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A rapid radioimmunoassay for the detection of Mansonia antigen (Diptera: Culicidae): its potential use as a sensitive method for studying predator- prey relationships1

Published online by Cambridge University Press:  10 July 2009

Donald L. Bailey ,
Angela L. Choate  and
Michael J. P. Lawman
Donald L. Bailey
Affiliation:
Insects Affecting Man and Animals Research Laboratory, USDA, Agricultural Research Service, Gainesville, Florida 32604, USA
Angela L. Choate
Affiliation:
Insects Affecting Man and Animals Research Laboratory, USDA, Agricultural Research Service, Gainesville, Florida 32604, USA
Michael J. P. Lawman
Affiliation:
Insects Affecting Man and Animals Research Laboratory, USDA, Agricultural Research Service, Gainesville, Florida 32604, USA
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Abstract

A radioimmunoassay (RIA) was developed that utilizes 125I-linked Protein A as a tracer to detect the presence of antibodies to an antigen of Mansonia larvae. Antiserum was made by periodic injections of ground gut of fourth-instar larvae of M. dyari Belkin, Heinemann & Page into a rabbit, bleeding the rabbit by cardiac puncture and separating the serum. Using the rabbit antiserum at various dilutions, the specificity of the antibody to Mansonia was examined in tests with seven other mosquito species of different genera. Although there was low-level cross-reactivity with all the other species, the differences in reactivity between those and Mansonia larvae ranged between 2·5 and 8·4-fold; therefore, positive identification was possible. Furthermore, one fourth-instar Mansonia larva could be detected within a predator (fourth-instar larvae of Toxorhynchites rutilus rutilus (Coquillett) 48 h after consumption using the RIA.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 76 , Issue 1 , March 1986 , pp. 141 - 150
Copyright
Copyright © Cambridge University Press 1986

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