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Protein and enery relationships in the broiler chicken 12. Dietary protein and triidothyronine (T3) effects on the response of broilers to isoproterenol and cyclic adenosine monophosphate in vitro*

Published online by Cambridge University Press:  09 March 2007

R.W. Rosebrough  and
A.D. Mitchell
R.W. Rosebrough
Affiliation:
Nonruminant Animal Nutrition Laboratory, Livestock and Poultry Science Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
A.D. Mitchell
Affiliation:
Nonruminant Animal Nutrition Laboratory, Livestock and Poultry Science Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
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Abstract

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Indian River male broiler chickens (7-d-old) were fed on diets containing 120, 210 or 300 g crude protein/kg + 0 or 1 mg triiodothyronine (T3)/kg diet (Expt 1) and 120, 150, 180 or 210 g crude protein/kg +0 or 1 mg T3/kg diet (Expt 2) to determine the effects of crude protein level and T3 on growth and metabolism. Body composition of chickens was determined by a combination of dissection of muscle and abdominal fat pads, and chemical extraction (Expt 1). In vitro lipogenesis (IVL) was determined in both experiments by incubating liver explants for 2 h at 37° in the presence of 10-4 M-dibutyryl cyclic AMP (cAMP) or 10-5 M isoproterenol (ISO) and 10-2 M-[2-14C]acetate. Acetate incorporation into total lipid was an indication of IVL. Activity ratios for each of these additions relative to control (?cAMP?ISO) were calculated to ascertain basal ν. inhibited rates of IVL. The relative muscle mass was increased by increasing crude protein from 120 to 210 g/kg diet but not from 210 to 300 g/kg diet. Dietary T3 decreased total body lipid regardless of the dietary crude-protein level. Increasing dietary crude protein decreased (P<0.05) basal IVL (?cAMP? ISO) but not IVL (+ cAMP). Dietary T3 decreased basal IVL in birds fed on the diets containing 120 and 210 g crude protein/kg but had little effect on the two inhibited states of lipogenesis (+ cAMP or +ISO). The component of lipogenesis sensitive to in vitro inhibition is also the component under dietary control.

