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Rat small intestinal morphology and tissue regulatory peptides: effects of high dietary fat

Published online by Cambridge University Press:  09 March 2007

F. A. Sagher ,
J. A. Dodge ,
C. F. Johnston ,
C. Shaw ,
K. D. Buchanan  and
K. E. Carr
F. A. Sagher
Affiliation:
Departments of Child Health, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
J. A. Dodge
Affiliation:
Departments of Child Health, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
C. F. Johnston
Affiliation:
Departments of MedicineThe Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
C. Shaw
Affiliation:
Departments of MedicineThe Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
K. D. Buchanan
Affiliation:
Departments of MedicineThe Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
K. E. Carr
Affiliation:
Departments of Anatomy, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland
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Abstract

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Sprague–Dawley rats (3 weeks old) were fed on isoenergetic diets in which 40 % of the total energy was provided as fat either in the form of butter (high saturated fat), olive oil (high monounsaturated fat) or maize oil (high polyunsaturated fat), with one group on low-fat (10% of total energy) standard diet as a control. Animals were killed after 8.4 (se 0.8) weeks by cardiac puncture. Similar pieces of jejunum and ileum were prepared for morphometric studies. Extracts of tissue from the proximal and distal segments of the whole small intestine from four animals per group were assayed using established techniques for enteroglucagon, motilin, neurotensin, somatostatin, substance P and vasoactive intestinal peptide (VIP). We found that maize oil and olive oil increased villus height: crypt depth ratio in both jejunum and ileum. Maize oil increased tissue concentrations of somatostatin (P < 0.05) and substance P (P < 0.005) in the proximal segment. Both maize oil and olive oil increased tissue concentrations of neurotensin and substance P (P < 0.005) in the distal segments. These observations may explain the improvement of intestinal absorption of fluid following supplementation with polyunsaturated fat

