Stahl, W & Sies, H (1997) Lycopene is more bioavailable from tomato paste than from fresh tomatoes. Am J Clin Nutr 66, 116–122.Google Scholar
Giovannucci, E (1999) Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J Natl Cancer Inst 91, 317–331.Google Scholar
Giovannucci, E, Ascherio, A, Rimm, EB, Stampfer, MJ, Colditz, GA & Willett, WC (1995) Intake of carotenoids and retinol in relation to risk of prostate cancer. J Natl Cancer Inst 87, 1767–1776.CrossRefGoogle ScholarPubMed
Holloway, DE, Yang, M, Paganga, G, Rice-Evans, CA & Bramley, PM (2000) Isomerization of dietary lycopene during assimilation and transport in plasma. Free Radic Res 32, 93–102.CrossRefGoogle ScholarPubMed
Johnson, EJ, Qin, J, Krinsky, NI & Russell, RM (1997) Ingestion by men of a combined dose of beta-carotene and lycopene does not affect the absorption of beta-carotene but improves that of lycopene. J Nutr 127, 1833–1837.CrossRefGoogle Scholar
Karas, M, Amir, H, Fishman, D, et al. (2000) Lycopene interferes with cell cycle progression and insulin-like growth factor I signaling in mammary cancer cells. Nutr Cancer 36, 101–111.CrossRefGoogle ScholarPubMed
Khachik, F, Spangler, CJ, Smith, JC Jr, Canfield, LM, Steck, A & Pfander, H (1997) Identification, quantification, and relative concentrations of carotenoids and their metabolites in human milk and serum. Anal Chem 69, 1873–1881.CrossRefGoogle ScholarPubMed
Kobayashi, T, Itjima, K, Mitamura, T, Torilzuka, K, Cyong, J & Nagasawa, H (1996) Effect of lycopene, a carotenoid, on intrathymic T cell differentiation and peripheral CD4/CD8 ratio in a high mammary tumor strain of SHN retired mice. Anticancer Drugs 7, 195–198.Google Scholar
Kohlmeier, L, Kark, JD, GomezGracia, E, et al. (1997) Lycopene and myocardial infarction risk in the EURAMIC Study. Am J Epidemiol 146, 618–626.CrossRefGoogle ScholarPubMed
Kucuk, O, Sarkar, FH, Sakr, W, et al. (2001) Phase II randomized clinical trial of lycopene supplementation before radical prostatectomy. Cancer Epidemiol Biomarkers Prev 10, 861–868.Google ScholarPubMed
Levy, J, Bosin, E, Feldman, B, et al. (1995) Lycopene is a more potent inhibitor of human cancer cell proliferation than either α-carotene or β-carotene. Nutr Cancer 24, 257–267.Google Scholar
Michaud, DS, Feskanich, D, Rimm, EB, et al. (2000) Intake of specific carotenoids and risk of lung cancer in 2 prospective US cohorts. Am J Clin Nutr 72, 990–997.Google Scholar
Micozzi, MS, Brown, ED, Edwards, BK, et al. (1992) Plasma carotenoid response to chronic intake of selected foods and beta-carotene supplements in men. Am J Clin Nutr 55, 1120–1125.CrossRefGoogle ScholarPubMed
Nagasawa, H, Mitamura, T, Sakamoto, S & Yamamoto, K (1995) Effect of lycopene on spontaneous mammary tumor development in SHN virgin mice. Anticancer Res 15, 1173–1178.Google Scholar
Nahum, A, Hirsch, K, Danilenko, M, et al. (2001) Lycopene inhibition of cell cycle progression in breast and endometrial cancer cells is associated with reduction in cyclin D levels and retention of p27(Kip1) in the cyclin E-cdk2 complexes. Oncogene 20, 3428–3436.Google Scholar
Olmedilla, B, Granado, F, Southon, S, et al. (2002) A European multicentre, placebo-controlled supplementation study with alpha-tocopherol, carotene-rich palm oil, lutein or lycopene: analysis of serum responses. Clin Sci (Lond) 102, 447–456.CrossRefGoogle ScholarPubMed