Keywords

Dietary proteinTriiodothyronineLipogenesisChicken
Type
Chickhen liver metabolism in vitro
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 73 - 85
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Bartov, I. (1979) Nutritional factors affecting quantity and quality of carcass fat in chickens. Federation Proceedings 38, 2627–2639.Google ScholarPubMed
Buttery, P. J. & Boorman, K. N. (1976) The energetic efficiency of amino acid metabolism. In Protein Metabolismand Nutrition, pp. 197–204. London: Butterworths.Google Scholar
Campbell, R. M. & Scanes, C. G. (1985) Adrenergic control of lipogenesis and lipolysis in the chicken in vitro. Compurutive Biochemistry and Physiology, 82C, 137–142.Google Scholar
Capuzzi., D. M., Lackman, R. D. & Reed, M. A. (1975) Species differences in the hormonal control of lipogenesis in rat and chicken hepatocytes. Comparative Biochemistry and Physiology 43B, 689–701.Google Scholar
Chiasson, R. B., Sharp, P. J., Klandorf, H., Scanes, C. G. & Harvey, S. (1979) The effect of rapeseed meal and methimazole on levels of plasma hormones in growing broiler cockerels. Poultry Science 58, 1575–1583.CrossRefGoogle ScholarPubMed
Clarke, S. D. & Hembree, J. (1990) Inhibition of triiodothyronine's induction of rat liver lipogenic enzymes by dietary fat Journal of Nutrition 120, 625–630.CrossRefGoogle ScholarPubMed
Clarke, S. D., Watkins, P. A. & Lane, M. D. (1979) Acute control of fatty acid synthesis by cyclic AMP in the chick liver cell: possible site of inhibition of citrate formation. Journal of Lipid Research 20, 974–985.CrossRefGoogle ScholarPubMed
Cleland, W. W., Thompson, V. M. & Barden, R. E. (1969) Isocitrate dehydrogenase (TPN specific) from pig heart. In Methods in Enzymology, vol. 13, pp. 30–33 [Lowenstein, J. M., editor]. New York: Academic Press.Google Scholar
Cogburn, L. A., Liou, S. S., Alfonso, C. P., McGuiness, M. C. & McMurtry, J. P. (1989) Dietary thyrotropin-releasing hormone stimulates growth rate and increases the insulin: glucagon molar ratio of broiler chickens. Proceedings of the Society for Experimental Biology and Medicine 192, 127–134.CrossRefGoogle ScholarPubMed
Cramb, G., Langslow, D. R. & Phillips, J. H. (1982) Hormonal effects on cyclic nucleotides and carbohydrate and lipid metabolism in isolated chicken hepatocytes. General and Comparative Endocrinology 46, 310–321.CrossRefGoogle ScholarPubMed
Decuypere, E., Buyse, J., Scanes, C. G., Huybrechts, L. & Kuhn, E. R. (1987) Effects of hyper- or hypothyroidstatus on growth, adiposity and levels of growth hormone, somatomedin C and thyroid metabolism in broiler chickens. Reproduction, Nutrition Developpement 27, 555–565.CrossRefGoogle ScholarPubMed
Donaldson, W. E. (1985) Lipogenesis and body fat in chicks: effects of calorie: protein ratios and dietary fat. Poultry Science 64, 1199–1204.CrossRefGoogle ScholarPubMed
Donaldson, W. E., Combs, G. F. & Romoser, G. L. (1956) Studies on energy levels in poultry rations. 1. The effect of calorie-protein of the ration on growth, nutrient utilization and body composition of chicks. Poultry Science 35, 1100–1204.CrossRefGoogle Scholar
Dozin, B., Magnuson, M. D. & Nikodem, V. M. (1986) Thyroid hormone regulation of malic enzyme synthesis. Journal of Biological Chemistry 261, 10290–10292.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Granner, D. K. & Tomkins, G. M. (1970) Tyrosine amino transferase. In Methods in Enzymology, vol. 17, pp.633–637 [Lowenstein, J. M., editor]. New York: Academic Press.Google Scholar
Hanks, J. H. & Wallace, R. E. (1949) Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proceedings of the Society for Experimental Biology and Medicine 71, 196–200.CrossRefGoogle ScholarPubMed
Harvey, S. (1983) Thyroid hormones inhibit growth hormone secretion in domestic fowl (Gallus domesticus). Journal of Endocrinology 98, 129–135.CrossRefGoogle Scholar
Hsu, R. Y. & Lardy, H. A. (1969) Malic enzyme. In Methods in Enzymology, vol. 13, pp. 230–235 [Lowenstein, J. M., editor]. New York: Academic Press.Google Scholar
Jump, D. B., Vett, A, Santiago, V., Lepar, G. & Herberholz, L. (1988) Transcriptional activation of rat liver S14 gene during postnatal development. Journal of Biological Chemistry 263, 7254–7261.CrossRefGoogle ScholarPubMed