Keywords

Small intestinal morphologyGut peptidesRat
Type
Diet and Gastrointestinal Function
Information
British Journal of Nutrition , Volume 65 , Issue 1 , January 1991 , pp. 21 - 28
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Adrian, T. E. & Bloom, S. R. (1985). Effect of food on gut hormones. In Food and the Gut, pp. 13–29 [Hunter, J. O. and Jones, V. A., editors]. London: Baillière Tindall.Google Scholar
Ardill, J. (1979). Radioimmunoassay of gastrointestinal hormones. In Clinics in Endocrinology and Metabolism, vol. 8:2, pp. 265–280 [Buchanan, K. D., editor]. London: W. B. Saunders.Google Scholar
Brownlee, A. & Moss, A. (1959). Changes in size of the gastrointestinal tract, liver and kidneys of the laboratory rat at different liver weight and on different dietary regimes. British Veterinary Journal 115, 225–234.Google Scholar
Bryant, M. G. & Bloom, S. R. (1982). Measurement in tissues. In Radioimmunoassay of Gut Regulatory Peptides, pp. 38–42 [Bloom, S. R. and Long, R. G., editors]. London: W. B. Saunders.Google Scholar
Carraway, R. & Leeman, S. E. (1976). Characterization of radioimmunoassayable neurotensin in the rat: its differential distribution in the central nervous system, the small intestine and stomach. Journal of Biological Chemistry 251, 7045–7052.CrossRefGoogle ScholarPubMed
Challacombe, D. N., McDonald, D. T. & Wheeler, E. E. (1983). A quantitative assessment of jejunal villous damage in coeliac disease, using the mucosal index. Hepato-Gastroenterology 30, 113–115.Google Scholar
Christofides, N. D., Bloom, S. R., Besterman, H. S., Adrian, T. E. & Ghatie, M. A. (1979). Release of motilin by oral and intravenous nutrients in man. Gut 20, 102–106.Google Scholar
Clarke, R. M. (1975). Diet, mucosal architecture and epithelial cell production in the small intestine of specified-pathogen-free and conventional rats. Laboratory Animals 9, 201–209.Google Scholar
Clarke, R. M. (1977). The effects of age on mucosal morphology and epithelial cell production in rat small intestine. Journal of Anatomy 123, 805–811.Google ScholarPubMed
Creamer, B. (1964). Variations in small intestinal villous shape and mucosal dynamics. British Medical Journal ii, 1371–1373.Google Scholar
Fabry, P. & Kumalove, V. (1960). Enhanced growth of the small intestine in rats as a result of adaptations to intermittent starvation. Acta Anatomica 43, 264–271.CrossRefGoogle ScholarPubMed
Flanagan, R. W. J., Buchanan, K. D. & Murphy, R. F. (1974). Specificity of antibodies in the radioimmunoassay of glucagon. Diabetologia 10, 365.Google Scholar
Ghatei, M. A. & Bloom, S. R. (1981). Enteroglucagon in man. In Gut Hormones, vol. 2, pp. 332–338 [Bloom, S. R. and Polak, J. M., editors]. Edinburgh: Churchill Livingstone.Google Scholar
Grey, L. V., Garofalo, C., Greenberg, G. R. & Morin, C. L. (1984). The adaptation of the small intestine after resection in response to free fatty acids. American Journal of Clinical Nutrition 40, 1235–1242.CrossRefGoogle ScholarPubMed
Hindmarsh, J. T., Kilby, D. & Roff, B. (1967). Further studies on intestinal transport during semi-starvation. Journal of Physiology 188, 207–218.Google Scholar
Hooper, C. S. & Blair, M. (1956). The effect of starvation on epithelial renewal in the rat duodenum. Experimental Cell Research 14, 175–181.Google Scholar
Hopper, A. F., Wannemacher, R. W. & McGovern, A. (1968). Cell population changes in the intestinal epithelium of the rat following starvation and protein depletion. Proceedings of the Society for Experimental Biology and Medicine 128, 695–698.CrossRefGoogle ScholarPubMed
Ito, S., Matsubara, Y. & Shibata, A. (1982). Plasma radioimmunoassayable neurotensin responses to the administration of various nutrients. Tokyo Journal of Experimental Medicine 136, 109–110.Google Scholar
Kapadia, S. & Baker, S. J. (1976). The effects of alterations in villus shape on the intestinal mucosal surface of the albino rat: the relationship between mucosal surface area and crypts. Digestion 14, 256–268.CrossRefGoogle ScholarPubMed
Kihl, B., Rokaeus, A., Rosell, S. & Olbe, L. (1981). Fat inhibition of gastric acid secretion in man and plasma concentrations of neurotensin-like immunoreactivity. Scandinavian Journal of Gastroenterology 16, 513–526.Google Scholar
Long, R. G. & Bryant, M. G. (1982). Vasoactive intestinal polypeptide. In Radioimmunoassay of Gut Regulatory Peptides, pp. 120–130 [Bloom, S. R. and Long, R. G., editors]. London: W. B. Saunders.Google Scholar
McManus, J. P. A. & Isselbacher, K. J. (1970). Effect of fasting versus feeding on the rat small intestine: morphological, biochemical and functional differences. Gastroenterology 59, 214–221.Google Scholar
Miazza, B. M., Al-Mukhtar, M. Y. T. & Salmeron, M. (1985). Hyperenteroglucagonaemia and small intestinal mucosal growth after caloric perfusion of glucose in rats. 26, 518 524.Google Scholar
Nemeroff, C. B., Hernandez, D. E., Orlando, R. C. & Prange, A. J. (1982). Cytoprotective effect of centrally administered neurotensin on stress-induced gastric ulcers. American Journal of Physiology 242, G342–G346.Google ScholarPubMed
Robinson, J. W. L. & Dowling, R. H. (1982). Mechanisms of intestinal adaptations. In Falk Symposium no. 30, pp. 175–184 [Robinson, J. W. L., Dowling, R. H. and Riecken, E. O., editors]. Lancaster: MTP Press.Google Scholar
Rosell, S. (1982). The role of neurotensin in the uptake and distribution of fat. Annals of the New York Academy of Sciences 400, 183–197.Google Scholar
Rosell, S. & Rokaeus, A. (1979). The effect of ingestion of amino acids, glucose and fat on circulating neurotensin-like immuno-reactivity (NTLI) in man. Acta Physiologica Scandinavica 107, 263–267.Google Scholar
Sagher, F. A. (1988). The effects of variation in dietary fat in early life, with particular reference to the small intestine. PhD Thesis, The Queen's University of Belfast.Google Scholar
Sagher, F. A., Dodge, J. A., Moore, R., McMaster, C. & McCaughey, G. (1990). Modulation of fluid absorption and the secretory response of rat jejunum to cholera toxin by dietary fat. Gut 31 (In the Press).CrossRefGoogle ScholarPubMed
Schultzberg, M., Hokfelt, T., Nilsson, G., Terenius L., Rethfeld, J. F., Brown, M., Elde, R., Goldstein, M. & Said, S. (1980). Distribution of peptide and catecholamine-containing neurons in the gastrointestinal tract of rat and guinea-pig. Neuroscience 5, 689–744.Google Scholar
Shaw, C. & Buchanan, K. D. (1983). Intact neurotensin NT in human plasma: response to oral feeding. Regulatory Peptides 7, 145–153.CrossRefGoogle ScholarPubMed
Shaw, C., Watt, P. C. H. & Buchanan, K. D. (1986). Meal stimulated neurotensin immunoreactivity in plasma following gastric surgery: characterisation with two region specific antisera. Digestion 33, 152–160.Google Scholar
Slavin, G., Sowter, C., Robertson, K., McDermott, S. & Patton, K. (1980). Measurement in jejunal biopsies by computer-aided microscopy. Journal of Clinical Pathology 33, 254–261.Google Scholar
Solcia, E., Polak, J. M., Pearse, A. G. E., Forssmann, W. G., Larsson, L.-I., Sundler, F., Leechago, J., Grimelius, L., Fumita, T., Creutzfeldt, W., Gepts, W., Falkmer, S., Lefranc, G., Heitz, P., Hage, E., Buchanan, A. M. J., Bloom, S. R. & Crossman, M. I. (1978). Classification of gastroenteropancreatic endocrine cells. In Gut Hormones, pp. 40–48 [Bloom, S. R. and Polak, J. M., editors]. Edinburgh: Churchill Livingstone.Google Scholar
Stanisz, A. M., Befus, D. & Bienenstock, J. (1986). Differential effects of vasoactive intestinal peptide, substance P and somatostatin on immunoglobulin synthesis and proliferations by lymphocytes from Peyer's patches, mesenteric lymph nodes and spleen. Journal of Immunology 136, 152–156.Google Scholar
Stanisz, A. M. & Bienenstock, J. (1986). Neuropeptide modulation of mucosal immunity. Gastroenterology 91, 486.Google Scholar
Thomson, A. B. R., Keelan, M., Clandinin, M. T. & Walker, K. (1986). Dietary fat selectively alters transport properties of rat jejunum. Journal of Clinical Investigation 77, 279–288.Google Scholar