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ELECTROPHORETIC DETECTION OF PARASITISM BY DRYINIDAE IN TYPHLOCYBINAE LEAFHOPPERS (HOMOPTERA: AUCHENORRHYNCHA)

Published online by Cambridge University Press:  31 May 2012

Stefano Demichelis  and
Aulo Manino
Stefano Demichelis
Affiliation:
DI.VA.P.R.A.-Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano”, Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco (Torino), Italy
Aulo Manino
Affiliation:
DI.VA.P.R.A.-Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano”, Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco (Torino), Italy
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Abstract

Nine species of the subfamily Typhlocybinae (Auchenorrhyncha: Cicadellidae) were collected from Corylus avellana L., Solatium tuberosum L., and Vitis vinifera L. Seven species were parasitized by Dryinidae, among which Alebra coryli Le Quesne 1977 and Edwardsiana staminata (Ribaut, 1931) were recognized for the first time. Clearly parasitized and apparently unparasitized leafhoppers and dissected dryinid larvae were assayed by polyacrylamide gel electrophoresis. The α-glycerophosphate dehydrogenase (α-GPD) enzyme system proved to be a reliable tool for indicating the presence of dryinids in all sampled species: the parasitized adults always showed a fast band, which was absent in unparasitized adults. The α-GPD was a reliable enzyme system to detect dryinids at an early stage of their development but not to discriminate among dryinid species. The occurrence of dryinids in the males of A. coryli and Zygina rhamni Ferrari, 1882 was also pointed out by the change of their colouration.

Résumé

Neuf espèces de la sousfamille Typhlocybinae (Auchenorrhyncha : Cicadellidae) ont été capturées sur Corylus avellana L., Solarium tuberosum L. et Vitis vinifera L. Le parasitisme par Dryinidae a été observé dans sept espèces, et pour la première fois même dans Alebra coryli Le Quesne 1977 et Edwardsiana staminata (Ribaut, 1931). Les adultes de Typhlocybinae avec le parasite, sans parasite et les larves des dryinidés détachées par l’hôte, ont été utilisés dans une analyse des isoenzymes en gel vertical de polyacrylamide. La présence des dryinidés dans les adultes a été démontrée par l’enzyme α-GPD. Tous les adultes avec le parasite avaient une bande très vite, qui manquait dans les adultes sans parasite. Partant avec l’enzyme α-GPD il est possible de repérer les dryinidés lorsque leur sac larvaire. n’est pas bien développé. Le même enzyme n’est pas d’utilité pour l’identification des différentes espèces des dryinidés. La présence de ces parasites a fait apparaître la pigmentation des femelles dans les maies de A. coryli et Zygina rhamni Ferrari, 1882.

Type
Articles
Information
The Canadian Entomologist , Volume 130 , Issue 4 , August 1998 , pp. 407 - 414
Copyright
Copyright © Entomological Society of Canada 1998

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References

Arzone, A., Vidano, C., and Arno, C.. 1988. Predators and parasitoids of Empoasca vitis and Zygina rhamni (Rhynchota Auchenorrhyncha). pp. 623–629in Proceedings of the 6th Auchenorrhyncha Meeting, Turin, Italy, 7–11 September 1987.Google Scholar
Camussi, A., Möller, F., Ottaviano, E., and Gorla, M. Sari. 1986. Metodi statistici per la sperimentazione biologica. Zanichelli, Bologna.Google Scholar
Claridge, M.F., and Wilson, M.R.. 1981. Host plant association, diversity and species-area relationships of mesophyll-feeding leafhoppers of trees and shrubs in Britain. Ecological Entomology 6: 217–238.CrossRefGoogle Scholar
Demichelis, S., and Bosco, D.. 1995. Host-plant relationships and life history of some Alebra species in Italy (Auchenorrhyncha: Cicadellidae). European Journal of Entomology 92: 683–690.Google Scholar
Demichelis, S., and Manino, A.. 1995. Electrophoretic detection of dryinid parasitoids in Empoasca leafhopper. Journal of Applied Entomology 119: 543–545.CrossRefGoogle Scholar
Gillham, M. 1991. Polymorphism, taxonomy and host plant associations in Alebra leafhoppers (Homoptera: Cicadellidae: Typhlocybinae). Journal of Natural History 25: 233–255.CrossRefGoogle Scholar
Gillham, M. 1992. Variation in acoustic signals within and among leafhopper species of the genus Alebra (Homoptera, Cicadellidae). Biological Journal of the Linnean Society 45: 1–15.CrossRefGoogle Scholar
Jervis, M.A. 1980. Ecological studies on the parasite complex associated with typhlocybinae leafhoppers (Homoptera, Cicadellidae). Ecological Entomology 5: 123–136.CrossRefGoogle Scholar
Le Quesne, W.J. 1977. A new species of Alebra Fieber (Hem. Cicadellidae). Entomologist's Monthly Magazine 112: 49–52.Google Scholar
Le Quesne, W.J., and Payne, K.. 1981. Cicadellidae (Typhlocybinae) with a check list of the British Auchenorrhyncha (Hemiptera, Homoptera). pp. 8–10in Fitton, M.G. (Ed.), Handbook for the Identification of British Insects Vol. 2 (2). Royal Entomological Society, London.Google Scholar
Nast, J. 1972. Palaeartic Auchenorrhyncha (Homoptera). An annotated check list. Polish Scientific Publishers, Warszawa.Google Scholar
Olmi, M. 1984. A revision of the Dryinidae (Hymenoptera). Memoirs of the American Entomological Institute 37 (1): 1–946; 38 (2): 947–1913.Google Scholar
Olmi, M. 1989. Ricerche sui Dryinidae limitanti lo sviluppo di Auchenorrhinchi in ecosistemi mediterranei: ospiti, parassitoidi e percentuali di parassitizzazione (Hymenoptera, Chrysidoidea). Frustula Entomologica (N.S.) 9 (22): 223–232.Google Scholar
Powell, W., Walton, M.P., and Jervis, M.A.. 1996. Population and communities. pp. 223–292in Jervis, M.A., and Kidd, N. (Eds.), Insect Natural Enemies. Chapman and Hall Ltd., London.CrossRefGoogle Scholar
Ribaut, H. 1936. Homopteres Auchénorhynques. I. (Typhlocybidae). Faune de France 31.Google Scholar
Tavella, L., Alma, A., and Arzone, A.. 1994. Ricerche bio-ecologiche su Aphelopus atratus (Dalman) e A. serratus Richards, (Dryinidae Aphelopinae) parassitoidi di Zygina rhamni Ferrari. Memorie della Società Entomologiça Italiana Genova 72: 189–194.Google Scholar
Vidano, C., and Arzone, A.. 1987. Typhlocybinae of broadleaved trees and shrubs in Italy. 4. Fagaceae. Bollettino dell'Istituto di Entomologia della Università degli Studi di Bologna 70: 171–189.Google Scholar
Waloff, N., and Jervis, M.A.. 1987. Communities of parasitoids associated with leafhoppers and planthoppers in Europe. Advances in Ecological Research 17: 281–402.CrossRefGoogle Scholar