Lauterio, T. J. & Scanes, C. G. (1987) Hormonal responses to protein restriction in two strains of chickens with different growth characteristics. Journal of Nutrition 117, 758–763.CrossRefGoogle ScholarPubMed
Magnuson, M. A. & Nikodem, V. M. (1983) Molecular cloning of a cDNA sequence for rat malic enzyme. Journal of Biological Chemistry 258, 12712–12717.CrossRefGoogle ScholarPubMed
Mariash, C. N., Kaiser, F. E. & Oppenheimer, J. H. (1980) Comparison of the response characteristics of four lipogenic enzymes to 3,5,3'-triiodothyronine administration- evidence for variable degrees of amplification of the nuclear 3,5,3'-triiodothyronine signal. Endocrinology 106, 22–27.CrossRefGoogle ScholarPubMed
Martin, R. J. & Herbein, J. H. (1976) A comparison of the enzyme levels and in vitro utilization of various substrates for lipogenesis in pair-fed lean and obese pigs. Proceedings of the Society for Experimental Biology and Medicine 151, 231–235.CrossRefGoogle ScholarPubMed
May, J. D. (1980) Effect of dietary thyroid hormone on growth and feed efficiency of broilers.Poultry Science 59, 888–492.CrossRefGoogle ScholarPubMed
Mersmann, H. J., Houk, J. M., Phinney, G., Underwood, M. C. & Brown, L. J. (1973) Lipogenesis by in vitro liver and adipose tissue preparations from neonatal swine. American Journal of Physiology 224, 1123–1129.CrossRefGoogle ScholarPubMed
Nakamura, T., Tomomura, A., Noda, C., Mineko, S. & Ichihara, A. (1983) Acquisition of β-adrenergic response by adult rat hepatocytes during primary culture. Journal of Biological Chemistry 258, 9283–9289.CrossRefGoogle ScholarPubMed
Perez-Castillo, A, Schwartz, H. L. & Oppenheimer, J. H. (1987) Rat hepatic mRNA-S14 and lipogenic enzymes during weaning: role of S14 in lipogenesis. American Journal of Physiology 253, E536–E542.Google ScholarPubMed
Remington, R. D. & Schork, M. A. (1970).Statistics with Applications to the Biological and Health Sciences. Englewood Cliffs, New Jersey: Prentice-Hall.Google Scholar
Roncari, D. A. K. & Murthy, V. K. (1975) Effects of thyroid hormones on enzymes involved in fatty acid and glycerolipid synthesis. Journal of Biological Chemistry 230, 4134–4138.CrossRefGoogle Scholar
Rosebrough, R. W. (1994) Nutritional effects on neural transmitter metabolism in the broiler chicken. Comparative Biochemistry and Physiology 107A, 573–580.CrossRefGoogle Scholar
Rosebrough, R. W. & Jones, R. A. (1992) Effects of the second messenger system on the responses of broilers fed triiodothyronine (T3) and graded levels of crude protein. Poultry Science 71, Suppl. 1, 80.Google Scholar
Rosebrough, R. W., McMurtry, J. P., Mitchell, A. D. & Steele, N. C. (1988) Protein and energy relations in the broiler chicken. 6. Effect of dietary protein and energy restrictions on in vitro carbohydrate and lipid metabolism and metabolic hormone profiles. Comparative Biochemistry and Physiology 90, 311–316.Google Scholar
Rosebrough, R. W., McMurtry, J. P. & Vasilatos-Younken, R. (1991) Effect of pulsatile (P) or continuous (C) administration of pituitary-derived chicken growth hormone (pcGH) on lipid metabolism in broiler pullets. Comparative Biochemistry and Physiology 99A, 207–214.CrossRefGoogle Scholar
Rosebrough, R. W., McMurtry, J. P. & Vasilatos-Younken, R. (1992) Lipid metabolism in liver explants fromhyperthyroid chickens. British Journal of Nutrition 68, 667–676.CrossRefGoogle ScholarPubMed
Rosebrough, R. W. & Steele, N. C. (1985) Energy and protein relations in the broiler. 1. Effect of protein levels and feeding regimes on growth, body composition and in vitro lipogenesis in broiler chickens. Poultry Science 64, 119–126.CrossRefGoogle Scholar
Rosebrough, R. W. & Steele, N. C. (1987) Methods to assess glucose and lipid metabolism in avian liver explants. Comparative Biochemistry and Physiology 88A, 1041–1049.Google Scholar
Scanes, C. G., Duyka, D. R., Lauterio, T. J., Bowen, S. J., Huybrechts, L. M., Bacon, W. L. & King, D. B. (1986) Effect of chicken growth hormone, triiodothyronine and hypophysectomy in growing fowl. Growth 50, 12–31.Google Scholar
Thomas, O. P. & Combs, G. F. (1967) Relationship between serum protein level and body composition in the chick. Journal of Nutrition 91, 468–472.CrossRefGoogle Scholar
Yeh, Y. Y. & Leveille, G. A. (1969) Effect of dietary protein on hepatic lipogenesis in the growing chick. Journal of Nutrition 98, 356–366.CrossRefGoogle ScholarPubMed