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A combination of molecular and morphological approaches resolves species in the taxonomically difficult genus Procladius Skuse (Diptera: Chironomidae) despite high intra-specific morphological variation

Published online by Cambridge University Press:  10 March 2011

M.E. Carew ,
S.E. Marshall  and
A.A. Hoffmann
M.E. Carew*
Affiliation:
Victorian Centre for Aquatic Pollution Identification and Management (CAPIM), Department of Zoology, Bio21 Institute, The University of Melbourne, 3010, Australia
S.E. Marshall
Affiliation:
Victorian Centre for Aquatic Pollution Identification and Management (CAPIM), Department of Zoology, Bio21 Institute, The University of Melbourne, 3010, Australia
A.A. Hoffmann
Affiliation:
Victorian Centre for Aquatic Pollution Identification and Management (CAPIM), Department of Zoology, Bio21 Institute, The University of Melbourne, 3010, Australia
*
*Author for correspondence Fax: 61 03 8344 2279 E-mail: mecarew@unimelb.edu.au
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Abstract

Molecular approaches for identifying aquatic macroinvertebrate species are increasingly being used but there is ongoing debate about the number of DNA markers needed to differentiate species accurately. Here, we use two mitochondrial genes (cytochrome oxidase I, cytochrome b) and a nuclear gene (carbamoylphosphate synthetase) to differentiate species variation within the taxonomically challenging chironomid genus Procladius from southern Australia, a genus which is important for pollution monitoring. The mitochondrial genes indicated cryptic species that were subsequently linked to morphological variation at the larval and pupal stage. Two species previously described based on morphological criteria were linked to molecular markers, and there was evidence for additional cryptic species. Each genetic marker provided different information, highlighting the importance of considering multiple genes when dissecting taxonomically difficult groups, particularly those used in pollution monitoring.

Keywords

DNA identificationPCR-RFLPbarcodingspecies discriminationchironomid
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 5 , October 2011 , pp. 505 - 519
Copyright
Copyright © Cambridge University Press 2011

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