Paetau, I, Khachik, F, Brown, ED, et al. (1998) Chronic ingestion of lycopene-rich tomato juice or lycopene supplements significantly increases plasma concentrations of lycopene and related tomato carotenoids in humans. Am J Clin Nutr 68, 1187–1195.CrossRefGoogle ScholarPubMed
Paetau, I, Rao, D, Wiley, ER, Brown, ED & Clevidence, BA (1999) Carotenoids in human buccal mucosa cells after 4 wk of supplementation with tomato juice or lycopene supplements. Am J Clin Nutr 70, 490–494.CrossRefGoogle ScholarPubMed
Parker, RS (1989) Carotenoids in human blood and tissues. J Nutr 119, 101–104.Google Scholar
Pellegrini, N, Riso, P & Porrini, M (2000) Tomato consumption does not affect the total antioxidant capacity of plasma. Nutrition 16, 268–271.CrossRefGoogle Scholar
Porrini, M & Riso, P (2000) Lymphocyte lycopene concentration and DNA protection from oxidative damage is increased in women after a short period of tomato consumption. J Nutr 130, 189–192.CrossRefGoogle Scholar
Porrini, M, Riso, P & Oriani, G (2002) Spinach and tomato consumption increases lymphocyte DNA resistance to oxidative stress but this is not related to cell carotenoid concentrations. Eur J Nutr 41, 95–100.CrossRefGoogle Scholar
Re, R, Fraser, PD, Long, M, Bramley, PM & Rice-Evans, C (2001) Isomerization of lycopene in the gastric milieu. Biochem Biophys Res Commun 281, 576–581.CrossRefGoogle ScholarPubMed
Richelle, M, Bortlik, K, Liardet, S, et al. (2002) A food-based formulation provides lycopene with the same bioavailability to humans as that from tomato paste. J Nutr 132, 404–408.CrossRefGoogle ScholarPubMed
Stahl, W, Schwarz, W, Sundquist, AR & Sies, H (1992) cis-trans isomers of lycopene and beta-carotene in human serum and tissues. Arch Biochem Biophys 294, 173–177.Google Scholar
Stahl, W & Sies, H (1992) Uptake of lycopene and its geometrical isomers is greater from heat-processed than from unprocessed tomato juice in humans. J Nutr 122, 2161–2166.Google Scholar
Stahl, W, Sundquist, AR, Hanusch, M, Schwarz, W & Sies, H (1993) Separation of beta-carotene and lycopene geometrical isomers in biological samples. Clin Chem 39, 810–814.Google Scholar
van Breemen, RB, Xu, X, Viana, MA, et al. (2002) Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce. J Agric Food Chem 50, 2214–2219.CrossRefGoogle ScholarPubMed
Wingerath, T, Sies, H & Stahl, W (1998) Xanthophyll esters in human skin. Arch Biochem Biophys 355, 271–274.Google Scholar
Zhang, L-X, Cooney, RV & Bertram, J (1991) Carotenoids enhance gap junctional communication and inhibit lipid peroxidation in C3H/10T1/2 cells: relationship to their cancer chemopreventive action. Carcinogenesis 12, 2109–2114.Google Scholar
Zhang, LX, Cooney, RV & Bertram, JS (1992) Carotenoids up-regulate connexin-43 gene expression independent of their provitamin-A or antioxidant properties. Cancer Res 52, 5707–5712.Google ScholarPubMed
Zhang, SM, Tang, GW, Russell, RM, Mayzel, KA, Stampfer, MJ, Willett, WC & Hunter, DJ (1997) Measurement of retinoids and carotenoids in breast adipose tissue and a comparison of concentrations in breast cancer cases and control subjects. Am J Clin Nutr 66, 626–632.CrossRefGoogle Scholar