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Molecular cloning, characterization, and expression analysis of a QM homologue in the ant Polyrhachis vicina (Hymenoptera: Formicidae)

Published online by Cambridge University Press:  02 April 2012

Shumin Lü ,
Gengsi Xi  and
Xiaohui Wang
Shumin Lü
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
Gengsi Xi*
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
Xiaohui Wang
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
*
1Corresponding author (e-mail: Xigengsi@snnu.edu.cn).
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Abstract

QM, a tumor-suppressor gene, plays an important role in cell growth, differentiation, and apoptosis. In this report, a homologue of human QM was isolated from the ant Polyrhachis vicina Roger. The full-length cDNA of P. vicina QM (PvQM) is 827 base pairs (bp) and contains a 5′-untranslated region of 91 bp and a 3′-untranslated region of 77 bp. The open reading frame of PvQM encodes a deduced 219-amino acid peptide with a predicted molecular mass of 25.1 kilodaltons. The results of sequence alignments indicate that the PvQM protein shares an overall identity of 76.7%–98.2% with other known QM homologues, and is most closely related to that of Apis mellifera L. (Hymenoptera: Apidae). Real-time quantitative reverse transcription - polymerase chain reaction was performed to compare PvQM mRNA expression during P. vicina development and within different castes. The data revealed that PvQM mRNA is differentially expressed during P. vicina development, with the highest expression level in embryos and the lowest in late-instar larvae and pupae. The levels of PvQM transcripts also vary among castes, with higher levels in workers and lower levels in both males and females. These results suggest that PvQM is developmentally and caste-specifically regulated at the level of transcription.

Résumé

Le gène suppresseur de tumeurs QM joue un rôle important dans la croissance cellulaire, la différenciation et l’apoptose. Dans notre travail, nous avons isolé un homologue du QM humain chez la fourmi Polyrhachis vicina Roger. La longueur totale de l’ADN complémentaire du QM de P. vicina (PvQM) est de 827 paires de bases (bp) avec une région non transcrite en 5′ de 91 bp et une région non transcrite en 3′ de 77 bp. Le cadre ouvert de lecture (ORF) de PvQM code un peptide déduit de 219 acides aminés de masse moléculaire prédite de 25,1 kilodaltons. L’alignement des séquences indique que la protéine PvQM possède une identité globale commune de 76,7 % – 98,2 % avec les autres homologues connus de QM et qu’elle est le plus apparentée à celle d’Apismellifera L. (Hymenoptera: Apidae). Une analyse quantitative en temps réel d’amplification en chaîne par polymérase a permis de comparer l’expression de l’ARNm de PvQM durant le développement de P. vicina et au sein des diverses castes. Nos données montrent que l’ARNm de PvQM est exprimé de façon différente au cours du développement de P. vicina, l’expression maximale se retrouvant chez les embryons et la plus faible chez les larves de derniers stades et les nymphes. Les valeurs des produits de transcription de PvQM varient aussi en fonction des castes et sont plus élevées chez les ouvrières et moins élevées chez les fourmis, tant mâles que femelles. Nos résultats indiquent une régulation spécifique de PvQM au niveau de la transcription en fonction du stade de développement et de la caste.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 140 , Issue 3 , June 2008 , pp. 312 - 323
Copyright
Copyright © Entomological Society of Canada 2008

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