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Serum and intracellular retinol transport in the equine

Published online by Cambridge University Press:  24 July 2007

D. Sklan  and
Susan Donoghue
D. Sklan
Affiliation:
Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel
Susan Donoghue
Affiliation:
Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
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Abstract

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1. Serum and intracellular distribution of retinol was determined in equines maintained on four levels of vitamin A intake.

2. The form of retinol transported in serum was determined by gel filtration and chromatography to be a complex of retinol bound to a protein of molecular weight (MW) of approximately 20000, which was in turn complexed probably with prealbumin to yield a complex with a MW of 75000 to 80000.

3. Increasing dietary vitamin A levels enhanced the concentration of lipoprotein-bound retinyl esters in the plasma.

4. Vitamin A in the liver cytosol was found predominantly as retinyl esters in a lipid–protein aggregate of MW approximately 2 × 106 and hydrated density of 1·063–1·111. In the kidney and adrenal gland, two Iipid–protein entitites were found with MW of approximately 1·8 × 106 and 1·7 × 105 respectively. These fractions contained approximately 40 and 20% lipid respectively and had densities of 1·063–1·111 and approximately 1·21.

5. All lipid–protein aggregates were associated with retinyl palmitate hydrolase activity and guanidine treatment released a 15000 MW material, presumably intracellular retinol-binding protein.

6. Increasing dietary vitamin A enhanced the proportion of retinol in the 1·7 × 105 fraction.

7. Findings in equine plasma and liver resemble previous observations in other species. The characterization of two new lipid–protein aggregates in equine kidney and adrenal glands, which have hydrolase activity, may be important in intracellular retinol transport and metabolism, especially in animals subjected to high intakes of vitamin A.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 47 , Issue 2 , March 1982 , pp. 273 - 280
Copyright
Copyright © The Nutrition Society 1982

References

Augustin, J., Freeze, H., Tejada, P. & Brown, W. V. (1978). J. biol. Chem. 253, 2912.CrossRefGoogle Scholar
Belfrage, P. & Vaughan, M. (1969). J. Lipid Res. 10, 341.CrossRefGoogle Scholar
Chen, C. C. & Heller, J. (1979). Archs Biochem. Biophys. 198, 572.CrossRefGoogle Scholar
Donoghue, S., Kronfeld, D. S., Berkowitz, S. J. & Copp, R. L. (1981). J. Nutr. 111, 365.CrossRefGoogle Scholar
Glover, J., Jay, C. & White, G. H. (1974). Vitam. Horm. 32, 215.CrossRefGoogle Scholar
Harrison, E. H., Smith, J. E. & Goodman, D. S. (1979). J. Lipid Res. 20, 760.CrossRefGoogle Scholar
Heller, J. (1976). J. biol. Chem. 251, 2952.CrossRefGoogle Scholar
Heller, J. (1979). Archs Biochem. Biophys. 198, 562.CrossRefGoogle Scholar
McGuire, B. W. & Chytil, F. (1980). Biochem. biophys. Acta. 621, 324.Google Scholar
Mallia, A. K., Smith, J. E. & Goodman, D. S. (1975). J. Lipid Res. 16, 180.CrossRefGoogle Scholar
Muto, Y. & Goodman, D. S. (1972). J. biol. Chem. 247, 2553.Google Scholar
Muto, Y., Smith, F. R. & Goodman, D. S. (1973). J. Lipid Res. 14, 525.CrossRefGoogle Scholar
Ong, D. E. & Chytil, F. (1978). J. biol. Chem. 254, 8733.CrossRefGoogle Scholar
Petersen, P. A. (1971). J. biol. Chem. 246, 34.CrossRefGoogle Scholar
Ross, A. C., Takahashi, Y. I. & Goodman, D. S. (1978). J. biol. Chem. 253, 6591.CrossRefGoogle Scholar
Sklan, D. & Budowski, P. (1979). Lipids 14, 386.CrossRefGoogle Scholar
Sklan, D., Hurwitz, S., Budowski, P. & Ascarelli, I. (1975). J. Nutr. 105, 57.CrossRefGoogle Scholar
Smith, J. E. & Goodman, D. S. (1979). Fedn Proc. Fedn Am. Socs exp. Biol. 38, 60.Google Scholar