Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-19T10:31:49.805Z Has data issue: false hasContentIssue false
  • English
  • Français

Application of an in vitro gas method to understand the effects of natural plant products on availability and partitioning of nutrients

Published online by Cambridge University Press:  27 February 2018

H. P. S. Makkar ,
M. Blümmel  and
K. Becker
H. P. S. Makkar
Affiliation:
Institute for Animal Production in the Tropics and Subtropics (480), University of Hohenheim, D-70593 Stuttgart, Germany
M. Blümmel
Affiliation:
Institute for Animal Production in the Tropics and Subtropics (480), University of Hohenheim, D-70593 Stuttgart, Germany
K. Becker
Affiliation:
Institute for Animal Production in the Tropics and Subtropics (480), University of Hohenheim, D-70593 Stuttgart, Germany
Get access

Extract

In vitromethods for laboratory estimation of food degradation are important tools for nutritionists.These methods either measure substrate disappearance by quantifying incubation residues or record fermentation products such as microbial biomass, short-chain fatty acids (SCFA) or gas volume (Blümmel et al., 1997a). Recently, the surge of interest in the efficient utilization of roughage diets has caused an increase in the use of gas methods because of the possibility of estimating the extent and rate of degradation in one sample by time series measurements of the accumulating gas volume. We combined gas measurements with residue determinations (truly degraded substrate) or microbial mass determination after fermentation to study the effects of natural plant products, in particular tannins and saponins, on the availability and partitioning of nutrients.

Type
Complex rumen transformations
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 147 - 150
Copyright
Copyright © British Society of Animal Science 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Balcells, J., Guada, J. A., Peiró, J. M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 575: 153–157.Google Scholar
Blümmel, M. and Becker, K. 1997. The degradability characteristics of fifty-four roughages and roughage neutral-detergent fibres as described by in vitro gas production and their relationship to voluntary feed intake. British Journal of Nutrition 77: 757–768.Google Scholar
Blümmel, M., Makkar, H. P. S. and Becker, K. 1997a. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition 77: 24–34.Google Scholar
Blümmel, M. and Ørskov, E. R. 1993. Comparison of in vitro gas production and nylon bag degradability of roughages in prediction of feed intake in cattle. Animal Feed Science and Technology 40: 109–119.Google Scholar
Blümmel, M., Steingass, H. and Becker, K. 1997b. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition 77: 911–921.Google Scholar
Makkar, H. P. S. and Becker, K. 1996. A bioassay for polyphenols (tannins). Polyphenol Communications 96, 15-18 July, 1996, Bordeaux, France, pp.197–198.Google Scholar
Makkar, H. P. S. and Becker, K. 1997. Nutritional implications of bound proanthocyanidins. Proceedings of the XVIII international grassland congress, June 8 - 12, Winnipeg, Canada.Google Scholar
Makkar, H. P. S., Blümmel, M. and Becker, K. 1995a. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques. British Journal of Nutrition 73: 897–913.Google Scholar
Makkar, H. P. S., Blümmel, M. and Becker, K. 1995b. In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. Journal of the Science of Food and Agriculture 69: 481–493.Google Scholar
Makkar, H. P. S., Blümmel, M. and Becker, K. 1997. In vitro rumen apparent and true digestibility of tannin-rich forages. Animal Feed Science and Technology In press.Google Scholar
Makkar, H. P. S. and Goodchild, A. V. 1996. Quantification of tannins: a laboratory manual. International Center for Agricultural Research in the Dry Areas (ICARDA).Google Scholar
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro . Journal of Agricultural Science, Cambridge 93: 217–222.Google Scholar
Raab, L., Cafantaris, B., Jilg, T. and Menke, K. H. 1983. Rumen protein degradation and biosynthesis. British Journal of Nutrition 50: 569–582.Google Scholar
Silanikove, N., Gilboa, N., Nir, I., Perevolotsky, A. and Nitsan, Z. 1996. Effect of a daily supplementation of polyethylene glycol on intake and digestion of tannincontaining leaves (Quercus calliprinos, Pistacia lentiscus, and Ceratonia siliqua)by goats. Journal of Agricultural and Food Chemistry 44: 199–205.Google Scholar