Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-24T05:07:14.707Z Has data issue: false hasContentIssue false
  • English
  • Français

The validation of using serum iron increase to measure iron absorption in human subjects

Published online by Cambridge University Press:  09 March 2007

Michael Hoppe ,
Lena Hulthén  and
Leif Hallberg
Michael Hoppe
Affiliation:
Department of Clinical Nutrition, Institute of Internal Medicine, Sahlgrenska Academy at Göteborg University, Sweden
Lena Hulthén*
Affiliation:
Department of Clinical Nutrition, Institute of Internal Medicine, Sahlgrenska Academy at Göteborg University, Sweden
Leif Hallberg
Affiliation:
Department of Clinical Nutrition, Institute of Internal Medicine, Sahlgrenska Academy at Göteborg University, Sweden
*
*Corresponding author: fax +46 31 82 94 75, Email Lena.Hulthen@medfak.gu.se
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The objectives of the present study were to study the correlation between the change in serum Fe and Fe absorption when administering 100 mg Fe (as FeSO4) orally, and to study the correlation between the absorption from a 3 mg and a 100 mg Fe (as FeSO4) dose. The study was conducted in a group of eleven male blood donors, without any evident infection, who had given blood 8 weeks before the study. On three consecutive mornings the subjects were served a wheat roll fortified with Fe. On the first 2 d the roll was fortified with 3 mg Fe labelled with 59Fe; on day 3 the roll was fortified with 100 mg Fe labelled with 55Fe. The serum Fe response to the 100 mg dose was followed for 6 h. Fe absorption was measured by whole-body counting. High correlations were seen between the absorption of Fe and the change in serum Fe after 100 mg Fe (r2 0·94, P>0·001), between the absorption from 3 mg and 100 mg Fe (r2 0·88, P>0·001), and between the absorption from 3 mg Fe and change in serum Fe after 100 mg Fe (r2 0·90, P>0·001). This strengthens the evidence that it is possible to use the change in serum Fe as a measure of Fe absorption, e.g. when establishing the relative bioavailability for Fe powders. The results also imply that the induced serum Fe increase following 100 mg Fe added to a food could predict the Fe absorption of a small dose of Fe added to the same meal.

Keywords

Serum ironIron absorptionRelative bioavailabilityElemental iron
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 485 - 488
Copyright
Copyright © The Nutrition Society 2004

References

Costa, A, Liberato, LN, Palestra, P & Barosi, GSmall-dose iron tolerance test and body iron content in normal subjects. Eur J Haematol (1991) 46, 152–157.CrossRefGoogle ScholarPubMed
Crosby, WH & O'Neil-Cutting, MAA small-dose iron tolerance test as an indicator of mild iron deficiency. J Am Med Assoc (1984) 251, 1986–1987.CrossRefGoogle ScholarPubMed
Ekenved, GAbsorption from different types of iron tablets – correlation between serum iron increase in total absorption of iron. Scand J Haematol (1976) 28, Suppl., 51–63.Google Scholar
Ekenved, G, Arvidsson, B & Solvell, LInfluence of food on the absorption from different types of iron tablets. Scand J Haematol (1976 a) 28, Suppl.79–88.Google Scholar
Ekenved, G, Norrby, A & Solvell, LSerum iron increase as a measure of iron absorption – studies on the correlation with total absorption. Scand J Haematol (1976 b) 28, Suppl.31–49.CrossRefGoogle ScholarPubMed
Gonzalez, H, Mendoza, C & Viteri, FEAbsorption of unlabeled reduced iron of small particle size from a commercial source. A method to predict absorption of unlabeled iron compounds in humans. Arch Latinoam Nutr (2001) 51, 217–224.Google ScholarPubMed
Hoppe, M, Hulthén, L & Hallberg, LSerum iron concentration as a tool to measure relative iron absorption from elemental iron powders. Scand J Clin Lab Invest (2003) 63, 489–496.CrossRefGoogle ScholarPubMed
Jensen, NM, Brandsborg, M, Boesen, AM, Yde, H & Dahlerup, JFLow-dose oral iron absorption test: establishment of a reference interval. Scand J Clin Lab Invest (1998) 58, 511–519.Google Scholar
Jensen, NM, Brandsborg, M, Boesen, AM, Yde, H & Dahlerup, JFLow-dose oral iron absorption test in anaemic patients with and without iron deficiency determined by bone marrow iron content. Eur J Haematol (1999) 63, 103–111.CrossRefGoogle ScholarPubMed
Joosten, E, Vander Elst, B & Billen, JSmall-dose oral iron absorption test in anaemic and non-anaemic elderly hospitalized patients. Eur J Haematol (1997) 58, 99–103.Google Scholar
Kelsey, SM, Hider, RC, Bloor, JR, Blake, DR, Gutteridge, CN & Newland, ACAbsorption of low and therapeutic doses of ferric maltol, a novel ferric iron compound, in iron deficient subjects using a single dose iron absorption test. J Clin Pharm Ther (1991) 16, 117–122.Google Scholar
Nielsen, JB, Ikkala, E, Solvell, L, Bjorn-Rasmussen, E & Ekenved, GAbsorption of iron from slow-release and rapidly-disintegrating tablets - a comparative study in normal subjects, blood donors and subjects with iron deficiency anaemiandash. Scand J Haematol (1976) 28, Suppl., 89–97.Google Scholar
Rossander, LEffect of dietary fiber on iron absorption in man. Scand J Gastroenterol (1987) 129, Suppl., 68–72.CrossRefGoogle ScholarPubMed
Wheby, MS & Umpierre, GEffect of transferrin saturation on iron absorption in man. N Engl J Med (1964) 271, 1391–1395.Google Scholar