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The influence of antibiotics on gut bacteria diversity associated with laboratory-reared Bactrocera dorsalis

Published online by Cambridge University Press:  05 November 2018

Z. Bai ,
L. Liu ,
M.S. Noman ,
L. Zeng ,
M. Luo  and
Z. Li
Z. Bai
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
L. Liu
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
M.S. Noman
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
L. Zeng
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
M. Luo
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Z. Li*
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
*
*Author for correspondence Phone: 86-10-62733000 Fax: 86-10-62733404 E-mail: lizh@cau.edu.cn
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Abstract

The oriental fruit fly Bactrocera dorsalis (Hendel) is a destructive insect pest of a wide range of fruit crops. Commensal bacteria play a very important part in the development, reproduction, and fitness of their host fruit fly. Uncovering the function of gut bacteria has become a worldwide quest. Using antibiotics to remove gut bacteria is a common method to investigate gut bacteria function. In the present study, three types of antibiotics (tetracycline, ampicillin, and streptomycin), each with four different concentrations, were used to test their effect on the gut bacteria diversity of laboratory-reared B. dorsalis. Combined antibiotics can change bacteria diversity, including cultivable and uncultivable bacteria, for both male and female adult flies. Secondary bacteria became the dominant population in female and male adult flies with the decrease in normally predominant bacteria. However, in larvae, only the predominant bacteria decreased, the bacteria diversity did not change a lot, likely because of the short acting time of the antibiotics. The bacteria diversity did not differ among fruit fly treatments with antibiotics of different concentrations. This study showed the dynamic changes of gut bacterial diversity in antibiotics-treated flies, and provides a foundation for research on the function of gut bacteria of the oriental fruit fly.

Keywords

Bactrocera dorsalisantibioticbacteria diversitygutlaboratory
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 4 , August 2019 , pp. 500 - 509
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Copyright
Copyright © Cambridge University Press 2018 

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Footnotes

†

These authors contributed equally to this work.

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