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Proteomic research on diapause-related proteins in the female ladybird, Coccinella septempunctata L.

Published online by Cambridge University Press:  25 November 2015

X.Y. Ren ,
L.S. Zhang ,
Y.H. Han ,
T. An ,
Y. Liu ,
Y.Y. Li  and
H.Y. Chen
X.Y. Ren
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
L.S. Zhang*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y.H. Han
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
T. An
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y. Liu
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y.Y. Li
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
H.Y. Chen
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
*
*Author for correspondence Phone: +86-010-8210-9581 Fax: +86-10-82105926 E-mail: zhangleesheng@163.com
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Abstract

In the experiments reported here, we used the female ladybird Coccinella septempunctata L. as a model to identify diapause-associated proteins using proteomics technology. Our results indicated that protein expression patterns of diapausing and nondiapausing individuals were highly differentiated. A total of 58 spots showed significant differences in abundance (Ratio > 2 and P < 0.05) according to two-dimensional electrophoresis and GE Image Scanner III analysis. Sixteen protein spots were further investigated using mass spectrometry. Eight proteins were characterized, including chaperones and proteins involved in glucose metabolism, lipid metabolism, and the tricarboxylic acid cycle. Among these proteins, five proteins were upregulated in diapausing female adults, including a chaperone (Symbionin symL), malate dehydrogenase (putative), two proteins linked to lipid metabolism (unknown and conserved hypothetical protein) and phosphoglyceromutase (partial). By contrast, isocitrate dehydrogenase (RH49423p), fumarylacetoacetate hydrolase (AGAP001942-PA), and a putative medium chain acyl-CoA dehydrogenase were downregulated. These results contribute to the understanding of diapause mechanisms of the ladybird C. septempunctata and may suggest methods for improving the application of this natural enemy insect.

Keywords

diapause associated proteinsCoccinella septempunctatafemale adultstwo-dimensional electrophoresis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 2 , April 2016 , pp. 168 - 174
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Copyright
Copyright © Cambridge University Press 2015 

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