Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-25T01:38:55.923Z Has data issue: false hasContentIssue false
  • English
  • Français

Measurement of 3-methylhistidine production in lambs by using compartmental-kinetic analysis*

Published online by Cambridge University Press:  09 March 2007

J. A. Rathmacher ,
G. Link  and
S. Nissen
J. A. Rathmacher
Affiliation:
Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
G. Link
Affiliation:
Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
S. Nissen
Affiliation:
Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The kinetics of 3-methylhistidine (3MH) metabolism in four crossbred lambs were studied. Each lamb was injected with an intravenous dose of 3-[2H3]methylhistidine (d3-3MH) and the stable isotope disappearance in plasma and appearance in both urine and muscle were measured. Immediately after the administration of tracer there was a phase of rapid disappearance of tracer from the plasma, which was followed by a more gradual decrease in d3-3MH from the plasma during the last 4 d of the experiment. A minimum of three exponentials was required to describe the plasma decay curve adequately. The kinetic model of 3MH in the whole animal was constructed by using the SAAM/CONSAM computer modelling program. Two different configurations of a three-compartment model are described: (1) a simple three-pool model, in which plasma kinetics were entered into pool 1 out of which they had one undefinable exit; (2) a plasma-urinary three-pool model with two exits, in which the urinary kinetics were entered as an exit out of pool 1 and required a second exit out of pool 3 to produce an adequate fit. In addition, muscle kinetics from biopsies of the longissirnus dorsi were entered into either pool 2 or 3 using the plasma-urinary model. Steady-state mass and transport rate values were obtained for each model construct described, and a de novo production rate corresponding to a fractional breakdown rate of myofibrillar protein of approximately 5%/d was also calculated. The model predicted that only 15% of 3MH was excreted in urine as free 3MH, which is consistent with current knowledge of 3MH excretion in sheep. The simple three-pool plasma kinetic model, therefore, could be used to estimate, by a relatively simple injection-sampling technique, the extent of muscle protein turnover in lambs.

Keywords

3-MethylhistidineKinetic modelLamb
Type
Nitrogen Metabolism
Information
British Journal of Nutrition , Volume 69 , Issue 3 , May 1993 , pp. 743 - 755
Copyright
Copyright © The Nutrition Society 1993

References

Berman, M. & Weiss, M. F. (1978). SAAM Manual. US Department of Health, Education and Welfare Publication no. (NIH) 78–180. Washington, DC: US Government Printing Office.Google Scholar
Boston, R. C., Grief, P. C. & Berman, M. (1981). Conversational SAAM – an inter-reactive program for kinetic analysis of biological systems. Computer Programs in Biomedicine 13, 111–119.CrossRefGoogle Scholar
Garlick, P. J. (1980). Assessment of protein metabolism in the intact animal. In Protein Deposition in Animals [Buttery, P. J. and Lindsay, D. B., editors]. London: Butterworths.Google Scholar
Harris, C. I. (1981). Reappraisal of the quantitative importance of non-skeletal-muscle source of N-tau-methylhistidine in urine. Biochemical Journal 194, 1011–1014.CrossRefGoogle ScholarPubMed
Harris, C. I. & Milne, G. (1980). The urinary excretion of Nτ-methyl histidine in sheep: an invalid index of muscle protein breakdown. British Journal of Nutrition 44, 129–140.CrossRefGoogle Scholar
Harris, C. I. & Milne, G. (1981). The urinary excretion of Nτ-methyl histidine by cattle: validation as an index of muscle protein breakdown. British Journal qf Nutrition 45, 411–422.CrossRefGoogle Scholar
Harris, C. I. & Milne, G. (1987). The identification of the N-methyl histidine-containing dipeptide, balenine, in muscle extracts from various mammals and the chicken. Comparative Biochemical Physiology 86B, 273–279.Google Scholar
Harris, C. I., Milne, G., Lobley, G. E. & Nicholas, G. A. (1977). 3-Methylhistidine as a measure ofskeletal-muscle protein catabolism in the adult New Zealand white rabbit. Biochemical Society Transactions 5, 706–708.CrossRefGoogle ScholarPubMed
Johnson, P., Harris, C. I. & Perry, S. V. (1967). 3-Methylhistidine in actin and other muscle proteins. Biochemical Journal 105, 361–370.CrossRefGoogle ScholarPubMed
Millward, D. J. & Bates, P. C. (1983). 3-Methylhistidine turnover in the whole body, and the contribution of skeletal muscle and intestine to urinary 3-methylhistidine excretion in the adult rat. Biochemical Journal 214, 607–615.CrossRefGoogle ScholarPubMed
National Research Council (1985). Nutrient Requirements of Sheep. Washington, DC: National Academy Press.Google Scholar
Nishizawa, N., Noguchi, T. & Hareyama, S. (1977). Fractional flux rates of Nτ-methylhistidine in skin and gastrointestine: the contribution of these tissues to urinary excretion of Nτ-methylhistidine in the rat. British Journal of Nutrition 38, 149–151.CrossRefGoogle ScholarPubMed
Young, V. R., Alex, S. D., Baliga, B. S., Munro, H. N. & Muecke, W. (1972). Metabolism of administered 3-methylhistidine: lack of muscle transfer ribonucleic acid charging and quantitative excretion as 3-methylhistidine and its N-acetyl derivative. Journal of Biological Chemistry 217, 3592–3600.CrossRefGoogle Scholar
Young, V. R., Haverberg, L. N., Bilmazes, C. & Munro, H. N. (1973). Potential use of 3-methylhistidine excretion as an index of progressive reduction in muscle protein catabolism during starvation. Metabolism 22, 1429–1436.CrossRefGoogle Scholar
Young, V. R. & Munro, H. N. (1978). Nt-Methylhistidine (3-methylhistidine) and muscle protein turnover: An overview. Federation Proceedings 31, 2291–2300.Google Scholar